The Role of Intermolecular Forces on Fractal Aggregation and Fusion Process of Vesicles

分子间力对囊泡分形聚集和融合过程的作用

• 批准号: 05403012
• 负责人: IMAE Toyoko
• 金额: $ 23.3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05403012/
• 关键词: vesicle; fusion; fractal aggregation; electron microscope; atomic force microscope; Langmuir-Blodgett film; surface force apparatus; small-angle neutron scattering; 分子間力直接測定装置; 分子間力; 走査プローブ顕微鏡

The intermolecular interactions which contribute to the fractal aggregation and the fusion process of vesicles in water were investigated for the purpose of making sure of the reaction mechanism of cell segmentation and fusion in an organism. When fatty acid analogs were added in lipid vesicles, the gel-liquid crystal transition were examined. The structure of vesicles constructed by lipid, fatty acid, and the relating amphiphilic molecules was observed by optical and electron microscopes and determined by light scattering and small-angle neutron scattering. These investigation indicates that the character of vesicle membrane depends on the character of additives. The fractal aggregation of crystal islands on Langmuir-Blodgett (LB) membrane of C_<60> fullerene molecules was observed by the atomic force microscope. This is the model investigation of the effect of interparticle interaction on the aggregation process of small particles. The functionality of vesicles is strongly related to the stability of bilayrs. Therefore, amphiphiles with bilayr formation ability were two-dimentionally organized by LB method. The experimental result suggests that the interaction induced by the hydrophilic atmosphere affects to the fractal aggregation and the fusion process of vesicles.

为明确生物体细胞分裂与融合的反应机理，研究了水溶液中囊泡分形聚集与融合过程中的分子间相互作用。当脂肪酸类似物添加到脂质囊泡中时，检查凝胶-液晶转变。通过光学显微镜和电子显微镜观察了脂质、脂肪酸和相关两亲性分子构建的囊泡的结构，并通过光散射和小角中子散射确定了囊泡的结构。这些研究表明，囊泡膜的性质取决于添加剂的性质。用<60>原子力显微镜观察了C_富勒烯分子在LB膜上晶岛的分形聚集。这是研究颗粒间相互作用对小颗粒聚集过程影响的模型研究。囊泡的功能与双层膜的稳定性密切相关。因此，具有双层形成能力的两亲分子可以通过LB方法进行二维组装。实验结果表明，亲水气氛诱导的相互作用影响了囊泡的分形聚集和融合过程。

项目成果

Toyoko Imae: Light Scattering method. in "Colloid Science Experimental Methods" Ed. by Japan Chemical Society. Tokyo Kagaku Dojin, (1996)

今前东横：光散射法。

松村秀敏 他3名: "蛍光偏光度法によるジカルボン酸型界面活性剤/ジミリストイル-3-sn-ホスファチジルコリン混合ベシクルの膜流動性の評価" 油化学. 43. 243-250 (1994)

松村秀俊等3人：“通过荧光偏振法评价二羧酸型表面活性剂/二肉豆蔻酰-3-sn-磷脂酰胆碱混合囊泡的膜流动性”《石油化学》43. 243-250 (1994)。

今栄東洋子 他4名: "Self-assembly Formation of Amphiphilic Molecules Mixed with Photoreactive,aromatic unfaturated-acids:Examination by Light scattering" Colloid Polym.Sci.(印刷中). (1995)

Toyoko Imaei 和其他 4 人：“与光反应性芳香族不饱和酸混合的两亲性分子的自组装形成：光散射检查”胶体聚合物科学（1995 年出版）。

加藤貞二 他15名: "コロイド科学実験法" 東京化学同人, (1996)

加藤 Teiji Kato 等 15 人：《胶体科学实验方法》东京化学同人，（1996）

高木克彦 他4名: "Organized Photodimerization of Unsaturated Carbcylates.Selectivity Contool by Normal and Roversed Miulles" J.Chem.Soc.Perkin Trans.2. 1003-1009 (1994)

Katsuhiko Takagi 和其他 4 人：“不饱和碳酸盐的有机光二聚化。Normal 和 Roversed Miulles 的选择性控制”J.Chem.Soc.Perkin Trans.2 (1994)。