Exploitation of planar platinum complex systems for luminescence probing of microenvironment in solution with the application to a DNA intercalation probe.

新的发光探针，Pt （II）螯合物的8-喹啉酚衍生物的研究，作为“探针”用于微环境的表面活性剂胶束和DNA凹槽。我们的目标是建立基于DNA与螯合物相互作用的体内微环境DNA测定系统。我们研究了作为微环境模型的配体- 8-喹啉衍生物和表面活性剂胶束。研究了母体化合物8-喹啉酚及其衍生物5-磺基-8-喹啉酚（5sox）、7-碘-5-磺基-8-喹啉酚（铁）和一个三齿化合物2-（2′-羟基苯基）-8-喹啉酚。这些配体的螯合物在表面活性剂胶束介质中表现出强烈的红色发光。特别是，pt -铁配合物在CTAC阳离子表面活性剂胶束和亚硫酸盐离子作为溶解氧的去除剂的存在下，其发光强度提高了约34倍。发光寿命在μ s级（5sox螯合物为31 μ s），远远长于普通金属离子螯合物的ns级。因此，其发光为室温磷光。这些结果对于理解探针的DNA嵌入是未来研究的主题具有重要意义。由于Pt配合物为阴离子或非离子，因此研究了阳离子（季铵型）、非离子（聚氧乙烯型和亲水糖基型）表面活性剂。在任何表面活性剂中，发光发展都需要高于CMC的浓度。一种糖型表面活性剂n-十二烷基-d -麦芽糖甙在所有表面活性剂中显示出最大的发光增强。我们期望详细地确定发光Pt螯合物的光谱性质与螯合物-胶束相互作用之间的关系。

New luminescence probes, Pt (II) chelates of 8-quinolinol derivatives are examined to be used as "probes" for microenvironments given by surfactant micelles and DNA grooves. We aimed to produce the determination system of DNA as microenviroment in vivo based on an interaction between DNA and the chelates.We investigated the ligands 8-quinolinolate derivatives and surfactants micelles which serve as microenvironment models.1. The mother compound 8-quinolinol and the sulfo derivatives 5-sulfo-8-quinolinol (5sox), 7-iodo-5-sulfo-8-quinolinol (ferron), and a tridentate 2-(2'-hydroxyphenyl)-8-quinolinol have been studied. The chelates of these ligands show strong red luminescence in surfactants micelle media. Particularly, Pt-ferron complex are observed to increase luminescence intensity about thirty four times in the presence of CTAC cationic surfactants micelles and sulfite ion as the remover of dissolved oxygen. The life times of the luminescence are in mu s levels (31 mu s for 5sox chelate), which are much longer than those in the ns levels of ordinary metal ion chelates. Therefore, the luminescence is room temperature phosphorescence. These results are an important knowledge to understand DNA intercalation of the probes which is the subject of future research.2. Surfactants, cationic (quaternary ammonium type), nonionic (polyoxyethylene type and hydrophilic sugar group type), have been studied because the Pt complexes are anion or nonionic. In any surfactants, luminescence developments need the concentration of that above the CMC.A sugar type surfactant, n-dodecyl-beta-D-maltoside shows the largest enhancement of the luminescence among all surfactants examined. We expect to determine a relation between the spectroscopic properties of the luminescence Pt chelates and the chelate-micelle interactions in detail.