Immunoelectron microscopic study on the expression of lymphoid markers in HTLV- producing cells

t细胞表面或t淋巴细胞上的t细胞受体(TCR)/CD3复合物是巨噬细胞识别抗原所必需的。通常，这些抗原呈现在主要组织相容性复合体（MHC）分子上。在这个识别系统中，TCR α链和β链的细胞外结构域被报道为抗原识别所必需的。另一方面，据报道，HTLV-I和-II的产生者（最初是t细胞）的抗原性在培养过程中发生变化，例如失去一些t细胞抗原或获得HLA-DR和/或CD25抗原，在高级别t细胞恶性肿瘤中也有报道。本研究通过免疫电镜观察，阐明了TCR/CD3复合物在人t淋巴细胞I型和ii型产生细胞表面的表达。WT-31 （TCR α/β）染色仅在PM中呈阳性，α f1 （TCR α）和β f1 （TCR β）染色仅在NM和rER中呈阳性。另一方面，CD3同时与NM/rER和PM发生反应。使用这些抗体，正常新鲜、新鲜和短期培养的HTLV-I载体淋巴细胞清楚地显示具有TCR/CD3复合物，特别是缺乏TCR α/β复合物，表明它们对抗原的识别存在缺陷。卡波西肉瘤相关疱疹病毒(KSHV)/人疱疹病毒-8 （HHV-8）的超微结构尚未完全阐明，尽管已有报道使用原发积液性淋巴瘤（PEL）细胞系KS-1发现没有eb病毒（EBV）合并感染。本研究在体外用12- o -tetradecanoy1- phorpol -13-acetate （TPA）刺激pel来源的细胞系KS-1后，对KSHV/HHV-8进行了详细的精细结构检查。而核病毒颗粒没有与细胞外成熟颗粒相关联。受TPA刺激的KS-1细胞产生了许多细胞外成熟颗粒和核内颗粒，除了有趣的管网状结构和囊泡中的聚集管状结构。诱导的核内颗粒为空心、甜甜圈状、致密核，外径为100 ~ 110nm，内径为60 ~ 70nm。胞外成熟颗粒直径150 ~ 160nm，核密集，部分核呈甜甜圈状，外径260nm的巨病毒为数不多。这些发现表明，经TPA处理的KS-1细胞可以产生细胞外成熟颗粒和核内颗粒，这些颗粒被证明是KSHV/HHV-8。少

The T-cell receptor (TCR)/CD3 complex on the surface or T-lymphcytes is required for recognition of the antigens presented by macrophages. Usually, such antigens are presented on Major Histocompatibility Complex (MHC) molecules. In this recognition system, extracellular domains of both TCR α and β chains are reported to be essential for antigen recognition.On the other hand, it has been reported that the antigenicities of HTLV-I and -II producers, which are initially T-cells, change during cultivation, for example loosing some T-cell antigens or obtaining HLA-DR and/or CD25 antigens, as also reported in cases of high grade T-cell malignancy.In the present study, immunoelectron microscopic observation was performed to clarify TCR/CD3 complex expression on the surface of human T-lymphotropic virus (HTLV) type I- and type II-producing cells. Staining with WT-31 (TCR α/β) was found to be positive only in PM, while staining for αF1 (TCR α) and for βF1 (TCR β) was positive only in NM and rER … More . CD3, on the other hand, reacts with both NM/rER and PM.Using these antibodies, normal fresh, and fresh and short-term cultured HTLV-I carrier lymphocytes were clearly shown to possess TCR/CD3 complexes, lacking TCR α/β complex in particular, suggesting that their recognition of antigens is defcctive.The ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 (HHV-8) has not yet been fully elucidated, although some findings have been reported using primary effusion lymphoma (PEL) cell lines, KS-1, harboring no Epstein-Barr virus (EBV) coinfection. In the present study, detailed fine structural examination of KSHV/HHV-8 was performed after stimulation of the PEL-derived cell line KS-1 with 12-O-tetradecanoy1-phorbol-13-acetate (TPA) in vitro. While nuclear virus particles associated with no extracellular mature particles. KS-1 cells stimulated with TPA produced many extracellular mature particles as well as intranuclear particles, in addition to interesting tubulo-reticular structures and aggregated tubular structures in vesicles. The induced intranuclear particles were empty, doughnut shaped, and dense cored, with outer, and inner diameters of 100-110nm and 60-70nm, respectively. Dense-cored extracellular mature particles were 150-160nm in diameter, and some contained doughnut-shaped cores, together with a few megaloviruses, 260nm in outer diameter. These findings indicate that KS-1 cells treated with TPA can produce extracellular mature particles as well as intranuclear particles, which were proven to be KSHV/HHV-8. Less