Co-operative Research on Differentiation and Functional Expression of Blood Cells in Ascidiar

海鞘血细胞分化及功能表达的合作研究

• 批准号: 06305008
• 负责人: MICHIBATA Hitoshi
• 金额: $ 11.26万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06305008/
• 关键词: Marine organisms; Ascidians; Alloreognition; Expression of functions; Accumulation of metals; Vanadium; Proteins; 金属イオン; 濃縮; ホヤの血球; 組織特異性; 金属濃縮機構

Ascidian blood cells, that can be classified morphologically into approximately 10 types, are of advantage to analyze various functions such as immune responses, the differentiation of progenitor cells into peripheral cells and the accumulation of heavy metal. We have studied in an integrated manner. Consequently, we obtained several type of monoclonal antibodies and polyclonal antibodies that react specifically with several types of blood cells. We found that glyco-proteins are involved in allogeneic interaction and phenoloxidase and protease inhibitors released from the blood cells participate in the so-called contact reaction. We first succeeded to isolate at least four different types of vanadium-binding proteins, approx. 100kDa, 40kDa, 15kDa, and 12.5kDa and to analyze amino acid sequences of them partially. Cell culture of hemoblasts was established.

海鞘血细胞在形态上可分为约10种类型，有利于分析各种功能，例如免疫反应、祖细胞分化为外周细胞以及重金属的积累。我们以综合的方式进行了研究。因此，我们获得了几种类型的单克隆抗体和多克隆抗体，它们与几种类型的血细胞发生特异性反应。我们发现糖蛋白参与同种异体相互作用，血细胞释放的酚氧化酶和蛋白酶抑制剂参与所谓的接触反应。我们首先成功分离出至少四种不同类型的钒结合蛋白，大约。 100kDa、40kDa、15kDa和12.5kDa并部分分析它们的氨基酸序列。建立了造血细胞的细胞培养物。

项目成果

Hitoshi Michibata: "The Mechanism of Accumulation of Vanadium by Ascidians : Some Progress towards an Understanding of this Unusual Phenomenon." Zool.Sci.(in press.). (1996)

Hitoshi Michibata：“海鞘积累钒的机制：理解这种不寻常现象的一些进展。”

Wuchiyama, J.and Michibata, H.: "Classification, based on autonomous fluorescence, of the blood cells of several ascidians that contain high levels of vanadium." Acta Zool.76. 51-55 (1995)

Wuchiyama, J. 和 Michibata, H.：“根据自主荧光对几种含有高浓度钒的海鞘的血细胞进行分类。”

Uyama, T., Moriyama, Y., Futai, M.and Michibata, H.: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian Ascidia sydneiensis samea." J.Exp.Zool.270. 148-154 (1994)

Uyama, T.、Moriyama, Y.、Futai, M. 和 Michibata, H.：“海鞘类海鞘 (Ascidia sydneiensis Samea) 的钒细胞中液泡型 H+ -ATP 酶的免疫学检测。”

Kanamori,K.: "Raman spectroscopic study of the vanadium and sulphate in blood cells homogenates of the ascidian,Ascidia gemmata." J.Mar.Biol.Ass.U.K,. 74. 279-286 (1994)

Kanamori,K.：“海鞘血细胞匀浆中钒和硫酸盐的拉曼光谱研究。”

Takahashi,H.: "Hemocyte aggregation in the solitary ascidian Halocynthia roretzi:plasma factors,magnesium ion,and Met-Lys-bradykinin induce the aggregation." Biol.Bull.,. 186. 247-253 (1994)

Takahashi,H.：“孤生海鞘 Halocynthia roretzi 中的血细胞聚集：血浆因子、镁离子和 Met-Lys-缓激肽诱导聚集。”