Studies on populotion dynamics of a perennial herb through DNA analysis and a matrix model

采用PCR方法对克隆植物小野草（Carex kobomugi）的一个地方群体的遗传结构进行了鉴定。本种原产于日本沙丘，通过根茎无性繁殖。每个品系的寿命为1年，平均每个品系产生1.2个子品系。在Ajigaura海岸沙丘系统野外获得了一个小枝（子科）种群的人口统计资料。在此基础上，建立了过渡矩阵模型，研究了黑子种群的生存和生长情况。实际人口的规模分布与稳态相似，这是模型所期望的。这表明种群处于接近稳定的状态。该模型预测，根据当地环境的变化，种群规模将以每年0.9-1.4个的速度增长。总DNA是从种子培养的单株植物中提取的。这些种子是从日本各地收集的。用PCR方法研究了群体间和群体内的DNA变异。结果表明，日本地方群体间的遗传变异非常有限。种群内遗传分化的可能性也被提出。

The PCR method was applied to identify genetic structure of a local population of a clonal plants, Carex kobomugi. The species is native to Japanese sand dunes and reproduce clonally by rhizomes. The longevity of each ramete is 1 year and each ramete produced on average 1.2 daughter rametes. Demographic data of a shoot (ramete) population was obtained in the field in Ajigaura coastal dune system. Based on the date, a transition matrix model was developed to investigate survival and growth of the ramete population. The size distribution of the real population was similar to that at a steady state, which was expected from the model. This implies that the population was under a near-stable condition. The model predicted that the size of the population will increase at a rate of 0.9-1.4/year depending on the variation in the local environments. Total DNA was extracted from individual plant raised from a seed. The seeds were collected from various localities in Japan. The DNA variations between and within populations were investigated by the PCR methods. The results showed that the genetic variation between local populations in Japan was very limited. A possibility of within-population genetic differentiation was also suggested.