Molecular and Cellular Biology of Tobacco BY-2 Cell Line

烟草 BY-2 细胞系的分子和细胞生物学

基本信息

批准号：
07304053
负责人：
NAGATA Toshiyuki
金额：
$ 7.3万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

This project has been intended and conducted to facilitate the acceleration of research progress and information change on the study of molecular and cellular biology of tobacco BY-2 cell line. This subject is quite unique, since this is only one available highly synchronized system in the world that has been developed by the principal investigator and has been distributed to more than 100 laboratories in at least 20 countries. This is indispensable in particular for the cell cycle studies and is being used in major laboratories in the world. Thus' it was intended in this study to facilitate the informational exchange and romote the progress of scientific studies using this cell line in this Country as well.As aconsequence after two years' efforts, it was shown by using highly synchronized cell populations that molecular mechanism of the organization of cortical microtubules has been clearly demonstrated and several types of kinesin molecules oscillated differently during cell cycle pr … More ogression. It is also revealed that a novel auxin-signal transduction chain is involved in the maintanance of cell division activity in tobacco BY-2cells. Furthermore, the development of a novel in vitro transcription system by one of collaborators using BY-2 cells facilitated further the popularity of this cell line at the international level. It was also shown that there are two different transport systems of proteins into vacuoles that are dependent on unique sequence in the C-terminal and N-terminal sequences of proteins, respectively. Conversion of proplastids to amyloplasts upon the treatment by cytokinin has also been analyzed by one investigator. Thus, it may be said that even within the short frame of two years significant progress of the understanding of the cellular and molecular biology on tobacco BY-2 has been attained, resulting in understanding the basic phenomenon of plant cells in general.In 1995, a public forum was held at the occasion of Annual Meeting of the Botanical Society of Japan to familiarize the topics of discussion in this group to much wider circles in this country. Responses from the audiences were quite significant, since some of these peoples would like to introduce this experimental system in their studies. Less

该项目的目的是为了促进研究进度的加速和信息变化，以研究烟草By-2细胞系的分子和细胞生物学研究。该主题非常独特，因为这只是世界上主要研究人员开发的一个可用的高度同步系统，并已分发给至少20个国家 /地区的100多个实验室。对于细胞周期研究，这是必不可少的，并且正在世界上主要的实验室中使用。 “这是在这项研究中旨在促进信息交流和romote在该国使用该细胞系的科学研究的进步。根据两年的努力，通过使用高度同步的细胞群来表明，皮质微蛋白蛋白蛋白蛋白细胞的分子机制已被明确证明，并且在细胞蛋白分子的几种类型的细胞中表现出了差异。还表明，一种新型的生长素信号转移链参与烟草by-2细胞中细胞分裂活性的维持。此外，使用BY-2细胞的合作者之一，通过合作者进行了一种新型的体外转录系统的发展，进一步使该细胞系在国际层面上的普及。还表明，蛋白质的蛋白质传输系统分别取决于蛋白质的C末端和N末端序列中的独特序列。一位研究者还分析了细胞分裂素治疗后，预防性对淀粉样细胞的转化。可以说，即使在两年的短期内，也已经达到了对烟草和分子生物学的理解的重大进展，也达到了理解，从而理解了一般的植物细胞的基本现象。在1995年，在1995年举行了一个公共论坛，在日本植物学会年度会议之际举行了一个在这个小组中熟悉这个国家的讨论的主题，以在这个国家中熟悉这个国家。受众的反应非常重要，因为其中一些人希望在他们的研究中引入这种实验系统。较少的