Studies on populotion dynamics of a perennial herb through DNA analysis and a matrix model

通过DNA分析和矩阵模型研究多年生草本植物的种群动态

• 批准号: 06640827
• 负责人: KACHI Naoki
• 金额: $ 1.34万
• 依托单位: TOKYO METROPOLITAN UNIVERSITY (1995-1996)National Institute for Environmental Studies (1994)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06640827/
• 关键词: Sand dune; Carex Kobomugi; DNA analysis; demography; PCR method; matrix model; vegetative reproduction; clonal plant; Carex Kobomugi; マトリックスモデル; 栄養繁殖; DNAフィンガープリント

The PCR method was applied to identify genetic structure of a local population of a clonal plants, Carex kobomugi. The species is native to Japanese sand dunes and reproduce clonally by rhizomes. The longevity of each ramete is 1 year and each ramete produced on average 1.2 daughter rametes. Demographic data of a shoot (ramete) population was obtained in the field in Ajigaura coastal dune system. Based on the date, a transition matrix model was developed to investigate survival and growth of the ramete population. The size distribution of the real population was similar to that at a steady state, which was expected from the model. This implies that the population was under a near-stable condition. The model predicted that the size of the population will increase at a rate of 0.9-1.4/year depending on the variation in the local environments. Total DNA was extracted from individual plant raised from a seed. The seeds were collected from various localities in Japan. The DNA variations between and within populations were investigated by the PCR methods. The results showed that the genetic variation between local populations in Japan was very limited. A possibility of within-population genetic differentiation was also suggested.

将该方法应用于克隆植物Carex kobomugi地方群体的遗传结构鉴定。本种原产于日本沙丘，通过根状茎克隆繁殖。每株寿命为1年，平均每株产1.2株子株。野外采集了味古拉海岸沙丘系统嫩枝(株)种群的人口学数据。在此基础上，建立了研究分株种群存活和生长的转移矩阵模型。实际种群的大小分布与模型预期的稳定状态下的大小分布相似。这意味着种群处于接近稳定的状态。该模型预测，根据当地环境的变化，种群规模将以每年0.9-1.4的速度增长。从种子中培育出的单个植株中提取总DNA。这些种子是从日本各地收集的。用聚合酶链式反应方法研究了群体间和群体内的DNA变异。结果表明，日本地方群体间的遗传变异非常有限。还提出了种群内遗传分化的可能性。

项目成果

Ishikaw,S.et al.: "Shoot population dynamics of Carex kobomugi on a coastal sand dune in relation to its zonal distribution" Australian Journal of Botany. (in press). (1997)

Ishikaw,S.et al.：“拍摄沿海沙丘上的苔草种群动态及其区域分布”《澳大利亚植物学杂志》。

可知直毅・佐倉統・徳永幸彦・石川真一(藤井宏一編): "はじめてのえころじい" 裳華房, 195 (1995)

加智直树、佐仓修、德永幸彦、石川真一（藤井浩一编辑）：“我的第一个 Ekorojii” Shokabou，195（1995）

Ishikawa, S.and Kachi, N.: "Shoot population dynamics of Carex kobomugi on a coastal sand dune in relation to its zonal distribution." Australian Journal of Botany. (in press).(1997)

Ishikawa, S. 和 Kachi, N.：“拍摄沿海沙丘上的苔草种群动态与其带状分布的关系。”

Kachi, N.and Nishitani, S.: "Life-history evolution in herbaceous plants and an application of demography : review and perspective" Annual Report of Hokkaido Tokai Univ. Environmental Institute (1995). 55-61 (1996)

Kachi, N. 和 Nishitani, S.：“草本植物的生命史进化和人口统计学的应用：回顾和视角”北海道东海大学年度报告。

Nishitomi,S.,Takada,T.& Kachi,N.: "Optimal resource allocation to seeds and vegetative plopagules in an onderstory herb Syneilesis palmata (Compositae)" Plant Species Biology. 10. 127-135 (1995)

西富，S.，高田，T.