Fundamental of optogalvanic-Stark laser spectroscopy and its application to cell biology.

本研究是光电激光光谱学和激光斯塔克光谱学在细胞生物学中的应用之一。在强电场作用下，像里德伯能级这样的高位激发能级分散成几个能级。如果用可调谐激光泵浦将亚稳态激发到其中一个分散能级，则可以观察到不同波长的不同发射光谱。利用这些光谱学，人们可以期望给细胞一个理想的变化，并观察到特定的荧光发射，以检测特定的细胞或治疗癌细胞。我们的目标是通过这种方法从He-Cd^+白光激光中获得可调谐的波长，并通过切割癌症基因将其应用于癌症治疗。应用这种激光光谱技术，可以比使用散射模式的细胞分选仪更准确地检测细胞。然而，由于我们还没有细胞治疗的直接实验系统，为了在这个问题上取得最终目标，还需要几年的时间。我们的概念的最后检查将进入另一个并行运行的国际项目的手中。

This investigation is of one of the applications of optogalvanic laser spectroscopy and of laser Stark spectroscopy to the cell biology.Under strong electric field, high lying excited level like Rydberg level is dispersed into several levels. If an excitation from a metastable state to one of these dispersed levels is carried out by tunable laser pumping, various emission spectra i different wavelength would be observed.Using these spectroscopy, one can expect to give cells a desirable change and observe a specific fluorescence emission for detection of specific cell or for therapy of cancer cells.Our aim has been in getting tunable wavelength from He-Cd^+ white-light laser by this means and apply it to cancer treatment by cutting the cancer gene only. Applying such laser spectroscopy, it would be expected that accurater detection of cells than that be cell sorter which uses scattering pattern can be done.For getting final goal on this subject, however, several years are still necessary since we have no direct experimental system for cell treatment. Final check of our concept would be into the hands of another internatioonal project running in parallel.