CHEIMICAL STUDY OF BIO-DEGRADATION OF FOLIAGE EXTRACTIVES IN FOREST

森林叶面提取物生物降解的化学研究

• 批准号: 06660202
• 负责人: OGIYAMA Koichi
• 金额: $ 1.34万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06660202/
• 关键词: brown-rot fungus; (-) kaurene; SUGI foliage extractives; ferruginol; bio-degradation; stem flow; water extractives; bio-remediation; (-)カウレン; バイオリメディエーション機能; スギ針葉; 非極性抽出物; 担子菌生長検定; 芝生・白菜生長検定; 水浸出物; pH変動; 酸性化; 発芽・成長試験; 菌糸成長試験; マツオ-ジ; スギ

To grasp the effect of foliage extractives to the withering of SUGI (Cryptomeria japonica D.Don.), the following items were investigated.(1) Fractionation and isolation of foliage extractives of SUGI and investigation of their plant growth regulating effects, (2) Change of diterpene hydrocarbone contents in foliages during drying under the natural condition, (3) Bio-degradation of ent-kaurene and ferruginol with brown-rot fungus, SUGI-HIRATAKE, (4) Investigation of pH,EC,UV absorption, content of inorganic ions and volatile organic compounds in the stem flow of SUGI tree, artificial water spray experiments and the water extractives of SUGI foliages.Extractives of SUGI foliage indicated the effects of promoting in cabbage seed growth and inhibiting in rye grass seed and brown rot fungus, SUGIHIRATAKE mycelium growthes. In regardless of surface sterilization, diterpene hydrocarbones in foliages increased in accopanied with moisture decrease rapidly but decreased readily after ten days. I … More t was confirmed that both of entkaurene and ferruginol were hardly degradaed with the action of SUGI-HIRATAKE in liquid media for a long time. Ferruginol yielded a trace of sugiol etc.in the culture medium. Remarkable difference of pH-values of the stem flows among several tree species were found in the late autamun. SUGI trees yielded stronger acidic stem streams in comparison with BUNA (Betula sp.) or MIZUNARA (Quercus sp) trees. As these phenomina were supported by the water spray experiments and water extraction of the foliages, the strong acidity of SUGI stem flow would be mainly due to the water extactives of SUGI foliage. Although attempt to identify the acidic compounds in inorganic ions, volatile organic compounds or ethyl acetate extractives in SUGI stem flows were unsuccessfull, it may be suggested that strong acidity in stem stream of SUGI would be a polar water soluble compound. The water leachings of SUGI foliage indicated the acidity in a early stage of extraction but turned to neutral pH after a long water soaking. Thus, the effect of tree components to the pH-values of natural water supply under the natural conditions such as shower, long rain, fluid or deep snows would be complex and varied depending on time, place and occasion but the strong acidic stem flow of SUGI tree would affect considerably to the forest soil and the ecosystem of SUGI forest. Less

为了掌握柳杉叶片提取物对柳杉枯萎的影响，调查了以下项目。(1)（2）自然干燥过程中叶片中二萜甾酮含量的变化;（3）褐腐菌SUGI-HIRATAKE对贝壳杉烯和铁桉酚的降解;（4）pH、EC、紫外吸收、杉木茎流中无机离子和挥发性有机物的含量，结果表明，苏吉叶水提物对甘蓝种子的生长有促进作用，对黑麦种子的生长有抑制作用，褐腐菌、杉平竹菌丝体生长。无论表面灭菌与否，叶片中二萜类黄酮含量随水分的增加而迅速下降，但10天后迅速下降。我 ...更多信息 结果表明，在液体介质中，杉平竹对贝壳杉烯和铁赤醇的降解作用时间较长，几乎不发生降解。在培养基中，铁醇产生了痕量的sugioletc.in不同树种树干液流pH值差异显著。SUGI树产生较强的酸性茎流相比，布纳（桦木属）。或MIZUNARA（Quercus sp）树。由于这些现象得到了喷水实验和叶片水提取的支持，所以SUGI茎流的强酸性主要是由于SUGI叶片的水提取物。虽然对SUGI茎流中的无机离子、挥发性有机物和乙酸乙酯萃取物中的酸性化合物的鉴定没有成功，但可以认为SUGI茎流中的强酸性可能是极性水溶性化合物。苏吉叶的水浸提液在浸提初期呈酸性，浸提时间长后pH值变为中性。因此，在阵雨、长时间降雨、流体或深雪等自然条件下，树木组分对天然供水pH值的影响是复杂的，并因时间、地点和场合而异，但苏吉树强酸性茎流对森林土壤和苏吉林生态系统的影响是显著的。少

项目成果

荻山紘一: "数種の樹幹流のpHと化学成分組成" 日本木材学会大会研究発表要旨集. 47. 487 (1997)

Koichi Ogiyama：“几种树干溪流的 pH 和化学成分”日本木材协会会议研究报告摘要集 47. 487 (1997)。

荻山紘一: "スギ針葉成分の芝生及び白菜種子生長に及ぼす影響" 日本林学会東北支部会誌. 47. 23-24 (1995)

Koichi Ogiyama：“雪松针成分对草和大白菜种子生长的影响”日本林业学会东北分会杂志 47. 23-24 (1995)。

Koichi OGIYAMA: "Effect of foliage extractives of Cryptomeria japonica to growths of rye grass and Chinese cabbage seed" J.Jpn.For.Soc.Tohoku Branch. 47. 23-24 (1995)

Koichi OGIYAMA：“柳杉叶提取物对黑麦草和大白菜种子生长的影响”J.Jpn.For.Soc.Tohoku Branch。

Yoshiyuki KUZIRAI: "Seasonal change of the plant growth regulators in the abnormal foliages of Cryptomeria japonica D.Don.var.nana" Abstracts of the 47th Annual Meeting of the Japan Wood Research Society, (Asakura Campus, Kochi-University, Kochi). 589 (19

Yoshiyuki KUZIRAI：“柳杉异常叶子中植物生长调节剂的季节变化”日本木材研究会第 47 届年会摘要，（高知大学朝仓校区，高知县）。

鯨井賢行: "枝変わりチャボスギの針葉中に含まれる植物生長調節物質の季節変動" 日本木材学会大会研究発表要旨集. 47. 589 (1997)

Takayuki Kujirai：“日本雪松树枝变化中所含植物生长调节剂的季节性波动”日本木材协会会议研究报告摘要 47. 589 (1997)。