Evolution and functional impact of gene silencing by hairpin derived RNAs

发夹来源的 RNA 基因沉默的进化和功能影响

• 批准号: FT160100143
• 负责人: A/Prof Jiayu Wen
• 金额: $ 47.65万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-08-15 至 2021-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT160100143
• 关键词: Evolution; functional; impact; gene; silencing

This project aims to study RNA-mediated gene silencing in genome evolution. RNA interference (RNAi) has been widely used as an experimental tool since its Nobel Prize-winning discovery in 1998, but little is known about endogenous RNAi or its evolution. This project uses bioinformatics, high-throughput sequencing and molecular approaches to study hpRNAs, a class of small interfering RNAs, their adaptive evolution across fly species and vertebrates, and their functional effect on testis morphogenesis and distortion of female/male sex-ratio. The project also studies splicing-dependent small RNAs and miRNA-target interaction. This research could have applications from animal development to human pathology.

本项目旨在研究基因组进化中RNA介导的基因沉默。自1998年获得诺贝尔奖以来，RNA干扰（RNAi）被广泛用作实验工具，但对内源性RNAi及其进化知之甚少。本项目利用生物信息学、高通量测序和分子生物学方法研究hpRNA（一类小干扰RNA）在蝇类和脊椎动物中的适应性进化及其对睾丸形态发生和雌雄性别比畸变的功能影响。该项目还研究了剪接依赖性小RNA和miRNA-靶标相互作用。这项研究可以从动物发展应用到人类病理学。