損傷脊髄の再生におけるサイトカインと生物活性分子NOの機能に関する基礎的研究

细胞因子和生物活性分子NO在损伤脊髓再生中的作用基础研究

• 批准号: 06771067
• 负责人: 長谷川 光広
• 金额: $ 0.77万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06771067/
• 关键词: Spinal cord iniury; regenevation; NO

若年雌ウイスターラット(3-5週令)を用いて,損傷脊髄モデルならびに損傷脊髄の神経再生モデルを作成した。手術顕微鏡下に下位胸椎レベルに椎弓切除を加えたのちに硬膜越しに片側脊髄を圧挫し脊髄損傷を作成した(損傷モデル)。自家末梢神経移植組織として約10mm長の坐骨神経を取り出し,損傷部の上位,下位の灰白質にそれぞれ移植神経の上端下端を挿入し,脊髄損傷部位の中枢側と末梢側を架橋する組織とした(再生モデル)。両群ともに手術翌日(1日),3日,1,2,3,5,8,13週までの時期にパラフォルムアルデヒドで経心的に灌流固定したのち移植片を含む脊髄を取り出し,免疫組織化学的手法により,bFGF,IL-1の発現と局在を経時的に免疫電顕を含め詳細に検討した。細胞の同定に抗GFAP(反応性アストログリア),抗ED1(マイクログリア,マクロファージ),UCHL1(T cell),L-26(B cel),抗MHC class II(抗原提示細胞),抗S-100(Schwann細胞),抗ニューロフィラメント(再生軸票)を用いた。再生早期からbFGFが損傷周囲と移植組織に強陽性に検出され,損傷周囲と移植神経組織内に抗ED1陽性のマクロファージが多数集積していた。損傷モデルを損傷後1-日から慢性期に到るまでの期間で,麻酔導入後速やかに未固定のまま脊髄を損傷部を含めて摘出し,損傷部,中枢端,末梢端をそれぞれ10mmづつ凍結保存したのち,NOS活性を組織定量した.損傷部脊髄はコントロールとした正常脊髄に比べ4倍の活性を示した。現在も実験は継続中であり,解析総括ののち学会発表ならびに論文発表をする予定である。

If the female child (3-5 orders) is used, the spine will be reborn and made. In the hand, the lower thoracic vertebrae were removed, the lower thoracic vertebrae were removed and the dura mater was resected. In our own peripheral god transplantation organization, the 10mm ischial bone was removed, the upper part was superior, the lower part was gray, the upper and lower end of the transplant god was inserted, and the spinal cord was located in the central nervous system (regenerative organ). The following day (1st), 1st, 3rd, 1st, 3rd, 1st, 3rd, 1st, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd, 3rd The cells were identified as anti-GFAP, anti-ED1, anti-ED1, T-cell, Lmur26 (B-cel), anti-MHC class II (antigen-hint cell), anti-Smur100 (Schwann cell), anti-tumor antibody (regenerative ticket). In the early stage of bFGF regeneration, the transplant tissue was strongly stained, and the anti-ED1 antibody was detected in most of the transplant tissues. During the period from the chronic phase to the peak period after the first day of treatment, the part of the unfixed spinal cord contains the extract, the terminal, the central end and the end of the tip. during the period from the chronic phase to the chronic phase, the part of the unfixed spinal cord contains the extract, the end, the central end, and the end. The NOS active tissue was quantified. The normal spine is 4 times more active than that of the normal spine. Now in this section, we will analyze the information in the table of the Institute, the table of the text, the table of the predetermined information.