Visualized measurement of cell by laser and fluorescence dye, -Its application to immunology and cancer treatment-

该项目由茨木大学、伯明翰的亚拉巴马大学和斯坦福大学于1994年启动。本课题的目的是研制一种消除人为误差的新型自动免疫分析系统。1.利用计算机实现ILISPOT-IDIP系统硬件的自动化利用步进电机与计算机的结合，实现了显微镜载物台的自动定位。虽然图像处理系统目前还处于半成熟状态，但已实现了真实的斑点与伪斑点的自动识别，因此认为ILISPOT-IDIP系统的性能达到了自动免疫分析系统的较高水平。2.采用色心激光作为ILISPOT系统的光源，有效地提高了斑点的检测限。然而，色心激光的仪器是如此巨大。仪器小型化的研究正在进行中。3.制备了三种新型含氟荧光染料（改性BODIPY）。虽然这些染料具有亲油性、水溶性和高发光强度，但它们与蛋白质或DNA结合的研究正在进行中。

This project among Ibaraki University, University of Alabama at Birmingham, and Stanford University was started at 1994. The purpose of this project is thedevelopment of new automatic immunoassay system which removed human errors. Although the purpose was not accomplished perfectly, the semi-automatic system was performed.The concrete results were summarized as follows :1.The automation of hard-ware of ILISPOT-IDIP system by computer controllThe automatic adressing of the stage of the microscope using the combination of stepping motor and computer was accomplished. Although the image processing system is semi-sutomatic at this time, the automatic recognition between real spots and psuede spots was performed.Concequently, it is thought that the performance of the ILISPOT-IDIP system attains relatively high level as the automatic immunoassy system.2.The application of color center laser as the light source of ILISPOT system was thought effective for the enhancement of the ditecting limitaion of the spots. Hpwever, the instrument of color center laser is so huge. The investigation of the down-sizing of the instrument now in progress.3.Three kinds of new fluoorine-containing fluorescent dyes (modified BODIPY) was prepared. Although the dyes have lipophilicity, solubility in water and high intensity of emmition, the study of the combination of the dyes with proteins or DNA is now in progress.