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Studies of quality control of proteins in the endoplasmic reticulum.

内质网蛋白质质量控制的研究。

基本信息

批准号：
07308068
负责人：
KITO Makoto
金额：
$ 2.43万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07308068/
关键词：
endoplasmic reticulum quality control protein degradation chaperone システインプロテアーゼ

项目摘要

In order to elucidate molecular mechanisms of the quality control of proteins in the endoplasmic reticulum (ER), a research team was organized. Major research results were :the relationship between hydrophobicity of signal peptides and efficiency of translocation of ER membrane was found (Tagaya), function of PDI as a chaperone was determined and PDI related proteins were cloned in yeast (Kikuchi), involvement of calnexin, Bip and proteasome was found in retention and degradation of mutant secretory proteins (Ikehara), recombinant ER-60 protease was expressed and its active residue and ER retention signal were detemined (Kito, Urade), intracellular localization of microsomal triglyceride transfer protein was determined and its interaction with PDI was observed (Sato), cis element was identified for transcriptional control of Bip by Ssalp (Kohno), supression of expression and transportation of procollagen was shown by HSP47 antisense RNA (Hosokawa), involvement of NLS peptide and casein kinase 2 in phosphorylation of GRP94 was found (Miyata), singal for ER retention of Sec12p was determined and funtion of Rerlp was analyzed (Nakano), increased lysophosphatidylethanolamine upon membrane fusion in the regulated exocytosis was identified (Fukuoka).A reseach meeting was organized and the research results were published (Kito).

为了阐明内质网（ER）蛋白质质量控制的分子机制，组建了一个研究小组。主要研究成果有：发现信号肽疏水性与内质网膜转位效率之间的关系（Tagaya），确定PDI作为分子伴侣的功能并在酵母中克隆PDI相关蛋白（Kikuchi），发现钙联蛋白、Bip和蛋白酶体参与突变分泌蛋白的保留和降解（Ikehara），重组表达 ER-60 蛋白酶并测定其活性残基和 ER 保留信号（Kito，Urade），测定微粒体甘油三酯转移蛋白的细胞内定位并观察其与 PDI 的相互作用（Sato），通过 Ssalp（Kohno）鉴定用于 Bip 转录控制的顺式元件，通过 HSP47 反义显示抑制前胶原的表达和运输 RNA (Hosokawa)，发现 NLS 肽和酪蛋白激酶 2 参与 GRP94 的磷酸化 (Miyata)，确定了 Sec12p ER 保留的信号并分析了 Rerlp 的功能 (Nakano)，确定了受调节的胞吐作用中膜融合后溶血磷脂酰乙醇胺的增加（福冈）。组织了研究会议并发表了研究结果（鬼头）。