Molecular and Cellular Biology of Tobacco BY-2 Cell Line

烟草 BY-2 细胞系的分子和细胞生物学

• 批准号: 07304053
• 负责人: NAGATA Toshiyuki
• 金额: $ 7.3万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07304053/
• 关键词: tobacco BY-2 cells; cell synchronization; cytoskeletons; auxin; cytokinin; in vitro transcription system; miniprotoplast; 細胞増殖; インビトロ翻訳系; 植物ホルモン

This project has been intended and conducted to facilitate the acceleration of research progress and information change on the study of molecular and cellular biology of tobacco BY-2 cell line. This subject is quite unique, since this is only one available highly synchronized system in the world that has been developed by the principal investigator and has been distributed to more than 100 laboratories in at least 20 countries. This is indispensable in particular for the cell cycle studies and is being used in major laboratories in the world. Thus' it was intended in this study to facilitate the informational exchange and romote the progress of scientific studies using this cell line in this Country as well.As aconsequence after two years' efforts, it was shown by using highly synchronized cell populations that molecular mechanism of the organization of cortical microtubules has been clearly demonstrated and several types of kinesin molecules oscillated differently during cell cycle pr … More ogression. It is also revealed that a novel auxin-signal transduction chain is involved in the maintanance of cell division activity in tobacco BY-2cells. Furthermore, the development of a novel in vitro transcription system by one of collaborators using BY-2 cells facilitated further the popularity of this cell line at the international level. It was also shown that there are two different transport systems of proteins into vacuoles that are dependent on unique sequence in the C-terminal and N-terminal sequences of proteins, respectively. Conversion of proplastids to amyloplasts upon the treatment by cytokinin has also been analyzed by one investigator. Thus, it may be said that even within the short frame of two years significant progress of the understanding of the cellular and molecular biology on tobacco BY-2 has been attained, resulting in understanding the basic phenomenon of plant cells in general.In 1995, a public forum was held at the occasion of Annual Meeting of the Botanical Society of Japan to familiarize the topics of discussion in this group to much wider circles in this country. Responses from the audiences were quite significant, since some of these peoples would like to introduce this experimental system in their studies. Less

本项目旨在促进烟草BY-2细胞系分子和细胞生物学研究的进展和信息的更新。这是一个非常独特的课题，因为这是世界上唯一一个由首席研究员开发的高度同步的系统，并已分发给至少20个国家的100多个实验室。这是不可或缺的，特别是对细胞周期的研究，并正在使用在世界上的主要实验室。因此，本研究旨在促进信息交流，促进我国利用该细胞系的科学研究进展。因此，经过两年的努力，通过高度同步化的细胞群体，已经清楚地表明了皮层微管组织的分子机制，并且几种类型的驱动蛋白分子在细胞周期过程中有不同的振荡。 ...更多信息 表情研究还表明，一条新的生长素信号转导链参与了烟草BY-2细胞分裂活性的维持。此外，合作者之一使用BY-2细胞开发了一种新的体外转录系统，进一步促进了该细胞系在国际上的普及。研究还表明，有两种不同的运输系统的蛋白质进入液泡，这是依赖于独特的序列中的C-末端和N-末端的蛋白质序列，分别。一位研究者还分析了细胞分裂素处理后前质体向造粉体的转化。因此，可以说，即使在短短的两年时间内，对烟草BY-2的细胞和分子生物学的理解也取得了重大进展，从而总体上理解了植物细胞的基本现象。在日本植物学会年会上举行了一个公共论坛，以使更多的人熟悉这个小组的讨论主题。这个国家的圈子。观众的反应非常重要，因为其中一些人希望在他们的研究中引入这个实验系统。少

项目成果

Yasuhara, H., Sonobe, S.and Shibaoka, H.: "Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells." Eur. J.Cell Biol. 66. 274-281 (1995)

Yasuhara, H.、Sonobe, S. 和 Shibaoka, H.：“布雷菲德菌素 A 对烟草 BY-2 细胞中细胞板形成的影响。”

Shibaoka, H., Asada, T., Yamamoto, S.and Sonobe, S.: "The use of model systems prepared from tobacco BY-2 cells for the studies of the cytoskeleton." J.Microscopy. 181. 14-12 (1996)

Shibaoka, H.、Asada, T.、Yamamoto, S. 和 Sonobe, S.：“使用烟草 BY-2 细胞制备的模型系统来研究细胞骨架。”

Kumagai,F.et al.: "The involvement of protein synthesis elongation factor 1 α in the organization of microtubules on the perinuclear region during the cell cycle transition from M phase to G_1 phase in tobacco BY-2 cells." Botanica Acta. 108. 467-473 (199

Kumagai, F. 等人：“烟草 Botanica Acta 108 细胞从 M 期向 G_1 期转变期间，蛋白质合成延伸因子 1 α 参与核周区域的微管组织。” 467-473 (199

Matsuoka, K., Bassham, D.C., Raikhel, N.V.and Nakamura, K.: "Different sensitivity ot wortmannin of two vacuolar sorting signals indicates the process of distinct sorting machineries in tobacco BY-2 cells." J.Cell Biol.130. 1307-1318 (1995)

Matsuoka, K.、Bassham, D.C.、Raikhel, N.V. 和 Nakamura, K.：“两个液泡分选信号对渥曼青霉素的不同敏感性表明烟草 BY-2 细胞中不同分选机制的过程。”

Kusaba,M.et al.: "A multiple-stimuli-responsive as-1-related element of parA gene confers responsiveness to cadmium but not copper." Plant Physiol.111. 1161-1167 (1996)

Kusaba,M.等人：“parA 基因的多重刺激响应 as-1 相关元件赋予对镉的响应性，但对铜没有响应性。”