Structural Analysis of Interface between Dentin and Adhesive Restorative Materials

采用扫描电子显微镜（SEM）、透射电子显微镜（TEM）和共聚焦光学显微镜对粘接界面进行了形貌分析，其中扫描电子显微镜是最简单的方法。然而，扫描电镜有一个固有的缺点，即样品必须气相镀金，并在高真空条件下观察。由于脱水导致的样品损坏使得难以观察含水的粘合剂材料。最新开发的Environmental-SEM可在无金涂层和极低真空条件下使用。观察光固化玻璃聚烯烃水门汀修复体粘接界面的超微结构（Fuji II LC CAPUSULE和玻璃体），有或无表面处理（牙本质调理剂和CA-剂）。此外，采用银染色技术，在冷冻条件下观察牙本质-骨水泥界面的特征。光固化玻璃聚烯烃酸盐粘固剂在牙本质洞面上有一定的附着力，绝大多数牙本质洞面与光固化玻璃聚烯烃酸盐粘固剂的界面无间隙形成，脱钙牙本质表面有一透明薄层。在骨水泥-牙本质界面处观察到薄的骨水泥基质层，没有未反应的核。结果表明，骨水泥基质可覆盖脱钙牙本质的表层，但不能充分渗透到深层。在牙本质-粘固剂界面处未观察到诸如粘结树脂混合区的层。

Morphological analysis of the adhesive interface has been performed using the scanning electron microscope (SEM), transmission electron microscope (TEM) and confocal optical microscope, with the SEM being the simplest method among them. However, SEM has an inherent drawback that samples must be vapor coated with gold and observed under high vacuum condition. Sample damage due to dehydration makes observation difficult for water containing adhesive materials. Recently developed Environmental-SEM can be used without gold coating and extremely low vacuums condition. The purpose of this study was to observe ultrastructure of adhesive interfaces of light-cured restorative glass polyalkenoate cements (Fuji II LC CAPUSULE and vitremer) with or without surface treatment (Dentin Conditioner and CA-Agent) by using light microscopy, Cryo-SEM,and Environmental-SEM.Furthermore, the characteristics of the dentin-cement interface were examined by using a silver nitrate staining technique under the Cryo-SEM.Microscopic observation of sectioned specimens revealed that some segments of light-cured glass polyalkenoate cement were retained on the prepared dentin cavity floor without any gap formation at the interface in almost all specimens and a transparent thin layr was present on the superficial layr of the decalcified dentin. A thin cement matrix layr without unreacted core was observed at the cement-dentin interface. It was suggested that the cement matrix might impregnate the superficial layr of decalcified dentin, however, insufficient penetration into the deep layr was observed. A layr such as the hybrid zone for adhesive resin restorations was not observed at the cement-dentin interface.