Clinicopathological study on the presence of dendritic cells and the expression of oncogene products in malignant tumors

恶性肿瘤中树突状细胞的存在及癌基因产物表达的临床病理学研究

• 批准号: 06045037
• 负责人: OHTSUKI Yuji
• 金额: $ 2.88万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06045037/
• 关键词: S-100 protein; P53 protein; Immunohistochemistry; Esophageal cancer; Urinary bladder cancer; Pelvic & ureteral cancer; Skin cancer; Sarcoma; 樹状細胞; 骨肉腫; サイクリン; 腎盂尿管癌; 咽頭癌; 肝癌

Clinicopathological study on the presence of S-100 protein positive dendritic cells and the expressions of oncogene products, such as p53, Rb, MDM-2, bcl-2, has been made immunohistochemically using both Japanese and Chinese patients with cancer. The kinds of carcinomas investigated were skin, liver, urinary bladder, esophagus, renal pelvis, ureter and larynx, and some sarcomas. As for S-100DC,these cells in increased numbers are a valuable marker for better prognosis in hepatocellular carcinoma, transitional cell carcinoma of urinary bladder and squamous cell carcinoma of esophagus. Similar results were obtained in Chinese patients with esophageal and urinary bladder carcinoma. p53 protein positivity of esophageal, renal pelvic and ureteral carcinoma showed the intimate relationship with poor prognosis statistically. Human papilloma virus (HPV) type 16 and 18 infection as well as overexpressed MDM-2, cyclin A and D,and Rb was also suggested the patient's poor prognosis. In addition, p53 or bcl-2 expression detected in the dysplastic lesions of esophagus, ureter, and renal pelvis suggested that these overexpressions might be an early event in carcinogenesis.Thus, the presence of S-100DC in increased numbers is the favorable marker, but HPV infection as well as overexpressed oncogene products or cyclins in cancer cells is the worse marker for the prognosis of cancer patients. As for S-100DC in sarcomas, especially osteosarcoma, there detected only few or no such kind of cells in the tumor tissues, as far as examined. These results may suggest the poor prognosis of these sarcoma patients, but further investigations are needed to clarify the exact meaning of these results.

应用免疫组织化学方法对日本和中国癌症患者中是否存在S-100蛋白阳性树突状细胞及其癌基因产物P53、Rb、mdm-2、bc1-2的表达进行了临床病理研究。调查的肿瘤种类有皮肤癌、肝癌、膀胱癌、食道癌、肾盂癌、输尿管癌和喉癌，以及部分肉瘤。对于S-100DC来说，这些细胞数量的增加是判断肝细胞癌、膀胱移行细胞癌和食管鳞癌预后较好的有价值的指标。在中国的食道癌和膀胱癌患者中也得到了类似的结果。P53蛋白在食管癌、肾癌、肾癌、输尿管癌中的表达与预后密切相关。人乳头瘤病毒(HPV)16型和18型感染以及MDM-2、细胞周期蛋白A、D和Rb的过度表达也提示患者预后不良。此外，在食道、输尿管和肾盂的异常增生性病变中检测到P53和bcl2的表达，提示这些过度表达可能是癌变的早期事件，因此，S-100DC的存在增多是有利的标志，而HPV感染以及癌细胞中癌基因产物或细胞周期蛋白的过度表达则是癌症患者预后的较差标志。而在肉瘤，尤其是骨肉瘤中，S-100DC仅在肿瘤组织中检测到极少数或未检测到此类细胞。这些结果可能表明这些肉瘤患者的预后很差，但需要进一步的研究来澄清这些结果的确切意义。

项目成果

石川忠則他: "食道癌におけるヒトパピローマウイルスと癌関連遺伝子" 消化器外科. 19. 1587-1594 (1996)

Tadanori Ishikawa 等人：“食管癌中的人乳头瘤病毒和癌症相关基因”胃肠外科。 19. 1587-1594 (1996)

H.Ono, et al.: "Relationship between H-ras product and p53 protein or high-risk human papillomaviruses in esophageal cancer from kochi, Japan" Am.J.Gastroenterol.89. 646-647 (1994)

H.Ono 等人：“日本高知县食道癌中 H-ras 产物与 p53 蛋白或高危人乳头瘤病毒之间的关系”Am.J.Gastroenterol.89。

M.Furihata, et al.: "The aberrant p53 protein (Review)" Int.J.Oncol.6. 1209-1226 (1995)

M.Furihata 等人：“异常的 p53 蛋白（综述）”Int.J.Oncol.6。

I.Yamasaki, et al.: "Overexpression of MDM2 and p53 protein is infrequently but significantly associated with progression of human proctatic adenocarcinoma" Oncology Report. 3. 925-929 (1996)

I.Yamasaki 等人：“MDM2 和 p53 蛋白的过度表达很少但与人类直肠腺癌的进展显着相关”肿瘤学报告。

M.Furihata, et al.: "Determination of the prognostic significance of unsheduled cyclin A overexpression in patients with esophageal squamous cell carcinoma" Clinical Cancer Research. 2. 1781-1785 (1996)

M.Furihata 等人：“确定食管鳞状细胞癌患者中未调度的细胞周期蛋白 A 过度表达的预后意义”临床癌症研究。