3-D digital morphology of neurons and glia cells

神经元和神经胶质细胞的 3D 数字形态

• 批准号: 06044237
• 负责人: HAMA Kiyoshi
• 金额: $ 10.3万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044237/
• 关键词: 3-D morphometry; tomography; HVEM; CFLSM; dissector; dendritic spine; astrocyte; synaptogenesis; Ca imaging; ディジタル構造解析; 連続切片再構築

The aim of the present project is to develop the methods of 3-D morphometrical analysis of the CNS utilizing the digitized structural images acquired by means of light and electron optical techniques by the collaboration of leading researchers inside and outside of the country, and apply these techniques for the brain research.Hama, Arii, Ellisman group is assigned to remodel the HVEM system in NIPS for accurate computer tomography of thick biological specimens, and to develop computer technique to support this system. Now HVEM serial tomography system in NIPS is available for researchers inside and outside the country. This group also developed several new fluorescent dyes essential for the optical digital imaging.Murakami, Wilson group : They utilized facilities in NIPS and UCSD and analyzed the process of synapse formation between corticorubral terminals and ruburospinal neurons. It was concluded that spine-like dendritic protrusions may play a crucial role in synaptogenic interaction during the development of the CNS.Kosak, Wilson group : They developed the dissector method application for the quantitative analysis of chemically identified neurons in the CNS using confocal laser scanning microscopy (CFLSM). They also utilized HVEM in NIPS and quantitatively analyzed the dendritic spines in dorsal and ventral hippocampus.Ohtsuka, Habara, and Stell group : They utilized CFLSM in NIPS and analyzed the process of photo-pigment synthesis in the retina and calcium signaling in variety of exciting cells.Through this project an international center of HVEM computer tomography for biological materials is established in NIPS which is most accurate and reliable one in the world.

本项目的目的是与国内外领先的研究人员合作，利用光和电子光学技术获得的数字化结构图像，开发CNS的三维形态测量分析方法，并将这些技术应用于脑研究。Ellisman小组的任务是改造NIPS中的HVEM系统，以便对厚生物标本进行精确的计算机断层扫描，并开发支持该系统的计算机技术。目前，NIPS的HVEM系列层析成像系统可供国内外研究人员使用。Murakami，Wilson小组：他们利用NIPS和UCSD的设备，分析了皮质红核终末和红核脊髓神经元之间突触形成的过程。得出的结论是，棘状树突状突起可能发挥了至关重要的作用，在突触的相互作用，在发展过程中的cns.Kosak，威尔逊组：他们开发了解剖方法的应用，定量分析化学鉴定的神经元在中枢神经系统使用共聚焦激光扫描显微镜（CFLSM）。他们还在NIPS中使用HVEM并定量分析了背侧和腹侧海马中的树突棘。Ohtsuka，Habara和Stell组：他们在NIPS中使用CFLSM，并分析了照片的过程，通过本项目，在国内建立了一个最准确、最可靠的HVEM生物材料计算机断层成像国际中心世界上

项目成果

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福田，T.，Aika，Y.，Heizmann，C.W.

村上富士夫: "脳における情報伝達-ネトリンとセマフォリン" 蛋白質・核酸・酵素増刊. 42. 571-576 (1997)

Fujio Murakami：“大脑中的信息传输 - Netrin 和 Semaphorin”蛋白质、核酸和酶特别版。42. 571-576 (1997)

J.Physiol.(Lond). (in press). (1995)

J.Physiol.（伦敦）。

Schultz,K.,Goldman,D.J.,Ohtsuka,T.,Hirano,J.,& Stell,W.K.: "Identification and localization of an immunoreactive AMPA-type glutamate receptor subunit (GluR4) with respect to identified photoreceptor synapses in the outer plexiform layer of goldfish retina

舒尔茨，K.，高盛，D.J.，大冢，T.，平野，J.，

Taomoto,K.Ijichi,A.,Arii,T.& Hama,K.: "Changes of intermediate filaments in cultured human glioma cells with various growth factors andcytokines using high voltage immuno-electron microscopy." Brain Tumor Pathology. 13. 49-56 (1996)

陶本，K.Ijichi，A.，Arii，T.