Visualized measurement of cell by laser and fluorescence dye, -Its application to immunology and cancer treatment-

通过激光和荧光染料对细胞进行可视化测量，-其在免疫学和癌症治疗中的应用-

• 批准号: 06044027
• 负责人: FUJII Kanichi
• 金额: $ 9.79万
• 依托单位: Ibaraki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044027/
• 关键词: ILISPOT; immunoassay; cytokine; IDIP; automation; pattern recognition; laser; Fluorescent dye; バイオメディカル; ILISPOT法; 免疫学; 粘膜免疫機構; 抗原特異抗体; 免疫担当細胞; T細胞

This project among Ibaraki University, University of Alabama at Birmingham, and Stanford University was started at 1994. The purpose of this project is thedevelopment of new automatic immunoassay system which removed human errors. Although the purpose was not accomplished perfectly, the semi-automatic system was performed.The concrete results were summarized as follows :1.The automation of hard-ware of ILISPOT-IDIP system by computer controllThe automatic adressing of the stage of the microscope using the combination of stepping motor and computer was accomplished. Although the image processing system is semi-sutomatic at this time, the automatic recognition between real spots and psuede spots was performed.Concequently, it is thought that the performance of the ILISPOT-IDIP system attains relatively high level as the automatic immunoassy system.2.The application of color center laser as the light source of ILISPOT system was thought effective for the enhancement of the ditecting limitaion of the spots. Hpwever, the instrument of color center laser is so huge. The investigation of the down-sizing of the instrument now in progress.3.Three kinds of new fluoorine-containing fluorescent dyes (modified BODIPY) was prepared. Although the dyes have lipophilicity, solubility in water and high intensity of emmition, the study of the combination of the dyes with proteins or DNA is now in progress.

该项目由茨木大学、伯明翰的亚拉巴马大学和斯坦福大学于1994年启动。本课题的目的是研制一种消除人为误差的新型自动免疫分析系统。1.利用计算机实现ILISPOT-IDIP系统硬件的自动化利用步进电机与计算机的结合，实现了显微镜载物台的自动定位。虽然图像处理系统目前还处于半成熟状态，但已实现了真实的斑点与伪斑点的自动识别，因此认为ILISPOT-IDIP系统的性能达到了自动免疫分析系统的较高水平。2.采用色心激光作为ILISPOT系统的光源，有效地提高了斑点的检测限。然而，色心激光的仪器是如此巨大。3.合成了三种新型含氟荧光染料（改性BODIPY）。虽然这些染料具有亲油性、水溶性和高发光强度，但它们与蛋白质或DNA结合的研究正在进行中。

项目成果

藤井 寛一: "Comparison of A.C.Driven Barrier Discharge with D.C.Driven Malter Effect Corona Discharges for Plasma Reactor" Proceeding of 11th International Conference on Gas Dis charges and Their Applications. 1. 164-167 (1995)

Kanichi Fujii：“等离子体反应器的交流驱动势垒放电与直流驱动马尔特效应电晕放电的比较”第 11 届气体放电及其应用国际会议论文集 1. 164-167 (1995)。

藤井 寛一: "A Proposal of Modifying the Quittner Method for Gamma-Ray Peak Area Determination" Radio Isotopes. 45. 92-94 (1996)

Kanichi Fujii：“修改用于伽马射线峰面积测定的 Quitner 方法的提案”放射性同位素 45. 92-94 (1996)。

Okahashi,N.,Yamamoto,M.,Vancott,J.L.,Charfield,S.N.,Roberts,M.,Bluethmann,H.,Hiroi,T.,Kiyono,H.and McGhee,J.R.: "Mucosal immunity in IL-4 knockout mice : oral administration of recombinant Salmonella or cholea toxin elicits CD4^+ Th2 cells producing IL-6

Okahashi,N.、Yamamoto,M.、Vancott,J.L.、Charfield,S.N.、Roberts,M.、Bluethmann,H.、Hiroi,T.、Kiyono,H. 和 McGhee,J.R.：“IL-4 敲除中的粘膜免疫

Fujihashi,K.,McGhee,J.R.,Kweon,M-N,Cooper,M.D.,Tonegawa,S.,Takahashi,I.,Hiroi,T.,Mestecky,J.and Kiyono,H.: "γδ cell deficient mice have imparied mucosal IgA responses" J.Exp.Med.183. 1929-1935 (1996)

Fujihashi, K.、McGhee, J.R.、Kweon, M-N、Cooper, M.D.、Tonekawa, S.、Takahashi, I.、Hiroi, T.、Mestecky, J. 和 Kiyono, H.：“γδ 细胞缺陷小鼠的粘膜功能受损IgA 反应”J.Exp.Med.183. 1929-1935 (1996)

S.Kamemaru: "Universal patern recognition by matched filters synthesized by primitive patterns and by the algorithm for uniquely selecting the optimum reference patterns" Proc.of SPIE. 2565. 232-239 (1995)

S.Kamemaru：“通过由原始模式和唯一选择最佳参考模式的算法合成的匹配过滤器进行通用模式识别”Proc.of SPIE。