Dietary Regulation of Development and Regression of Atherosclerosis in Apo E Deficient Mice

Apo E 缺陷小鼠动脉粥样硬化发展和消退的饮食调节

基本信息

批准号：
07456064
负责人：
IMAIZUMI Katsumi
金额：
$ 5.06万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456064/
关键词：
apo E deficient mice ExHC rats soybean protein arginine methionine serum cholesterol artherosclerosis nitric oxide コレステロールイソフラボンリポタンパク質凍結切片ラット

项目摘要

According to westernization of eating style of Japanese, atherosclerotic heart diseases are getting increased, hence it is desirable to find out eating habits to prevent the diseases. The present study was carried out to investigate the effect of dietary proteins, using two animal models : ExHC rats exhibiting hypercholesterolemia in response to exogenous cholesterol and apo E deficient mice which develop atherosclertic lesions similar to humans.ExHC rats, treated with hypervitamin D and then fed and atherogenic diet for 6 month, exhibited less atherosclerotic lesions when fed soybean protein in comparison with casein. These protein effects were subsequently investigated in apo E deficient mice fed diets with or without cholesterol. In both dietary conditions, the mice fed soybean protein had less lesions. Isoflabons which are an abundant components remaining in the soybean protein preparations were not responsible for the beneficial effects. The beneficial effects were reproduced in mice fed diets containing amino acid mixtures simulated to soybean protein. Since methionine and arginine are abundant in casein and soybean protein, respectively, these amino acids were supplemented to casein of soybean protein based diets, respectively. It was found that the addition of arginine to the casein diet effectively suppressed the lesion development, but the methionine to the soybean protein deit had no adverse effects. The arginine supplementation resulted in an elevation of serum metabolites of nitric oxides to the levels similar to rats fed soybean proteins. In any rats, serum cholesterol level was not correlated to the severity of the lesions.These results demonstrate that dietary soybean protein effectively prevents the development of atherosclerotic lesion and suggest that amino acids or the metabolites derived from soybean protein exert ameliorating effects through direct action on arteries.

根据日本饮食风格的西化，动脉粥样硬化的心脏病正在增加，因此希望发现饮食习惯以防止疾病。进行了本研究以研究饮食蛋白的作用，使用两种动物模型：exhc大鼠响应于外源胆固醇和apo不足的小鼠，它们会形成与人类相似的动脉凝蛋白病变，这些病变与人类相似。ExHC大鼠，与超级五脂蛋白d和Ather otigoter的饮食相似，然后再养成6个月的饮食。与酪蛋白进行比较。随后在喂养有或没有胆固醇的饮食中的APO E缺乏小鼠中研究了这些蛋白质作用。在这两种饮食条件下，喂食大豆蛋白的小鼠的病变较少。在大豆蛋白制剂中剩下的丰富成分的异氟代替者对有益作用概不负责。在饲喂含有模拟大豆蛋白的氨基酸混合物的饮食的小鼠中复制了有益作用。由于酪氨酸和精氨酸在酪蛋白和大豆蛋白中分别丰富，因此这些氨基酸分别补充了基于大豆蛋白饮食的酪蛋白。发现在酪蛋白饮食中添加精氨酸可以有效抑制病变的发育，但是大豆蛋白DEIT的蛋氨酸没有不良作用。补充精氨酸导致一氧化氮的血清代谢产物升高到类似于大豆蛋白的大鼠的水平。在任何大鼠中，血清胆固醇水平都与病变的严重程度无关。这些结果表明，饮食中的大豆蛋白有效地阻止了动脉粥样硬化病变的发展，并表明氨基酸或代谢物源自大豆蛋白exert exert exert exert通过直接对植物作用产生影响的大豆蛋白质会影响。