The Distribution of Nerve Endings in the sheath of Human Cervical Nerve Roots, with a Focus on Free Nerve Endings

人体颈神经根鞘神经末梢的分布，以游离神经末梢为重点

• 批准号: 06671479
• 负责人: TAKAHASHI Kuniyasu
• 金额: $ 1.34万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06671479/
• 关键词: Cervical nerve root; Dorsal root ganglion; Sensory nerve ending; Cervical radicular pain; 疼痛; 自由神経終末; 神経終末; 神経鞘

The presence and distribution of nerve endings in the dural sheath of cervical nerve roots (dural root-sheaths) was investigated histopathologically using 39 cervical root-dorsal root ganglion specimens obtained from 18 postmortem examinations within 12 hours after death. Ages ranged from 4 months to 88 years, with a mean of 58 years. Microscopic investigation was conducted using 20 mum serial sections of various parts of the dural root-sheath, stained by the Suzuki silver impregnation method or Kluver-Barrera method. Free nerve endings were identified in each of the dural root-sheaths of the anterior root, posterior root and dorsal root ganglia (DRG), including the septum, and two types of sensory corpuscles were identified in each of the dural root-sheaths of the dorsal root ganglia and the septum. The fiber diameters of free nerve endings corresponded to those of either Adelta-fibers or C-fibers. The distribution of free nerve endings was greater in the proximal and distal portions of the DRG sheath than in other parts of the dural root-sheath. The largest population of free nerve endings with C-fibers in the DRG sheath was 12 in a tissue cylinder with a volume of 0.01 mm^3 (0.16mm^2*0.06mm), with an average of 3.7. These nerve endings were found to follow either of two centripetal routes, one through a sinuvertebral nerve and the other through small nerves which penetrated directly into the DRG from nerve endings. The number of free nerve endings in the dural root-sheath increased with ages (gamma=0.75). In addition, the number of free nerve endings in subjects below the age of sixty was lower than that of subjects above sixty with a statistical significance (p=0.019). These free nerve endings in the dural root-sheath, especially those in the DRG sheath, are thought to play an important role in the mediation of cervical radicular pain.

使用死后12小时内18次尸检中获得的39个颈根-背根神经节标本，对颈神经根硬脑膜鞘（硬脑膜根鞘）中神经末梢的存在和分布进行组织病理学研究。年龄从4个月到88岁不等，平均58岁。使用硬脑膜根鞘不同部分的20μm连续切片进行显微镜检查，通过铃木银浸渍法或克鲁弗-巴雷拉法染色。在前根、后根和背根神经节（DRG）的每个硬脑膜根鞘（包括隔膜）中识别出游离神经末梢，并且在背根神经节和隔膜的每个硬脑膜根鞘中识别出两种类型的感觉小体。游离神经末梢的纤维直径与 Adelta 纤维或 C 纤维的直径相对应。游离神经末梢在 DRG 鞘的近端和远端部分的分布比硬脑膜根鞘的其他部分更大。在体积为0.01 mm^3（0.16mm^2*0.06mm）的组织筒中，DRG鞘中含有C纤维的游离神经末梢数量最多为12个，平均为3.7个。发现这些神经末梢遵循两种向心路线之一，一种通过窦椎神经，另一种通过从神经末梢直接穿透到背根神经节的小神经。硬脑膜根鞘中游离神经末梢的数量随着年龄的增长而增加（gamma=0.75）。此外，60岁以下受试者的游离神经末梢数量低于60岁以上受试者，具有统计学意义（p=0.019）。硬脑膜根鞘中的这些游离神经末梢，尤其是 DRG 鞘中的游离神经末梢，被认为在颈神经根性疼痛的调节中发挥重要作用。