课题基金 / 基金详情

Which is the origin of the Phytoplasma, Plants or Insects?

Which is the origin of the Phytoplasma, Plants or Insects?
植原体、植物或昆虫的起源是什么?
批准号:
07456026
负责人:
NUMBA Shigetou
金额:
$5.06万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1996

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项目成果

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中文摘要
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英文摘要
We have analyzed phytplasma in terms of molecular evolutionism, and studied in order to establish systems of genome analysis and molecular diagnosis. First, we gathered various separated roots of phytoplasma and refined the genome by the CTAB method. Then we amplified 16SrRNA genes by PCR and determined the whole base arrangemant by direct sequences. Further, we refered the newest base data of 16SrRNA genes in microbes including phytoplasma in other countries, using data base of the large computer, and compared the results to the base arrangement data we had before. Therefore, We have constructed the molecular genealogical family tree by large analysis program "ODEN" and proceeded "Bootstrap" to analyze reliablility. Under the analyzed data, we have established the classifying system of phytoplasma. Further, we have applyed the classification of "species" to phytoplasma by the above data and system, designed and composed the unique primer. Also we have tried to classify the unknown separated roots of phytoplasma in the various area of Asia.
期刊论文(12)
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科研奖励(0)
会议论文
Sato,M.Nanba.S.,Katsuharu,M.,Kanakita,H.,Mitsuhashi,W.,Kannbe,S.: "PCR(Polymcrase Chain Reaction)Detecbion of Phytoplasma in Phloon Sap Collected by Laser Sbylectomy" 日本植物病理学会報. 62・2. 177-180 (1996)
Sato, M. Nanb​​a.、Katsuharu, M.、Kanakita, H.、Mitsuhashi, W.、Kannbe, S.:“PCR(聚合酶链式反应)检测激光 Sbyl 切除术收集的 Phloon 汁液中的植原体”日本植物病理学学术通报62・2。
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通讯作者:
Sato,M.,Namba,S.,Katsuhara,M,Kawakita,H.,Mitsuhashi,W & Kawabe,S:"PCR(Polymerase Chain Reaction)Detection of Phytoplasema in Phloem Sap Collected by Laser Stylectomy" 日本植物病理学会報. 62・2. 177-180 (1996)
Sato, M.、Namba, S.、Katsuhara, M、Kawakita, H.、Mitsuhashi, W 和 Kawabe, S:“激光茎切除术收集的韧皮部汁液中植原体的 PCR(聚合酶链式反应)检测” 日本学会杂志植物病理学 62・2。
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通讯作者:
Ohira,K.,Namba,S.,Rozanov,M.,Kusumi,T.and Tsuchizaki,T.: "Complete sequence of an infectious full-length cDNA clone of citrus tatter leaf capillovirus : comparative sequence analysis of capillovirus genomes." J. General Virol.76. 2305-2309 (1995)
Ohira,K.、Namba,S.、Rozanov,M.、Kusumi,T. 和 Tsuchizaki,T.:“柑橘碎叶毛状病毒感染性全长 cDNA 克隆的完整序列:毛状病毒基因组的比较序列分析。”
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通讯作者:
11
    Establishement of genetic resource of plants against multispectrum disease by using peptide sequences
    • 批准号:
      07556071
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $9.22万
    • 财政年份:
      1995
    • 负责人:
      NUMBA Shigetou
    • 依托单位: