CULTIVATION OF BROWN ROT FUNGI IN FOREST

森林中褐腐真菌的培养

基本信息

批准号：
06556027
负责人：
OGIYAMA Koichi
金额：
$ 2.82万
依托单位：
YAMAGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

In order to develope the edible mushroom cultivation with softwood medium, the following experiments were done ; (1) isolation of strains from wild mushroom fruit body of SUGI-HIRATAKE and MATSU-OZI, (2) classification of the strains by isozyme analysis, (3) larger scale production of strain in liquid medium by bio-reactor, (4) woody media cultivations in sugi wood powders containing rice brain, as well as branches, stumps or logs, (5) chemical analysis of the rot woods and (6) analysis of environmental condition on occurrence of fruit body.As the results, it was established that seven strains from respective mushroom species were isolated. Identification of strains was possible by esterase isozyme analysis of fruit body extractives. Larger scale production of seed hypha was completed by liquid culture using bi-oreactor. Experiments indicated that SUGI wood powders mixed with rice brain for SUGI-HIRATAKE and SUGI wood powders itself for MATSU-OZI were suitable as the media for the growth of mycelium. However formation of fruit body succeeded only for MATSU-OZI but unsuccessful for SUGI-HIRATAKE.In case of SUGI-HIRATAKE,primordia of fruit body occurred but did not grow to fruit body in the laboratory. On the other hand, the cultivation experiment using logs showed SUGI-HIRATAKE mycelium grow readily but growth of MATSU-OZI mycelium was inhibited by the contamination with Trichoderma etc. Fruit body formation of SUGI-HIRATAKE was unattainable in the laboratory but it attained at last when the logs, of which mycelium fully extended, were placed in the SUGI forest where wild SUGI-HIRATAKE occurred. Environment of SUGI forest was essential at present, especially moisture, temperature and irradiation at the ground surface for formation of SUGI-HIRATAKE mushroom.

为了用软木培养基开发可食用的蘑菇种植，进行了以下实验。（1）从sugi-hiratake和Matsu-ozi的野生蘑菇水果体中分离菌株，（2）通过同工酶分析对菌株进行分类，（（3）通过生物反应器中较大的液体菌株产生菌株，（4）（4）含有水稻大脑的Sugi木材培养物的木质培养基，包括水稻的分析，以及分支机构的分析（5）（5）（5）（5）（5）（5）（5）（5）（5）（5）（5）（5）（5）（6）在结果时发生，结果确定了各自蘑菇物种的七种菌株。通过酯酶同工酶分析果实人体萃取物可以鉴定菌株。使用双重反应器通过液体培养来完成种子菌丝的大规模生产。实验表明，Sugi木粉与水稻大脑的Sugi-Hiratake和Sugi Wood粉末粉与Matsu-ozi的粉末本身适合作为菌丝体生长的培养基。然而，果实的形成仅对寿命为毛质，但对sugi-hiratake的成功不成功。在sugi-hiratake的情况下，果实的原始生物发生在实验室中，但并未成长为水果体。另一方面，使用日志的耕作实验表明sugi-hiratake菌丝体很容易生长，但穆特苏菌菌丝体的增长受到对毛del虫的污染的抑制。果皮的污染等。Sugi-hiratake的果实体形成在实验室中是无法实现的，但在sucelium super frested sugi sugi sugi sugi the sugi sef sugi the sugi the sugi the sugi the sugie the sugi in n of sugi the sugi the sugi intherfe n of sugi ward the sugi。目前，Sugi森林的环境是必不可少的，尤其是在地面上的水分，温度和辐射，以形成Sugi-Hiratake蘑菇。