Selectivity for Incorporation of n-3 and n-6 Unsaturated Fatty Acids into Phospholipids

将 n-3 和 n-6 不饱和脂肪酸选择性掺入磷脂中

• 批准号: 07456062
• 负责人: KITO Makoto
• 金额: $ 4.35万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456062/
• 关键词: n-3 Unsaturated fatty acids; n-6 Unsaturated fatty acids; Phospholipids

The selective incorporation of n-3 and n-6 unsaturated fatty acids into cell phospholipids was investigated by changing the ratio of n-3 unsaturated fatty acids against n-6 unsaturated fatty acids in the medium where Chinese hamster V79-R cells were grown. Unsaturated fatty acids with lower degrees of unsaturation were abundantly incorporated into phosphatidylcholine independently of n-3 and n-6. Unsaturated fatty acids with highter degrees of unsaturation were more predominantly incorporated into phosphatidylethanolamine. When the difference in the number of double bond between unsaturated fatty acids was more than two. the unsaturated fatty acids with a higher degree of unsaturation was more selectively incorporated into phosphatidylethanolamine than the unsaturated fatty acids with a lower dgree of unsaturation. By the analysis of molecular species. n-3 and n-6 unsaturated fatty acids with higher degrees of unsaturation were incorporated competitively into phosphatidylethanolamine. Finally, docosahexaenoic acid was incorporated into diacyl phosphatidylethanolamine more selectively than the other unsaturaed fatty acids.In addition. the similar selectivity was found in phosphatidylcholine and phosphatidylethanolamine of mitochondria, endoplasmic reticulum and plasma membrane, independent of the difference among organelles.

通过改变中国仓鼠V79-R细胞培养基中n-3和n-6不饱和脂肪酸的比例，研究了n-3和n-6不饱和脂肪酸在细胞磷脂中的选择性掺入。不饱和程度较低的不饱和脂肪酸被大量地结合到磷脂酰胆碱中，独立于n-3和n-6。不饱和度越高的不饱和脂肪酸更多地结合到磷脂酰乙醇胺中。当不饱和脂肪酸之间的双键数目相差超过两个时。不饱和度较高的不饱和脂肪酸比不饱和度较低的不饱和脂肪酸更有选择性地结合到磷脂酰乙醇胺中。通过对分子物种的分析。将不饱和度较高的n-3和n-6不饱和脂肪酸竞争性地掺入磷脂酰乙醇胺。最后，二十二碳六烯酸比其他不饱和脂肪酸更有选择性地结合到二酰基磷脂酰乙醇胺中。线粒体、内质网和质膜的磷脂酰胆碱和磷脂酰乙醇胺具有相似的选择性，与细胞器之间的差异无关。

项目成果

H.Masui, R.Urade, and M.Kito: "Selective Incorporation of n-3 and n-6 Unsaturated Fatty Acids into Aminal Cell Phospholipids" Biosci.Biotech.Biochem.59(4). 644-647 (1995)

H.Masui、R.Urade 和 M.Kito：“将 n-3 和 n-6 不饱和脂肪酸选择性掺入细胞磷脂中”Biosci.Biotech.Biochem.59(4)。

H. Masui, R. Urade, and M. Kito: "Selective Incorporation of n-3 and n-6 Unsaturated Fatty Acids into Aminal Cell Phospholipids" Biosci. Biotech. Biochem.59. 644-647 (1995)

H. Masui、R. Urade 和 M. Kito：“将 n-3 和 n-6 不饱和脂肪酸选择性掺入缩醛细胞磷脂中”Biosci。

H. Masui, R. Urade. and M. Kito: "Selective Incorporation of Polynusaturated Fatty Acids into Organelle Phospholipids of Aminal Cells" Biosci. Biotech. Biochem.61 (印刷中). (1997)

H. Masui、R. Urade 和 M. Kito：“多聚脂肪酸选择性掺入细胞器磷脂”Biosci.61（出版中）。

H.Masui,R.Urade.& M.Kito: "Selective Incorporation of Polyunsaturated Fatty Acids into Organelle Phospholipids of Animal Cell" Biosci.Biotech.Biochem.(印刷中). (1997)

H.Masui、R.Urade. 和 M.Kito：“将多不饱和脂肪酸选择性掺入动物细胞的细胞器磷脂”Biosci.Biotech.Biochem（出版中）。

H.Masui, R.Urade, and M.Kito: "Selective Incorporation of Polynusaturated Fatty Acids into Organelle Phospholipids of Aminal Cells" Biosci.Biotech.Biochem.61 (in press). (1997)

H.Masui、R.Urade 和 M.Kito：“将多聚饱和脂肪酸选择性掺入动物细胞的细胞器磷脂中”Biosci.Biotech.Biochem.61（印刷中）。