Studies on Biomimetic Energy and Signal Transduction Systems

仿生能量与信号传导系统研究

• 批准号: 06453214
• 负责人: KOBUKE Yoshiaki
• 金额: $ 4.42万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06453214/
• 关键词: Artificial Ion Channel; Supramolecules; Bimolecular Channel; Macrocyclic Compounds; Photo Gating; Kalium Selectivity; Molecular Recognition; Voltage Dependence; マクロ双極子; アゾ化合物; 光異性化; 超分子; 分子認識; 平面脂質2分子膜

Ion channels transport metal cations such as Na^+, K^+, and Ca^<2+> or a Cl^- anion across the membrane with large fluxes and varying selectivities. These are fundamental molecular devices for biological signal transduction systems. Their functions were tried to be mimicked by totally synthetic organic molecules. The first approach employed supramolecular assembly formation of amphiphilic molecules in bilayr lipid membranes. Oligoether carboxylate and quaternary ammonium having two long alkyl chains were combined to make an amphiphilic ion pair. These types of molecules gave single channel currents with several conductivity levels. The observation was explained by assuming the formation of supramolecular channels in the membrane.When macrocyclic compounds were employed as the unit to define the channel mouth and membrane-inserting part was introduced by ion pairing or covalent bonds, bimolecular ion channels are expected to be formed by association of two molecules in the bilayr. By th … More is way, alpha-cyclodextrin, resorcin [4] arene, and calix [6] arene were modified by long acyl chains or methyl cholate. All these gave single ion channel currents with only one conductivity level for each channel, suggesting the successful control of the channel mouth or the assembly number. All these channels were cation selective, the selectivity factors being generally 5-6 for oligoether supramolecular channels and 15-30 for molecular channels having hydrophobic inner channel wall.When electric charges are not neutralized, e.g.a combination of phosphate and ammonium ions, voltage dependence of the open and closed times were observed.Macrodipoles induced by incorporation of charges in the membrane seem to be responsible for the voltage dependence. Alternative flux control was achieved by employing azo-compound as the hydrophobic component of the supramolecular channel unit. trans-Azo affoded single channel currents, while the photo-isomerized cis could not give any stable channel currents. Less

离子通道传输金属阳离子，如Na^+， K^+, Ca^<2+>或Cl^-阴离子在膜上具有大通量和不同的选择性。这些是生物信号转导系统的基本分子装置。他们试图用完全合成的有机分子来模仿它们的功能。第一种方法采用双膜脂质膜中两亲分子的超分子组装形成。羧酸低聚醚和具有两个长烷基链的季铵结合形成两亲性离子对。这些类型的分子提供了具有几个电导率水平的单通道电流。通过假设膜上形成了超分子通道来解释这一观察结果。当以大环化合物为单位定义通道口，并通过离子配对或共价键引入插膜部分时，双分子膜中的两个分子结合形成双分子离子通道。通过这种方法，α -环糊精、间苯二酚[6]芳烃和杯状[6]芳烃被长酰基链或甲基胆酸修饰。所有这些都给出了单个离子通道电流，每个通道只有一个电导率水平，表明成功控制了通道口或组装数。这些通道均具有阳离子选择性，低聚超分子通道的选择性因子一般为5-6，具有疏水内壁的分子通道的选择性因子一般为15-30。当电荷未中和时，例如磷酸盐和铵离子的结合，观察到打开和关闭时间的电压依赖性。膜中电荷的结合引起的大偶极子似乎是电压依赖性的原因。采用偶氮化合物作为超分子通道单元的疏水组分，实现了替代通量控制。反偶氮提供单通道电流，而光异构顺式不能提供稳定的通道电流。少

项目成果

Y.KOBUKE: "Towards Biophysics of Ion Channels" Antificial Ion Channels(印刷中), (1995)

Y.KOBUKE：“走向离子通道的生物物理学”反离子离子通道（印刷中），（1995）

Y.Tanaka, Y.Kobuke, and M.Sokabe: "Non-Peptidic Ion Channel with K+Selectivity" Angew.Chem.Int.Ed.Eng.34(6). 693-694 (1995)

Y.Tanaka、Y.Kobuke 和 M.Sokabe：“具有 K 选择性的非肽离子通道”Angew.Chem.Int.Ed.Eng.34(6)。

Y.Kobuke: "Advances in Supramclecular Chemistry,Vol.4" JAI Press(印刷中), (1997)

Y.Kobuke：“超分子化学进展，第 4 卷”JAI Press（正在印刷中），（1997 年）

Y.Kobuke, Y.Tanaka, and M.Sokabe: "Artificial Non-Peptide Ion Channels" Towards Biophysics of Ion Channels, Progress in Cell Research Series, M.Sokabe Ed., Elsevier, Amsterdam. Vol.6. 167-188 (1997)

Y.Kobuke、Y.Tanaka 和 M.Sokabe：走向离子通道生物物理学的“人工非肽离子通道”，细胞研究系列进展，M.Sokabe Ed.，Elsevier，阿姆斯特丹。

Y.Kobuke: "Artificial Models of Membrane Transport Proteins" Nihon Rinsho. 54(3). 161-167 (1996)

Y.Kobuke：“膜转运蛋白的人工模型”Nihon Rinsho。