权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular biological and clinocopathological study on the thymuses and lymph nodes of patients with myasthenia gravis

重症肌无力患者胸腺和淋巴结的分子生物学和临床病理学研究

基本信息

批准号：
06404049
负责人：
YOSHITAKE Tsuyoshi
金额：
$ 15.68万
依托单位：
Saitama Madical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06404049/
关键词：
Myasthenia gravis Thymus Thmoma Lymph node Immunohistochemical stain RT-PCR Apoptosis Tenascin RT‐PCR tenascin

项目摘要

Several distinct immunohistochemical and molecular biological characeristics were shown using the thymic and lymph node tissues of patients with myasthenia gravis.1. Thymic lymphoid follicles were surrounded by a fine matrix fiber tenascin, but these charcteisti compartmentalized structure was not found in the corresponding lymph nodes. In these thymic lymphoid follicles, immunohistochemically, expression of Fas antigen and p53 protein (apoptosis reacted factors) were less frequent than that in the other region of the thymus. Furthermore, Several densely but scatterd stained bc1-2 (apoptosis-depressing factor) positive B cells were observed in the thymic lymphoid follicles, although there were mostly no positive B cells in the lymph nodes. Expression of the antigen and protein were also confirmed at mRNA level through analysis using RT-PCR method.2. Patients, who had several highly developed thymic lymphoid hyperplasia, have preoperatively maintained higher level of serum anti-Ach-R an … More tibody titer than that of patients with poorly developed hyperplasia or without it.3. Population of memory and non-memory B cells of expression of adhesion molecules (LFA,ICAM) of the lymphoid follicles were similary recognized in both the thymus and lymph node.4. Epitheital cells from the thymuses and thymomas were immunochemically shown using anti-CK-17mAB among several anti-cytokeratin mAB Series. In the thymus, only medullary epithelium was positively stained. In thymomas, about half of the cases possessed positive cells. CK-17 mRNA expression in these cases were also confirmed by RT-PCR method. Patients with thymomas possessing CK-17-positive cessl showed higher level of seurm anti-Ach-R antibody titer and more developing lymphoid follicles in the residual thymuses than those lacking positive cells.CK-17-positive cell thymomas were both polygonal and polygonal-spindle shaped epithelial cell thypes, but contrary to out expection, spindle shaped epithelial cell type thymoma contained no positve cells.These results indicate that some antibody producing B cells in the thymic lymphold follicles associated with myasthenia gravis could escape the entry of apoptosis. Thus, they might survive to produce auto-antibody. Thymomas containing CK-17-positive epithelial cells seem to have a promoting ability of thymic B cells to make auto-antibodies. Regarding epithelial cell differentiation toward subtype of thymomas, there were a discrepancy in the morphological and immunohistochemical observations. Less

重症肌无力患者胸腺和淋巴结组织的免疫组化和分子生物学特征有明显差异.胸腺淋巴滤泡被一种细小的基质纤维腱生蛋白所包围，但在相应的淋巴结中没有发现这种特征性的区室化结构。免疫组化结果显示，Fas抗原和p53蛋白（凋亡反应因子）在胸腺淋巴滤泡中的表达较胸腺其它区域少。胸腺淋巴滤泡内有少量散在的bc 1 -2阳性B细胞，淋巴结内大多数无阳性B细胞。通过RT-PCR方法分析，在mRNA水平证实了抗原和蛋白的表达.有胸腺淋巴组织增生的患者，术前血清抗Ach-R和 ...更多信息抗体滴度明显高于不伴增生或不伴增生的患者.胸腺和淋巴结中淋巴滤泡中表达粘附分子（LFA、ICAM）的记忆和非记忆B细胞群相似.胸腺和胸腺瘤的上皮细胞用几种抗细胞角蛋白单克隆抗体系列中的抗CK-17单克隆抗体进行免疫化学显示。在胸腺中，仅髓质上皮呈阳性染色。在胸腺瘤中，大约一半的病例具有阳性细胞。RT-PCR法检测CK-17 mRNA表达。CK-17阳性的胸腺瘤与阴性胸腺瘤相比，其抗Ach-R抗体滴度高，残留胸腺内淋巴滤泡发育多，CK-17阳性的胸腺瘤均为多边形和梭形上皮细胞，但与外界预期相反，梭形上皮细胞型胸腺瘤不含阳性细胞，提示重症肌无力胸腺淋巴滤泡内的某些抗体生成B细胞能逃避免疫球蛋白的进入，凋亡因此，它们可以存活以产生自身抗体。含有CK-17阳性上皮细胞的胸腺瘤似乎具有促进胸腺B细胞产生自身抗体的能力。关于上皮细胞向胸腺瘤亚型的分化，在形态学和免疫组化观察中存在差异。少