Establishement of genetic resource of plants against multispectrum disease by using peptide sequences

在本项目中，我们开展了以下工作：我们建立了增加几种预防功能的技术，如去外壳，复制，在细胞或身体之间移动，以及病毒蛋白的成熟。此外，我们还试图培育具有病毒属以外最新抗性的植物，建立抗多谱病害的植物遗传资源。首先，我们培育了具有马铃薯Y病毒、红豆花叶病毒、烟草蚀纹病毒、柑橘碎叶病毒和马铃薯T病毒的运动蛋白和外壳蛋白的植物，并分析了每种蛋白的功能。在此基础上，我们获得了柑橘碎叶病毒基因组全长cDNA克隆，构建了侵染性转录RNA，建立了柑橘碎叶病毒的接种侵染体系。在此基础上，我们对cDNA进行了突变，研究了突变对蛋白功能的影响，获得了大量的信息。因此，将多种蛋白质应用于植物，培育具有多谱抗性的植物，将具有重要的意义。

In this project, we have developed our work as follows :We have established the technique to add several preventing functions such as de-integument, copying, moving between cells or a body, and maturity of virus protein. Further, we have tried to produce a plant which has the newest resistant properties beyond genus of virus, and establish genetic resource of plants against multispectrum disease.First, we have produced plants which have potato virus Y,azuki bean mosaic virus, tobacco etch virus, citrus tatter leaf virus, and movement protein and coat protein of potato virus T.Then we have analyzed the functions of each protein. Proceedingly, we have produced full length of cDNA clones of citrus tatter leaf virus genome, composed infectious transcribing RNA and established a system to infect plants by inoculation. Under these results, we have introduced a mutation to the cDNA,investigated influences to the function of the protein, and got a lot of informations. Therefore, it would be possible to get significant knowledges to apply various protein to plants and produce plants which have multispectrum resistant properties in the future.