RNA-binding proteins rewire transcriptomes in immune cell differentiation

RNA结合蛋白在免疫细胞分化中重新连接转录组

• 批准号: DP220101388
• 负责人: A/Prof Jiayu Wen
• 金额: $ 34.13万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2022
• 资助国家: 澳大利亚
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP220101388
• 关键词: RNA; binding; proteins; rewire; transcriptomes

This project aims to combine advanced computational and experimental techniques to investigate a new layer of gene regulation by novel RNA binding proteins (RBP) which control messenger RNA length in immune cells. This project expects to demonstrate that these RBPs have a profound effect on immune cell differentiation and response to infection. Expected outcomes include the discovery of new RBPs regulating immunity, with mechanism and function determined by novel CRISPR editing of a transgenic mouse model. The significant benefit will be a more complete understanding of RNA mechanisms of immune response, which will be critical in informing future advances in the rapidly developing areas of RNA-based biotechnologies and synthetic immunology.

该项目旨在结合先进的计算和实验技术，通过控制免疫细胞中信使RNA长度的新型RNA结合蛋白(RBP)来研究新的基因调控层。该项目希望证明这些限制性商业惯例对免疫细胞分化和对感染的反应具有深远的影响。预期的结果包括发现新的限制性商业惯例调节免疫，其机制和功能由转基因小鼠模型的新CRISPR编辑确定。重要的好处将是对免疫反应的RNA机制有更全面的了解，这将对基于RNA的生物技术和合成免疫学快速发展领域的未来进展起到至关重要的作用。