Clinicopathological study on the presence of dendritic cells and the expression of oncogene products in malignant tumors

应用免疫组化方法对日本和中国人肺癌患者的S-100蛋白阳性树突状细胞的存在和癌基因产物p53、Rb、MDM-2、bcl-2的表达进行了临床病理学研究。所调查的癌症种类包括皮肤癌、肝癌、膀胱癌、食道癌、肾盂癌、输尿管癌、喉癌和一些肉瘤。S-100 DC细胞数量增多是肝细胞癌、膀胱移行细胞癌和食管鳞状细胞癌预后较好的一个有价值的标志。在中国食管癌和膀胱癌患者中获得了类似的结果。食管癌、肾盂癌和输尿管癌p53蛋白阳性与预后不良密切相关。人乳头瘤病毒（HPV）16型和18型感染以及MDM-2、细胞周期蛋白A和D、Rb的过表达也提示患者预后不良。此外，在食管、输尿管和肾盂的异型增生病变中检测到p53或bcl-2的表达，提示这些过度表达可能是癌变的早期事件，因此，S-100 DC数量增加是有利的标志，而HPV感染以及癌细胞中癌基因产物或细胞周期蛋白的过度表达是癌症患者预后的不良标志。肉瘤尤其是骨肉瘤中S-100DC的表达很少或根本没有。这些结果可能表明这些肉瘤患者的预后不良，但需要进一步的调查，以澄清这些结果的确切含义。

Clinicopathological study on the presence of S-100 protein positive dendritic cells and the expressions of oncogene products, such as p53, Rb, MDM-2, bcl-2, has been made immunohistochemically using both Japanese and Chinese patients with cancer. The kinds of carcinomas investigated were skin, liver, urinary bladder, esophagus, renal pelvis, ureter and larynx, and some sarcomas. As for S-100DC,these cells in increased numbers are a valuable marker for better prognosis in hepatocellular carcinoma, transitional cell carcinoma of urinary bladder and squamous cell carcinoma of esophagus. Similar results were obtained in Chinese patients with esophageal and urinary bladder carcinoma. p53 protein positivity of esophageal, renal pelvic and ureteral carcinoma showed the intimate relationship with poor prognosis statistically. Human papilloma virus (HPV) type 16 and 18 infection as well as overexpressed MDM-2, cyclin A and D,and Rb was also suggested the patient's poor prognosis. In addition, p53 or bcl-2 expression detected in the dysplastic lesions of esophagus, ureter, and renal pelvis suggested that these overexpressions might be an early event in carcinogenesis.Thus, the presence of S-100DC in increased numbers is the favorable marker, but HPV infection as well as overexpressed oncogene products or cyclins in cancer cells is the worse marker for the prognosis of cancer patients. As for S-100DC in sarcomas, especially osteosarcoma, there detected only few or no such kind of cells in the tumor tissues, as far as examined. These results may suggest the poor prognosis of these sarcoma patients, but further investigations are needed to clarify the exact meaning of these results.