Regulation of cell function by ion channels.

通过离子通道调节细胞功能。

• 批准号: 07307001
• 负责人: NOMA Akinori
• 金额: $ 5.44万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07307001/
• 关键词: Ion channel; Cell differentistion; transporter; receptor; cardiac muscle; smooh muscle; neuron

Akaike acutely dissociated central neurons and performed the whole cell voltage clamp. It was found that the response to N-methyl-D-aspartate was decreased with age of the experimental animal, and this decrease was attributed to a decrease in the distribution density of the receptor. Inoue found that the K^+ ions are actively removed from the space between the nerve axonal membrane and the Schwann cells. Ohmori examined the synaptic transmission within MNTB in the newborn rat and showed that the transmission is established at postnatal day 4 and the increase in the Ca^<2+> current underlies the development of the synaptic transmission. Okada examined the role of the volume-sensitive Cl-channel in the cell-volume regulation, and discussed the relationship between the secretion of the epithelial cells and the Cl-channel. Kameyama examined the intracellular factor, which maintains the activity of the Ca^<2+> channel, and indicated that this factor is dependent on some unknown protein and … More ATP within the cell. Kitasato examined the spontaneous rhythm in the membrane potential change in the B cell in the pancreas, and clarified that changes in intracellular concentration of ATP,and the release of glucagon from A cells are involved. Kuba recorded the changes in the cytoplasmic Ca^<2+> concentration in the nerve terminal and suggested that the Ca^<2+>-induced Ca^<2+> release is responsible for the transmitter release. Kurachi succeeded in cloning a new SUR subtype and indicated that this new subtype is responsible for the ATP sensitive K channel in the smooth muscle. Suzuki investigated the stretch-activated channel in the gastic smooth muscle. Seyama proposed a hypothesis that the intracellular Mg inactivates the L-type Ca^<2+> channel and provided several experimental evidence to support the hypothesis. Takahashi investigated the role of bFGF in the neural induction in the Tunicate embryo, and proposed that bFGF is an ubiquitous factor in the neural induction. Tonosaki prepared a monoclonal antibody for the retinal cells and investigated the role of the target proteins in the sensory signal transduction. Horie investigated the effect of angiotensin II on the cardiac myocytes. Yamagishi examined the properties of the Cl-channels in the ganglion cells. Noma measured the cell volume of single cardiac myocytes and indicated that the cAMP-regulated Cl-channels are involved in the cell volume regulation. Less

Akaike急性分离中枢神经元并进行全细胞电压钳。发现对N-甲基-D-天冬氨酸的反应随着实验动物的年龄而降低，并且这种降低归因于受体分布密度的降低。Inoue发现，K^+离子被主动地从神经轴突膜和雪旺细胞之间的空间中移除。Ohmori检查了新生大鼠MNTB内的突触传递，并表明该传递在出生后第4天建立，并且Ca^2+电流的增加是突触传递发展的基础。Okada研究了体积敏感性Cl-通道在细胞体积调节中的作用，并讨论了上皮细胞分泌与Cl-通道之间的关系。龟山研究了维持Ca^2+通道活性的细胞内因子，并指出该因子依赖于某种未知的蛋白质， ...更多信息 细胞内的ATP。北里研究了胰腺B细胞膜电位变化的自发节律，并阐明了细胞内ATP浓度的变化和A细胞胰高血糖素的释放。库巴记录了神经末梢细胞质Ca^<2+>浓度的变化，并表明Ca^<2+>诱导的Ca ^<2+>释放是递质释放的原因。Kurachi成功地克隆了一个新的SUR亚型，并指出这个新的亚型负责平滑肌中的ATP敏感性K通道。Suzuki研究了胃平滑肌中的牵张激活通道。Seyama提出了细胞内Mg使L型Ca^<2+>通道失活的假说，并提供了几个实验证据来支持这一假说。Takahashi研究了bFGF在被囊动物胚胎神经诱导中的作用，并提出bFGF是神经诱导中普遍存在的因子。Tonosaki制备了视网膜细胞的单克隆抗体，并研究了靶蛋白在感觉信号转导中的作用。Horie研究了血管紧张素II对心肌细胞的影响。Yamagishi检查了神经节细胞中Cl-通道的特性。Noma测量了单个心肌细胞的细胞体积，并表明cAMP调节的Cl-通道参与了细胞体积的调节。少

项目成果

Saitoe M: "Neuronal Expression in Cleavage-Arrested Ascidian Blastomeres Requires Gap Junctional Uncoupling from Neighboring Cells" Journal of Physiology. 491. 825-842 (1996)

Saitoe M：“分裂停滞的海鞘卵裂球中的神经元表达需要与邻近细胞的间隙连接解偶联”生理学杂志。

Xu.T.L.: "Protein kinase C-mediated enhancement of glycine response in rat sacral dorsal commissural neurones by serotonin." Journal of Physiology. 496. 491-501 (1996)

Xu.T.L.：“蛋白质激酶 C 介导的血清素增强大鼠骶背连合神经元的甘氨酸反应。”

Xu, T. L.: "Protein-Kinase C-Mediated Enhancement of Glycine Response in Rat Sacral Dorsal Commissural Neurons by Serotonin" Journal of Physiology. 496. 491-501 (1996)

Xu, T. L.：“蛋白质激酶 C 介导的血清素增强大鼠骶背连合神经元的甘氨酸反应”生理学杂志。

Teramoto N: "Effects of calciseptine on unitary basium channel currents in guiner-pig potal vein" Pfligers Archiv. 432. 462-470 (1996)

Teramoto N：“钙化肽对豚鼠静脉中单一碱通道电流的影响”Pfligers Archive。

Tominaga, M: "Glibenclamick, an ATP-seusiture E channel blocfur, inhibd cAMP-aetuiateel I-conductane" Circulation Research. 77. 417-423 (1995)

Tominaga, M：“Glibenclamick，一种 ATP-seusiture E 通道阻断剂，抑制 cAMP-aetuiateel I-conductane”循环研究。