肝臓移植の拒絶反応における新しい免疫病理学的診断法の確立

肝移植排斥反应免疫病理诊断新方法的建立

• 批准号: 06770917
• 负责人: 中村 賢二郎
• 金额: $ 0.58万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06770917/
• 关键词: 肝臓移植; 拒絶反応; 免疫病理学; 小腸移植; 細胞間接着分子

1.研究の概要.肝臓移植の拒絶反応の評価で現在最も信頼されるのは,臨床的病態をふまえた移植肝組織の病理診断である.一般的な拒絶反応のみならず,移植直後から全く機能を示さない無機能肝や,治療にほとんど反応しない難治性拒絶反応のメカニズム解明のために細胞間接着分子の意義や従来の病理組織学的な拒絶反応診断との相関について検索している.2.本年度の実施結果および今後の展望(1)純系繁殖ラットを用い,無機能肝のモデルとして温阻血または冷阻血を施した群とそうでない群を作り,グラフトの生検肝組織を採取し,組織学的・免疫組織学的検討を行った.温阻血と冷阻血では肝臓の傷害部位やメカニズムに異なった因子が働いていることが組織学的に示唆され,生化学的・免疫組織化学的に解析中である.(2)肝へ流入する血流の大部分を占める門脈の支配領域である小腸に注目し,小腸移植に関しての組織学的研究を行った.小腸は保存や拒絶反応による傷害が他臓器に比べ高度であり,門脈を介して肝臓に多大な影響を与えるため今後研究の拡大が必要と考えられた.(3)肝移植や小腸移植に関して細胞間接着分子の意義を,臨床肝・小腸移植後の可溶性接着分子を中心に米国研究者とともに検討した.移植後,血中sICAM-1,sE-selectin,sIL-2,sHLA class Iは小腸移植群が肝・小腸同時移植群より高く,肝移植により小腸移植の拒絶反応の緩和に役立っていることを,接着分子やサイトカイン血中レベルから示した.

1. A summary of the study. The rejection of liver transplantation is now the most important. The pathological condition of the bed and the pathological changes of the transplanted liver tissue. In general, after transplantation, the whole machine can show that there is no chance of liver failure, and the treatment of anti-immune disease can explain the molecular meaning between the cells of the tumor, and then the molecular meaning between the cells of pathology. 2. The results of this year's results are as follows: (1) the future prospects of this year's results are as follows: (1) the main results of this year are as follows: (1) the results of this year's results are as follows: (1) the results of this year's results are as follows: (1) the results of this year's results are as follows: (1) the results of this year's results show that: (1) the future prospects of the results of this year's results are as follows: (1) the results of this year's results are as follows: (1) A series of reproductive drugs, such as warm blood resistance, cold blood resistance, liver tissue acquisition and immunology of histology. Warm blood stasis, cold blood blockage, liver injury, liver It is necessary to study the size of the liver in the future. (3) there is a molecular meaning between the cells of small liver transplantation, and the molecular meaning between the cells of liver transplantation. after bed liver transplantation, the molecular meaning is added to the molecular meaning of liver transplantation. after bed liver transplantation, the molecular meaning is added to the molecular level of liver transplantation. After transplantation, the sICAM-1,sE-selectin,sIL-2,sHLA class I small graft group in the blood and the liver transplantation group in the same time had a high level of liver transplantation, and the small liver transplant in the liver transplantation group refused to accept anti-graft therapy and in-service treatment, and then the molecular weight was detected.

项目成果

中村賢二郎、岩城裕一: "移植免疫 2.臓器移植の病理" 中山書店 現代病理学体系 第6巻、免疫, 12 (1994)

中村健二郎、岩木佑一：《移植免疫学2.器官移植的病理学》中山书店现代病理学系统第6卷，免疫学，12（1994）

Zeng Q-H.et al.: "Modified simple cold storage of rat livers with UW solution." Transplantation. 58. 408-414 (1994)

Zeng Q-H.et al.：“用 UW 溶液改良大鼠肝脏的简单冷藏。”

Nakamura K et al.: "Histologic evaluation of preservation injury in clinical small bowel transplantation" Transplantation Proceedings. 26. 1416 (1994)

Nakamura K 等人：“临床小肠移植中保存损伤的组织学评估”移植论文集。

Satoh S et al.: "Circulating adhesion moiecules and other soluble mavkers of immune activation in human small boivel and small bowel plus liver transplantation." Transplantation Proceedings. 26. 1417-1418 (1994)

Satoh S 等人：“人类小肠和小肠以及肝移植中免疫激活的循环粘附分子和其他可溶性标记物。”