About influence of radiation for cultivation human skin fibroblasts and hormone

我研究了辐射、类固醇激素、甲状腺激素和辐射对人体皮肤培养成纤维细胞的影响。方法：对人皮肤培养成纤维细胞进行传代培养，用传代早期的α细胞进行模拟。射线照射能精确控制剂量，在线形下，选择了^<60>的Co γ射线，并在0.3Gy/min, 5Gy.10Gy下具有重复性。20Gy使用远距钴仪器。由于其他导线形式的剂量重现性不固定，所以我没有使用它进行模拟。我测量了72小时后的α细胞数量，并在24小时后立即暴露，评估细胞增殖能力以检查伽马射线暴露的影响，并在24小时后立即暴露时通过光学显微镜和扫描型/透射电子显微镜观察α细胞形态。在培养液中分别加入0.1、1.0、10.0 μ g/ml的甲状腺素（T4）和氢化肾上腺素（HC）， 0.7Gy/min，再用远距放射仪照射10Gy的伽马射线，在照射后即刻、24.48 h测量α细胞数量，评价细胞增殖能力，并在24 h后通过透射电镜观察α细胞形态。结果：人体皮肤培养成纤维细胞的增加受γ射线照射的抑制是公认的。光镜下未见α细胞形态学差异，但扫描电镜下观察微绒毛细胞表面的平滑及剂量增加。透射电镜下观察残体的增益和线粒体形态的变化，特别是24小时后低剂量观察，24小时后残体仍保持高剂量。在培养液中加入T4、HC，发现对人皮肤培养成纤维细胞的生长有抑制作用，而在HC中只照射γ射线。有一个决定是什么促进了T4细胞的增殖，人们认为你是否没有通过辐射减少屏障。问题：有些差异更多的是通过实验发现来认识到的，但对应的是使用同一年电荷，同一染色体区域进行原代培养的单个培养细胞的辐射敏感性差异。细胞的增殖能力受到辐射暴露的限制，但是因为在短期内观察到的变化并没有被识别出来，我认为当想要观察长期而不是未来时，我认为当想要做基因水平的分析时也更多。少

I examined influence of radiation, coaction of steroid hormone, thyroid hormone and radiation for human skin cultivation fibroblasts.Method : I did culture passage of human skin cultivation fibroblast, and used alpha cell of passage early stage for simulation. The radiation exposure could control dose precisely, and, in wire form, I selected^<60> Co gamma-ray by it ising possible for constant, and did reproducibllity with 0.3Gy/min, 5Gy.10Gy.20Gy using telecobalt apparatus. Because reproducibility of dose wasn't fixed in other wire form, I didn't use it for simulation. I measured alpha cell number after 72 hours and, after exposure right after, 24 hours, evaluated proliferation of cells ability to examine gamma-ray exposure influence, and observed alpha cell morphology by light microscope and scanning type/transmission electron microscope in exposure right after, after 24 hours.Next, 0.1,1.0,10.0 mu g/ml each added Thyroxine (T4) and Hydrocortison (HC) in culture medium, and 0.7Gy/min, … More 10Gy irradiated gamma-ray using telecobalt apparatus, and I measured alpha cell number after exposure right after, 24.48 time and evaluated proliferation of cells ability, and observed alpha cell morphology by transmission electron microscope in after 24 hours.Consequence : It was accepted an increase of human being cutis cultivation fibroblast to be restrained by gamma-ray exposure. About alpha cell morhology, the differential wasn't found with light microscope, but smoothing of decrement and cell surface of microvilli was looked at with gain of dose with scanning electron microscope. And, in transmission electron microscope, alteration of morphology of gain and mitochondria of residual body was looked at, and, in particular, was looked at with low dose by after 24 hours, and residual body remained with high dose even after 24 hours.That growth of human being cutis cultivation fibroblast was restrained was recognized by adding T4, HC in culture medium than I irradiated a merely gamma-ray in HC too. And, there was the determination which promoted proliferation of cells in T4, and it was thought whether you didn't reduce barrier by radiation exposure.Problems : Some differences are recognized more by experimental findings to boil it, but correspond to a difference of radiation sensitivity of individual cultured cell by using the same year charges, the same chromosome region for primary culture. Proliferation of cells ability is restrained by radiation exposure, but because the alteration which was wasn't recognized in observation between short terms, I think about alpha cell morphology when want to observe a long term than the future, and think when want to do analysis by gene level more too. Less