Prevention of cattle-botulism in Australia

澳大利亚牛肉毒杆菌中毒的预防

• 批准号: 06044152
• 负责人: OGUMA Keiji
• 金额: $ 1.41万
• 依托单位: Okayama University, Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044152/
• 关键词: Botulinum toxin; Botulism; Vaccine; Serum therapy; ELISA; Toxin gene

The type C and D botulinum strains were isolated from the ground of a cattle farm (a pasture) in Australia. The type C toxin was purified from one type C culture, and its antigenicity was analyzed by monoclonal antibodies. Based on the results of antigenicity and toxin-neutralization tests, it was concluded that the C-terminal of the toxin can be used as effective vaccine.The peptide (15 amino acid residues) which is conserved in the light chain components of any different types of botulinum toxins was synthesized, and the monoclonal antibodies against it were prepared in rats. Though two monoclonal antibodies reacting with any types of toxins were obtained, they did not neutralize the toxigenicity, indicating that this peptide can not be used as vaccine, but the monoclonal antibodies thus obtained can be used for diagnosis of botulism caused by any types of toxins.

C型和D型肉毒杆菌是从澳大利亚一个养牛场(牧场)的地面上分离出来的。从一株C型培养物中提纯C型毒素，并用单抗分析其抗原性。根据抗原性和毒素中和试验的结果，认为该毒素的C端可以作为有效的疫苗。合成了不同类型肉毒毒素轻链成分中保守的多肽(15个氨基酸残基)，并在大鼠体内制备了针对该多肽的单抗。虽然获得了两株能与任何一种毒素反应的单抗，但不能中和其致毒活性，说明该多肽不能作为疫苗使用，但所获得的单抗可用于任何一种毒素引起的肉毒杆菌中毒的诊断。

项目成果

Oguma,K.,Y.Fujinga,K.Inoue: "Structure and function of Clostridium botulinum toxins" Microbiol.Immunol.39. 161-168 (1995)

Oguma，K.，Y.Fujinga，K.Inoue：“肉毒杆菌毒素的结构和功能”Microbiol.Immunol.39。

Oguma, K., Y.Fujinaga, K.Inoue: "Structure and function of Clostridium botulinum toxins" Microbiol.Immunol.39. 161-168 (1995)

Oguma，K.，Y.Fujinaga，K.Inoue：“肉毒杆菌毒素的结构和功能”Microbiol.Immunol.39。

小熊惠二: "オーストラリアにおける牛のボツリヌス中毒" 日本細菌学雑誌. 50(印刷中). (1995)

Keiji Oguma：“澳大利亚牛的肉毒杆菌中毒”，《日本细菌学杂志》50（出版中）。

Oguma, K.: "Cattle-botulism in Australia." Japanese Journal of Bacteriology. 50 (in press). (1995)

Oguma, K.：“澳大利亚的牛肉毒杆菌中毒。”

Oguma,K,.Y.Fujinaga,K.Inoue,N.Fujii,N.Yokosawa,T.Watanabe,T.Ohyama,K.Takeshi,K.Inoue: "Molecular Approaches to Food Safety Issues Involving Toxic Microorganisms" Alaken,Inc,Colorado(印刷中), (1995)

Oguma, K,.Y.Fujinaga,K.Inoue,N.Fujii,N.Yokosawa,T.Watanabe,T.Ohyama,K.Takeshi,K.Inoue：“涉及有毒微生物的食品安全问题的分子方法”Alaken，公司，科罗拉多州（正在印刷中），（1995 年）