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Micromorphological approaches to bone resorption and formation

骨吸收和形成的微形态学方法

基本信息

批准号：
07457575
负责人：
ABE Kazuhiro
金额：
$ 1.09万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

The mouse parietal bone, femur and tibia were examined mainly by scanning electron microscopy (SEM). Osteoblasts, osteocytes, and osteoclasts were exposed for SEM by our EDTA-KOH method to dissolve the bone matrix. The surfaces of the bone matrix or osteoid were exposed by a NaOCl maceration method or water jet. Some of parietal bones were examined by a back scattered method. Femurs and tibias were also examined after estrogen injections or cutting nerves for lower extremities. The results indicate or suggest the followings. 1) Osteocytes and osteoblasts are functionally different cells and differentiated from common osteogenic precursor cells. Osteocytes appears in the layr of osteoblasts. 2) Osteoblasts elongate many, parallel, long and thin cytoplasmic processes in the narrow spaces between the undersurface of the osteoblastic layr and the surface of the osteoid to form parallel narrow gaps for collagen fiber production. Thus, the collagen fibers are produced running parallel for the bone matrix. 3) Osteocytes are buried into the bone matrix by pulling by cytoplasmic processes of osteocytes and form meshwork to detect the stress loading to the bone and inform this information of stress to osteoblasts through the cytoplasmic processes attaching to the osteoblast processes. 4) Osteocytes inform the thickness of the forming bone lamella to the local osteoblasts. 5) Osteoclasts show 2 kinds of ruffled borders of different functional activities. Osteoclasts dissolve the bone matrix in 2 kinds of functional phases. 6) Bone formation and resorption are functionally coupled. 7) Back scattered images of the bone surfaces demonstrate the various stages of calcification of collagen fibers for the bone matrix.

用扫描电镜观察了小鼠顶骨、股骨和胫骨。通过我们的EDTA-KOH方法溶解骨基质，将成骨细胞、骨细胞和破骨细胞暴露于SEM。通过NaOCl浸渍法或水射流暴露骨基质或类骨质的表面。对部分顶骨进行背散射检查。在注射雌激素或切断下肢神经后，还检查了股骨和胫骨。结果表明或提示如下。1)骨细胞和成骨细胞是由成骨前体细胞分化而来的功能不同的细胞。成骨细胞层中出现骨细胞。2)成骨细胞在成骨细胞层下表面和类骨质表面之间的狭窄空间中拉长许多平行的长而薄的胞质突起，以形成用于胶原纤维产生的平行狭窄间隙。因此，产生的胶原纤维与骨基质平行。3)骨细胞通过骨细胞的胞质突起的牵拉而埋入骨基质中，并形成网状结构以检测骨的应力负荷，并通过附着于成骨细胞突起的胞质突起将应力信息告知成骨细胞。4)骨细胞向局部成骨细胞提供形成骨板的厚度。5)破骨细胞具有两种不同功能活动的皱褶边界。破骨细胞溶解骨基质分两种功能阶段。6)骨形成和骨吸收在功能上是耦合的。7)骨表面的背散射图像显示了骨基质胶原纤维钙化的各个阶段。