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Physiological functions of crops transformed with Agrobacterium spp. and its agricultural use

农杆菌转化作物的生理功能。

基本信息

批准号：
07456011
负责人：
YOSHIDA Ryuji
金额：
$ 2.69万
依托单位：
Toyama Prefectural University, College of Technology (TPU)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456011/
关键词：
Agrobacterium spp.TPU strains Opine types rol genes High cotents of ZR & ABA Agrobacterium tumefacience Seed-propagation sweet potato Transgenic sweet potato アグロバクテリウムミキモピン型カルス・不定根・不定芽不定芽の培養形質転換体作物の作出生理機能の解析 TPU菌株の細菌学的性質

项目摘要

1. Sixty Japanese wild-strains of Agrobacterium spp. were isolated from crown galls of Rosa multiflora thunb. and cultivars of Rosa in Toyama districts. they were numbered and accepted as the strains of TPU (Toyama Prefectural University).2. Among their TPU strains, TPU-50, -54, -55 and-57 strains were identified as Agrobacterium tumefacience biovar 2 from the date of bacteriological natures, and other strains were different from Agrobacterium spp.3. The results of southern hybridization analysis showed that the TPU-strains isolation here could be classified into five unique T-DNA types distinct from authentic Agrobacterium strains, NIAES 1724 and A13 types. Opine type in tissues transformed with TPU- strains was nopaline, agropine and mikimopine types. Almost all of TPU-strains had rol B and rol C genes.4. As for the contents of cytokinins and abscisic acid in transformed tissues, the crown gall and hypocotyle explant tissues had relatively high amount of zeatin riboside and cis-abscisic acid.5. When the bacterial cells of TPU-57 or TPU-58 strains were inoculated on the top of the hypocotyle sections obtained from seed-propagation Sweet potato, tumor or callus formation were observed on the inocutated surface. After about 50 days of subcul turing, the transformed plants were regenerated from these callus on MS medium containing vancomycin and carbenicillin.On the results obtaineed here, we wish to study in future adout the alterations of endogenous cytokinins and ABA(s) in crop plants transformed by TPU-strains.

1.60株日本野生农杆菌。从多花月季的树冠瘤中分离到2株。富山地区的月季和品种。它们被编号并被接受为TPU(富山县立大学)的菌株。其中TPU-50、-54、-55和-57菌株经细菌学鉴定为根癌农杆菌生物型2，其余菌株与农杆菌属不同。Southern杂交分析结果表明，分离的TPU菌株可分为5个不同于农杆菌正品菌株的独特的T-DNA型，即NIAES 1724和A13。TPU-菌株转化的组织中的鸦片素型为诺帕林型、阿罗平型和米基莫平型。几乎所有的TPU-菌株都有rol B和rol C基因。从转化组织中细胞分裂素和脱落酸的含量来看，冠瘤和下胚轴外植体组织中玉米素核苷和顺式脱落酸的含量相对较高。将TPU-57或TPU-58菌株的细菌细胞接种于甘薯种子繁殖的下胚轴切片上，接种表面可见肿瘤或愈伤组织形成。继代培养约50天后，这些愈伤组织在含有万古霉素和羧苯霉素的MS培养基上再生植株。在此基础上，我们希望进一步研究TPU-菌株转化作物植株中内源细胞分裂素和脱落酸(S)的变化。