About influence of radiation for cultivation human skin fibroblasts and hormone

辐射对培养人皮肤成纤维细胞及激素的影响

• 批准号: 06671474
• 负责人: NARA Taku
• 金额: $ 1.41万
• 依托单位: Iwate medical university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06671474/
• 关键词: Cultivation; Fibroblast; Radiation; Thyroid hormone; Steroid hormone

I examined influence of radiation, coaction of steroid hormone, thyroid hormone and radiation for human skin cultivation fibroblasts.Method : I did culture passage of human skin cultivation fibroblast, and used alpha cell of passage early stage for simulation. The radiation exposure could control dose precisely, and, in wire form, I selected^<60> Co gamma-ray by it ising possible for constant, and did reproducibllity with 0.3Gy/min, 5Gy.10Gy.20Gy using telecobalt apparatus. Because reproducibility of dose wasn't fixed in other wire form, I didn't use it for simulation. I measured alpha cell number after 72 hours and, after exposure right after, 24 hours, evaluated proliferation of cells ability to examine gamma-ray exposure influence, and observed alpha cell morphology by light microscope and scanning type/transmission electron microscope in exposure right after, after 24 hours.Next, 0.1,1.0,10.0 mu g/ml each added Thyroxine (T4) and Hydrocortison (HC) in culture medium, and 0.7Gy/min, … More 10Gy irradiated gamma-ray using telecobalt apparatus, and I measured alpha cell number after exposure right after, 24.48 time and evaluated proliferation of cells ability, and observed alpha cell morphology by transmission electron microscope in after 24 hours.Consequence : It was accepted an increase of human being cutis cultivation fibroblast to be restrained by gamma-ray exposure. About alpha cell morhology, the differential wasn't found with light microscope, but smoothing of decrement and cell surface of microvilli was looked at with gain of dose with scanning electron microscope. And, in transmission electron microscope, alteration of morphology of gain and mitochondria of residual body was looked at, and, in particular, was looked at with low dose by after 24 hours, and residual body remained with high dose even after 24 hours.That growth of human being cutis cultivation fibroblast was restrained was recognized by adding T4, HC in culture medium than I irradiated a merely gamma-ray in HC too. And, there was the determination which promoted proliferation of cells in T4, and it was thought whether you didn't reduce barrier by radiation exposure.Problems : Some differences are recognized more by experimental findings to boil it, but correspond to a difference of radiation sensitivity of individual cultured cell by using the same year charges, the same chromosome region for primary culture. Proliferation of cells ability is restrained by radiation exposure, but because the alteration which was wasn't recognized in observation between short terms, I think about alpha cell morphology when want to observe a long term than the future, and think when want to do analysis by gene level more too. Less

方法：将人皮肤培养成纤维细胞进行传代培养，用传代早期的α细胞进行模拟。射线照射量可精确控制剂量，在线状照射中，我选择<60>了γ射线，使其恒定，并用远距离钴仪进行了0.3Gy/min、5Gy、10 Gy、20 Gy的重复性试验。因为剂量的可重复性在其他导线形式中不固定，所以我没有将其用于模拟。在72小时后测量α细胞数量，在暴露后即刻，24小时后，评价细胞增殖能力以检查γ射线暴露影响，并在暴露后即刻，24小时后，通过光学显微镜和扫描型/透射电子显微镜观察α细胞形态。培养基中加入甲状腺素（T_4）和氢化可的松（HC）各10.0 μ g/ml，0.7Gy/min， ...更多信息 10 Gy γ射线照射后即刻、24.48倍测定α细胞数，评价细胞增殖能力，24小时后用透射电镜观察α细胞形态，结果：人皮肤培养成纤维细胞的增殖受γ射线照射抑制。α细胞形态学观察，光镜下未见差异，扫描电镜下可见随剂量增加，细胞表面微绒毛减少，细胞表面光滑。透射电镜下观察到细胞生长和线粒体的形态学变化，特别是低剂量照射24小时后可见细胞生长和线粒体的形态学变化，高剂量照射24小时后仍可见细胞的形态学变化，在培养液中加入T_4、HC比单纯照射HC对人皮肤成纤维细胞的生长有抑制作用。而且，有促进细胞增殖的T4的决心，并认为你是否没有减少辐射暴露的障碍。问题：一些差异被认为是更多的实验结果煮它，但对应于不同的辐射敏感性个别培养细胞通过使用相同的一年收费，相同的染色体区域的原代培养。细胞的增殖能力受到辐射照射的抑制，但由于在短期间的观察中没有认识到这种变化，所以当想要观察长期时，我会考虑α细胞的形态，而不是将来，并且当想要从基因水平进行分析时，我也会考虑更多。少

项目成果

袖井文二: "培養ヒト皮膚線維芽細胞に対するホルモンと放射線の影響" 岩手医学雑誌. 48・4. 483-499 (1996)

Bunji Sodei：“激素和辐射对培养的人类皮肤成纤维细胞的影响”岩手医学杂志 48・4 483-499（1996）。