Elucidation of the new signal transition pathway regarding auxin

阿尔索夫·奥辛被发现为第一个植物荷尔蒙，但其分子机制至少是不可理解的。所以，这个研究是-旨在从auxin应用程序中提取信号转换路径。根据我们先前的研究，我们已经确定了auxin调节的基因，第A节、第B节和第C节，我们进一步向这些基因展示了特征化的auxin反应性元素，并在随后的章节中展示了它们与这些元素的特定绑定蛋白质，因此我们可以在auxin信号的路径中参与。在这个意义上，我们找到了细胞因子，是谁要求在烟草中间蛋白质原型中进行细胞分裂的定义，我们找到了一个关键的角色来扮演进入细胞循环。这似乎表明细胞周期条目是由细胞素触发的，细胞周期进程是由第A、第B和第C类经规范的精氨酸基因产品的，以及谷氨酰胺S-转移的编码不同类型的基因。这是一个完整的新解释。 ... More 在活性细胞分区中输入烟草中间蛋白质原塑料进入细胞循环加速，结果是在另一方，描述烟草BY-2细胞繁殖的auxin信号路径，我们已识别出一个auxin regualted基因， arcA，在auxin starved BY-2细胞中添加了auxin信号的路径。在这种情况下，KI-D1 + I-D1离子通道蛋白质的β-亚单位被提议参与，但我们没有对这条道路进行决定性的整合。However，我们已经确定了另一条完全新的奥辛信号路径。当我们将生长素生长的2B-13个细胞过滤到生长素生长的BY-2细胞时，我们可以看到这些细胞中细胞分化的定义。这对低分子重量植物在文化过滤中的荷尔蒙很热，但更倾向于高分子质量蛋白质。通过凝胶过滤使用Sephadex G25揭示的蛋白质的进一步提炼，因为它是正确的。30 kDa蛋白质。虽然这个蛋白质的本质还没有被确定，但这确实是一个新的信号路径，从奥斯汀应用程序下游，也有报告说没有这样的路径。Less（低）

Although auxin was discovered as the first plant hormone, its molecular mechanism is least understood. Thus, this study was-attempted to elucidate signal transduction pathways downstream from auxin application. As we have identified auxin-regulated genes, parA, parB and parC, in our previous study, we further characterized auxin-responsive elements to these genes and subsequetnly showed that there are specific binding proteins to these elements, which are supposed to be involved in auxin-signalling pathways. In the meantime, we found that cytokinin, which is requested for the induction of cell division in tobacco mesophyll protoplasts, was found to play a critical role for the entry into cell cycle. It seems that cell cycle entry was triggered by cytokinin and the cell cycle progression was further facilitated by the gene products of auxin-regulated genes of parA, parB and parC, which encode different types of glutathione S-transferases. This is a completely new interpretation for the … More entry of tobacco mesophyll protoplasts into cell cycle progression, resulting in active cell division.On the other hand, regarding auxin-signalling pathways for tobacco BY-2 cell proliferation, we have identified an auxin-regualted gene, arcA, upon the addition of auxin to the auxin-starved BY-2 cells. In this case, involvement of β-subunit of KィイD1+ィエD1-ion channel proteins was proposed, but we have not gotten decisive conclusion for this pathway. However, we have identified another completely new pathway for auxin signalling. When we added culture filtrates of auxin-habituated 2B-13 cells to the auxin-starved BY-2 cells, we could see the induction of cell division in these cells. This was hot due to the low molecular weight plant hormones in the culture filtrates, but rather to higher molecular mass proteins. Further elucidation of the protein by a gel filtration using Sephadex G25 revealed that it was due to ca. 30 kDa protein. Though the nature of this protein has not been determined yet, it is certain that there is a new signalling pathway downstream from auxin application, as there has been reported no such pathways. Less