Exploitation of planar platinum complex systems for luminescence probing of microenvironment in solution with the application to a DNA intercalation probe.

利用平面铂络合物系统发光探测溶液中的微环境并应用于 DNA 嵌入探针。

• 批准号: 07455438
• 负责人: HOSHINO Hitoshi
• 金额: $ 0.9万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07455438/
• 关键词: Platinum complex; Room temperature phosphorescence; Luminescent probe; Microenviroment; Long lived luminescence; 長寿命発光; 白金; ミセル; 8-キノリノール; インターカレーション

New luminescence probes, Pt (II) chelates of 8-quinolinol derivatives are examined to be used as "probes" for microenvironments given by surfactant micelles and DNA grooves. We aimed to produce the determination system of DNA as microenviroment in vivo based on an interaction between DNA and the chelates.We investigated the ligands 8-quinolinolate derivatives and surfactants micelles which serve as microenvironment models.1. The mother compound 8-quinolinol and the sulfo derivatives 5-sulfo-8-quinolinol (5sox), 7-iodo-5-sulfo-8-quinolinol (ferron), and a tridentate 2-(2'-hydroxyphenyl)-8-quinolinol have been studied. The chelates of these ligands show strong red luminescence in surfactants micelle media. Particularly, Pt-ferron complex are observed to increase luminescence intensity about thirty four times in the presence of CTAC cationic surfactants micelles and sulfite ion as the remover of dissolved oxygen. The life times of the luminescence are in mu s levels (31 mu s for 5sox chelate), which are much longer than those in the ns levels of ordinary metal ion chelates. Therefore, the luminescence is room temperature phosphorescence. These results are an important knowledge to understand DNA intercalation of the probes which is the subject of future research.2. Surfactants, cationic (quaternary ammonium type), nonionic (polyoxyethylene type and hydrophilic sugar group type), have been studied because the Pt complexes are anion or nonionic. In any surfactants, luminescence developments need the concentration of that above the CMC.A sugar type surfactant, n-dodecyl-beta-D-maltoside shows the largest enhancement of the luminescence among all surfactants examined. We expect to determine a relation between the spectroscopic properties of the luminescence Pt chelates and the chelate-micelle interactions in detail.

新的发光探针，铂（II）螯合物的8-羟基喹啉衍生物被检查作为“探针”的表面活性剂胶束和DNA凹槽的微环境。本研究以DNA与螯合物的相互作用为基础，建立了DNA作为体内微环境的检测体系，研究了8-羟基喹啉衍生物配体和表面活性剂胶束作为体内微环境模型.研究了母体化合物8-羟基喹啉及其磺基衍生物5-磺基-8-羟基喹啉（5sox）、7-碘-5-磺基-8-羟基喹啉（ferron）和三齿2-（2 '-羟基苯基）-8-羟基喹啉。这些配体的螯合物在表面活性剂胶束介质中显示出强烈的红色发光。特别是在CTAC阳离子表面活性剂胶束和亚硫酸根离子作为溶解氧去除剂的存在下，观察到Pt-ferron络合物的发光强度增加约34倍。发光寿命为μ s级（5sox螯合物为31 μ s），比普通金属离子螯合物的ns级发光寿命长得多。因此，发光是室温磷光。这些结果对于理解探针的DNA嵌入是一个重要的知识，是未来研究的主题.表面活性剂，阳离子（季铵型），非离子（聚氧乙烯型和亲水性糖基型），已被研究，因为铂配合物是阴离子或非离子。在所有的表面活性剂中，发光的发展需要CMC以上的浓度，糖型表面活性剂正十二烷基-β-D-麦芽糖苷在所有的表面活性剂中显示出最大的发光增强。我们期望详细地确定发光Pt螯合物的光谱性质与螯合物-胶束相互作用之间的关系。