Prevention of cattle-botulism in Australia

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The type C and D botulinum strains were isolated from the ground of a cattle farm (a pasture) in Australia. The type C toxin was purified from one type C culture, and its antigenicity was analyzed by monoclonal antibodies. Based on the results of antigenicity and toxin-neutralization tests, it was concluded that the C-terminal of the toxin can be used as effective vaccine.The peptide (15 amino acid residues) which is conserved in the light chain components of any different types of botulinum toxins was synthesized, and the monoclonal antibodies against it were prepared in rats. Though two monoclonal antibodies reacting with any types of toxins were obtained, they did not neutralize the toxigenicity, indicating that this peptide can not be used as vaccine, but the monoclonal antibodies thus obtained can be used for diagnosis of botulism caused by any types of toxins.