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Development and Applications of biomedical probe microscopes

生物医学探针显微镜的研制及应用

基本信息

批准号：
06557001
负责人：
ABE Kazuhiro
金额：
$ 11.26万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

We produced probe microscopes for use for biomedical specimens and studied their applications. We produced the following 4 kinds of probe microscopes and decided many measuring conditions ; 1) conventional probe microscope to measure atomic force between the tip of the probe and the surface of the specimens, elasticity and viscosity of the specimens, friction force of the specimens and so on by contact, noncontact, and cyclic contact modes. 2) probe microscope to measure the specimens in liquid condition such as cultured cells in the cultured medium, 3) vacuum probe microscope to improve resolution power by decreasing a resistance due to air when measuring by the cyclic contact mode, and 4) scanning nearfied optimal atomic force microscope to measure optimal information of the specimens by laser transmission and fluorescence due to laser. Using these probe microscopes, we measured the topography of the surfaces of the tectorial membrane in the inner ear, cultured nerve cells, cultured glial cells, cultured fibroblasts, and spermatozoa and obtained fine topographical information of the specimens, cell membrane, and substance on the cell membrane, and information of cytoplasmic filaments just under the cell membrane, information of visco-elasticity of the cell surface, and micro-movements of cell membrance of the living cells. It is important for probe-microscopy tightly to fix the specimens on the surface of the specimen stage to prevent passive movement during the scanning of the probe.

我们生产了用于生物医学标本的探针显微镜，并研究了它们的应用。我们生产了以下4种探针显微镜，并确定了多种测量条件；1)常规探针显微镜通过接触、非接触、循环接触等方式测量探针尖端与试样表面之间的原子力、试样的弹性和粘度、试样的摩擦力等。2)探针显微镜用于测量液体条件下的标本，如培养基中的培养细胞；3)真空探针显微镜通过循环接触方式测量时减少空气阻力来提高分辨率；4)扫描近距离优化原子力显微镜通过激光透射和激光荧光来测量样品的最佳信息。利用这些探针显微镜，我们测量了内耳被膜、培养的神经细胞、培养的胶质细胞、培养的成纤维细胞和精子表面的地形，获得了标本、细胞膜和细胞膜上物质的精细地形信息，以及细胞膜下的细胞质丝的信息，细胞表面的粘弹性信息，以及活细胞细胞膜的微运动信息。在探针显微镜中，将样品紧密固定在试样台表面，以防止探针扫描过程中的被动运动是非常重要的。