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Non-invasive measurements of sensory nerve and cortical responses to skin stimulation : Integration of EEG,MEG and fMRI

无创测量感觉神经和皮质对皮肤刺激的反应：脑电图、脑磁图和功能磁共振成像的整合

基本信息

批准号：
05404087
负责人：
HASHIMOTO Isao
金额：
$ 19.01万
依托单位：
Tokyo Institute of Psychiatry
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1996
项目状态：
已结题

项目摘要

1. We developed 3 point air-puff stimulators which can be controlled independently for eliciting sensory nerve action potentials and somatosensory evoked potentials.2. We developed 3 point vibratory stimulators for studying somatosensory evoked fields associated with perception of frequency, duration and intensity in the somatosensory system.3. We visualized displacements of the skin following air-puff stimulation with a high speed camera which were correlated with a sensory threshold and magnitude estimations.4. We visualized a moving quadrupole along the median nerve with multi-chnnel measurements of action fields over the elbow.5. In peripheral nerve lesions, we found a peculiar oscillation pattern of electrically evoked action fields at or distal to the lesion sites. We performed a computer simulation for the oscillation using the Hodgikin-Huxley equations and found that the increase of Na conductance was related to the oscillation.6. We measured magnetic fields following trigemina … More l stimulation from the decorticated piglets and showed that the subcortically generated fields are strong enough to the detectable outside the head.7. We measured N20m primary response during wakefulness and sleep with subsequent digital high-pass (>300 Hz) and low-pass (>300 Hz) filtering. The estimated source for the high-frequency oscillations (HFOs) was the area 3b very close to that for the N20m. During sleep, the HFOs showed a dramatic diminution in amplitude while the N20m amplitude exhibited a moderate increment. This reciprocal relation between the HFOs and the N20m during a wake-sleep cycle suggests that they represent opposing generator substrates. We speculate that the HFOs represent a localized activity of the GABAergic inhibitory interneurons of layr 4, which have been shown in animal experiments to respond monosynaptically to thalamo-cortical input with a high-frequency (600-900 Hz) burst of short duration spikes. On the other hand, the underlying N20m represents activity of pyramidal neurons which receive monosynaptic excitatory input from the thalamus as well as a feed-forward inhibition from the interneurons. This hypothesis has been further supported by our recent experiments manipulating the amplitude of N20m during wakefulness ; the reciprocal relation between HFOs and N20m hold not only during a wake-sleep cycle but also during wakefulness. Less

1．开发了可独立控制的三点喷气刺激器，用于诱发感觉神经动作电位和体感诱发电位。2．我们开发了三点振动刺激器，用于研究与体感系统中频率、持续时间和强度感知相关的体感诱发场。3．我们用高速摄像机观察了吹气刺激后皮肤的位移，该位移与感觉阈值和幅度估计相关。4。我们通过对肘部作用场的多通道测量，可视化了沿正中神经移动的四极杆。5。在周围神经病变中，我们发现病变部位或其远端的电诱发作用场具有特殊的振荡模式。我们利用Hodgikin-Huxley方程对振荡进行了计算机模拟，发现Na电导的增加与振荡有关。 6．我们测量了去皮仔猪三叉神经刺激后的磁场，结果表明皮质下产生的磁场足以在头部外部检测到。7。我们通过随后的数字高通（>300 Hz）和低通（>300 Hz）滤波测量了清醒和睡眠期间的 N20m 主要响应。高频振荡 (HFO) 的估计来源是区域 3b，非常接近 N20m。在睡眠期间，HFO 的振幅急剧减小，而 N20m 振幅则适度增加。在唤醒-睡眠周期期间，HFO 和 N20m 之间的这种相互关系表明它们代表相反的发生器基质。我们推测，HFO 代表了第 4 层 GABA 能抑制性中间神经元的局部活动，动物实验表明，这些中间神经元可以通过短时间尖峰的高频（600-900 Hz）爆发对丘脑皮质输入做出单突触反应。另一方面，底层的 N20m 代表锥体神经元的活动，锥体神经元接收来自丘脑的单突触兴奋性输入以及来自中间神经元的前馈抑制。我们最近在清醒期间操纵 N20m 幅度的实验进一步支持了这一假设； HFO 和 N20m 之间的相互关系不仅在清醒-睡眠周期中成立，而且在清醒期间也成立。较少的