Development of Capillary Electriphoretic Reactor and Its Application to Kinetically High-Peformance Metal Complexes

毛细管电泳反应器的研制及其在动力学高性能金属配合物中的应用

• 批准号: 13650864
• 负责人: HOSHINO Hitoshi
• 金额: $ 2.62万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13650864/
• 关键词: Metal Complexes; Capillary Electrdophoresis; Dissociation Rate Constants; Capillary Electrophoretic Reactor; Kinetically High-Peformance Metal Complexes; Metal Ion Analysis; Pre-Column Derivatization Reagents; 解難反応速度; ホルマザン; 高性能液体クロマトグラフィー; ベリリウ

The capillary electrophoretic reactor (CER) has been developed. The basic idea of CER is to consider the separation process of CE as a chemical reactor for metal complexes. CER method was applied to investigate the dissociation kinetics of lanthanide (III) polyaminocarboxylate complexes and tetradentate cyanoformazane complexes with Zn(II) and Mn(II). In general, complexes of metal ions, such as lanthanide ions, Zn(II) and Mn(II), had been thought to be labile because of large substitution rate constants of coordinated water molecules at the metal ions. On the contrary, it was found that these camplexes had been inert to be amenable to the detection in pre-capillary derivatization CE system with no addition of the ligand in a carrier buffer solution.Inquires into new kinetically high-peformance metal complexes has been made. Thiacalix[4]arenetetrasulfonate (TCAS) and 2-(2'-Hydroxyphenyl)-10-hydroxybenzo[h]quinoline (HPHBQ) were examined as a pre-column chelating reagent for the determination of ultra trace Ni(II) and Be(II) by KD-HPLC of the pre-column derivatization HPLC system with no addition of the ligand in an eluent, respectively. The practical applicability of KD-HPLC methods with TCAS and HPHBQ was also demonstrated.

研制了毛细管电泳反应器（CER）。CER的基本思想是将CE的分离过程视为金属络合物的化学反应器。采用CER方法研究了镧系多氨基羧酸配合物和四齿氰基甲腈配合物与Zn（II）和Mn（II）的离解动力学。通常，金属离子的络合物，如镧系元素离子、Zn（II）和Mn（II），被认为是不稳定的，因为配位水分子在金属离子处的大的取代速率常数。相反，这些配合物在毛细管前衍生毛细管电泳体系中不加配体，具有惰性，可用于检测，并对新的动力学高效金属配合物进行了探讨。本文研究了硫杂杯[4]芳烃四磺酸盐（TCAS）和2-（2 '-羟基苯基）-10-羟基苯并[h]喹啉（HPHBQ）作为柱前螯合剂，在不加配体的洗脱液中，柱前衍生-KD-HPLC法测定超痕量Ni（II）和Be（II）。还证明了KD-HPLC法与TCAS和HPHBQ的实用性。

项目成果

H. Matsumiya, T. Ishida, N. Iki, and S. Miyano: "Thiacalix[4]arenetetrasulfonate as Specific Pre-column Chelating Reagent for Nickel(II) Ion in Kinetic Differentiation Mode High-performance Liquid Chromatography"Analytica Chimica Acta. 478(2). 163-170 (20

H. Matsumiya、T. Ishida、N. Iki 和 S. Miyano：“Thiacalix[4] 芳烃四磺酸盐作为动态分化模式高效液相色谱中镍 (II) 离子的特异性柱前螯合剂”分析化学学报。

H.Matsumiya, T.Ishida, N.Iki, S.Miyano: "Thiacalix[4]arenetetrasulfonate as Specific Pre-column Chelating Reagent for Nickel(II) Ion in Kinetic Differentiation Mode High-performance Liquid Chromatograhy"Analytica Chimica Acta. 478(2). 163-170 (2003)

H.Matsumiya、T.Ishida、N.Iki、S.Miyano：“Thiacalix[4]芳烃四磺酸盐作为动态分化模式高效液相色谱中镍 (II) 离子的特异性柱前螯合剂”Analytica Chimica Acta。

Hiroaki Matumiya, Hitoshi Hoshino: "Selective Determination of Beryllium(II) Ion at Picomole per Decimeter Cubed Levels by Kinetic Differentiation Mode Reversed-Phase High-Purformance Liquid Chromatography with Fiuorometric Detection Using 2-(2'-Hydroxyph

Hiroaki Matumiya、Hitoshi Hoshino：“通过动力学微分模式反相高效液相色谱法以及使用 2-(2-Hydroxyph 荧光检测) 选择性测定每分米立方皮摩尔水平的铍 (II) 离子

Hiroaki Matsumiya, Hitoshi Hoshino: "Selective Determination of Beryllium(II) Ion at Picomole per Decimeter Cubed Levels by Kinetic Differentiation Mode Reversed-Phase High-Performance Liquid Chromatography with Fluorometric Detection Using 2-(2'-Hydroxyp

Hiroaki Matsumiya、Hitoshi Hoshino：“通过动力学微分模式反相高效液相色谱法和荧光检测，使用 2-(2-Hydroxyp) 选择性测定每分米立方皮摩尔水平的铍 (II) 离子

壹岐 信彦: "超微量金属錯体の速度論的識別モード-キャピラリー電気泳動システムの設計"分析化学. 51(7). 495-506 (2002)

Nobuhiko Iki：“超痕量金属配合物的动力学辨别模式 - 毛细管电泳系统的设计”分析化学 51（7）（2002）。