Exploitation of planar platinum complex systems for luminescence probing of microenvironment in solution with the application to a DNA intercalation probe.

8-喹啉类化合物的铂(II)络合物是一种新型的发光探针，可作为表面活性剂胶束和DNA沟槽微环境的“探针”。我们的目标是基于DNA和络合物之间的相互作用，建立DNA作为微环境的体内测定体系。我们研究了作为微环境模型的配体8-喹啉衍生物和表面活性剂胶束。研究了母体化合物8-喹啉及其磺基衍生物5-磺酸-8-喹啉(5sox)、7-碘-5-磺酸-8-喹啉(Ferron)和三齿化合物2-(2‘-羟基苯基)-8-喹啉。这些配体的络合物在表面活性剂胶束介质中表现出强烈的红色发光。特别是，在CTAC阳离子表面活性剂胶束和亚硫酸盐离子作为溶解氧的去除剂的存在下，铂-铁龙络合物的发光强度提高了约34倍。其发光寿命为亩S水平(5sox络合物为31亩S)，远长于普通金属离子络合物的ns水平。因此，发光是室温磷光。这些结果是理解DNA嵌入探针的重要知识，这是未来研究的主题。由于铂的络合物是阴离子或非离子的，人们研究了阳离子(季铵盐型)、非离子(聚氧乙烯型和亲水性糖基型)表面活性剂。在所有表面活性剂中，发光的形成需要CMC以上的浓度。糖类表面活性剂N-十二烷基-β-D-麦芽糖苷在所考察的表面活性剂中显示出最大的发光增强作用。我们期望详细地确定发光铂络合物的光谱性质与络合物-胶束相互作用之间的关系。

New luminescence probes, Pt (II) chelates of 8-quinolinol derivatives are examined to be used as "probes" for microenvironments given by surfactant micelles and DNA grooves. We aimed to produce the determination system of DNA as microenviroment in vivo based on an interaction between DNA and the chelates.We investigated the ligands 8-quinolinolate derivatives and surfactants micelles which serve as microenvironment models.1. The mother compound 8-quinolinol and the sulfo derivatives 5-sulfo-8-quinolinol (5sox), 7-iodo-5-sulfo-8-quinolinol (ferron), and a tridentate 2-(2'-hydroxyphenyl)-8-quinolinol have been studied. The chelates of these ligands show strong red luminescence in surfactants micelle media. Particularly, Pt-ferron complex are observed to increase luminescence intensity about thirty four times in the presence of CTAC cationic surfactants micelles and sulfite ion as the remover of dissolved oxygen. The life times of the luminescence are in mu s levels (31 mu s for 5sox chelate), which are much longer than those in the ns levels of ordinary metal ion chelates. Therefore, the luminescence is room temperature phosphorescence. These results are an important knowledge to understand DNA intercalation of the probes which is the subject of future research.2. Surfactants, cationic (quaternary ammonium type), nonionic (polyoxyethylene type and hydrophilic sugar group type), have been studied because the Pt complexes are anion or nonionic. In any surfactants, luminescence developments need the concentration of that above the CMC.A sugar type surfactant, n-dodecyl-beta-D-maltoside shows the largest enhancement of the luminescence among all surfactants examined. We expect to determine a relation between the spectroscopic properties of the luminescence Pt chelates and the chelate-micelle interactions in detail.