权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of absorption of Clostridium botulinum neurotoxin-nontoxic component complex from the small intestine.

肉毒杆菌神经毒素-无毒成分复合物从小肠吸收的分析。

基本信息

批准号：
10670256
负责人：
OGUMA Keiji
金额：
$ 2.05万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670256/
关键词：
Clostridium botulinum Botulism Botulinum toxin Hemagglutinin receptor sialic acid

项目摘要

Clostridium botulinum type A and C hemagglutinin positive progenitor toxins consist of three distinct components : neurotoxin (NTX), hemagglutinin (HA), and non-toxic non-HA (NTNH). The HA consists of four subcomponents designated HA1 (〜33kDa), 2 (〜17kDa), 3a (〜22kDa), and 3b (〜53kDa). Each HA subcomponent of types A and C was prepared as GST-fusion proteins. By employing purified HA-positive A and C toxins and GST-fusion proteins of each HA subcomponent, we found that the HA positive progenitor toxins, GST-HA1, and GST-HA3b of both A and C bound to human erythrocytes and microvilli of guinea pig upper small intestinal sections. In both A and C, GST-HA1bound via galactose moieties such as Gal- β1-4 GlcNAc, whereas GST-HA3b bound via sialic acid moieties. The HA positive type A toxin bound via galactose moieties, whereas type C bound via sialic acid moieties. Therefore, it was concluded that HA1 contributes to the type A toxin binding to the erythrocytes and microvilli, whereas HA3 contributes to the type C toxin binding. GST-HA2 and Gst-ha3a showed no detectable binding. On the binding test of A and C toxins to the glycolipids and glycoproteins extracted from the erythrocytes, type A toxin mainly bound glycophorin, whereas type C bound glycophorin and sialylparagloboside and GM3. Since glycophorin contains both sialylated and unsialylated branches of the sugar chains, type A toxin may bind to the unsialylated branch, whereas type C toxin binds to sialylated branch.

肉毒梭菌A型和C型血凝素阳性祖毒素由三种不同的组分组成：神经毒素（NTX）、血凝素（HA）和无毒非HA（NTNH）。HA由四个亚组分组成，分别命名为HA 1（约33 kDa）、2（约17 kDa）、3a（约22 kDa）和3b（约53 kDa）。将A型和C型的每个HA亚组分制备为GST融合蛋白。通过使用纯化的HA阳性A和C毒素以及每个HA亚组分的GST融合蛋白，我们发现A和C的HA阳性祖毒素GST-HA 1和GST-HA 3b与人红细胞和豚鼠小肠上段微绒毛结合。在A和C中，GST-HA 1通过半乳糖部分如Gal- β1-4 GlcNAc结合，而GST-HA 3b通过唾液酸部分结合。HA阳性A型毒素通过半乳糖部分结合，而C型通过唾液酸部分结合。因此，可以得出结论，HA 1有助于A型毒素结合红细胞和微绒毛，而HA 3有助于C型毒素结合。GST-HA 2和Gst-ha 3a未显示可检测的结合。在A和C毒素与从红细胞提取的糖脂和糖蛋白的结合试验中，A型毒素主要结合血型糖蛋白，而C型毒素结合血型糖蛋白、唾液酸副糖甙和GM 3。由于血型糖蛋白含有糖链的唾液酸化和非唾液酸化分支，A型毒素可结合至非唾液酸化分支，而C型毒素结合至唾液酸化分支。