Visualized measurement of cell by laser and fluorescence dye, -Its application to immunology and cancer treatment-

这个由茨城大学、伯明翰阿拉巴马大学和斯坦福大学合作的项目始于1994年。该项目的目的是开发新的自动免疫分析系统，消除人为错误。虽然目的没有完全实现，但半自动系统还是实现了。具体结果总结如下：1。采用计算机控制实现了ILISPOT-IDIP系统硬件的自动化，采用步进电机与计算机相结合的方式实现了显微镜台的自动寻址。虽然此时的图像处理系统是半自动的，但实现了对真实斑点和伪斑点的自动识别。因此，人们认为ILISPOT-IDIP系统作为一种自动免疫分析系统的性能达到了较高的水平。采用色心激光器作为ILISPOT系统的光源，可以有效地提高光斑的定向限制。然而，色心激光器的仪器是如此巨大。缩小仪器尺寸的调查正在进行中。制备了三种新型含氟荧光染料（改性BODIPY）。虽然染料具有亲脂性、水溶性和高发射强度，但与蛋白质或DNA结合的研究目前仍在进行中。

This project among Ibaraki University, University of Alabama at Birmingham, and Stanford University was started at 1994. The purpose of this project is thedevelopment of new automatic immunoassay system which removed human errors. Although the purpose was not accomplished perfectly, the semi-automatic system was performed.The concrete results were summarized as follows :1.The automation of hard-ware of ILISPOT-IDIP system by computer controllThe automatic adressing of the stage of the microscope using the combination of stepping motor and computer was accomplished. Although the image processing system is semi-sutomatic at this time, the automatic recognition between real spots and psuede spots was performed.Concequently, it is thought that the performance of the ILISPOT-IDIP system attains relatively high level as the automatic immunoassy system.2.The application of color center laser as the light source of ILISPOT system was thought effective for the enhancement of the ditecting limitaion of the spots. Hpwever, the instrument of color center laser is so huge. The investigation of the down-sizing of the instrument now in progress.3.Three kinds of new fluoorine-containing fluorescent dyes (modified BODIPY) was prepared. Although the dyes have lipophilicity, solubility in water and high intensity of emmition, the study of the combination of the dyes with proteins or DNA is now in progress.