Molecular analysis of enzymes involved in animal phospholipid biosynthesis
Molecular analysis of enzymes involved in animal phospholipid biosynthesis
批准号:
07457044
负责人:
KANOH Hideo
金额:
$4.1万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1997
中文摘要
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英文摘要
We have been working on diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP). We succeeded for the first time in the cDNA cloning of mouse 35-kDa PAP,and subsequently cloned two human PAP isozymes designated PAP-2a and 2b.Our work showed that PAP is unexpectedly involved in the regulation of germ cell migration and cellular differentiation.We also described the cDNA cloning of DGK-delta. We analyzed the translocation of DGK-alpha upon T-lymphocyte activation, and also identified the catalytic domain of this DGK isozyme. We investigated the calcium-binding properties of EF-hand motifs present in common in alpha-, beta- and gamma isoforms of DGK.
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Sakane.F.: "Molecular cloning of a diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal teil similar to those of the EPH tamily of protgaintyrosino kinases." J.Biol.Chem.(印刷中).
Sakane.F.:“二酰基甘油激酶同工酶的分子克隆,其具有与原酪氨酸激酶的 EPH 家族相似的 pleckstrin 同源结构域和 C 端 teil(正在出版)。”
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Kanoh,H.(共同執筆): "Oxford University press.London,U.K.(印刷中)" Diacylglycerol kinase in “Guide book on calcium binding proteins.
Kanoh, H.(合著者):“牛津大学出版社。英国伦敦(正在印刷中)”《钙结合蛋白指南》中的二酰基甘油激酶。
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Yamada, K., Sakane, F.et al.: "EF-hand motifs of alpha-, beta-, gamma-isoforms of diacylglycerol kinase bind calcium with different affinities and conformational changes." Bichem.J.321. 59-64 (1997)
Yamada, K., Sakane, F.等人:“二酰基甘油激酶的 α-、β-、γ-异构体的 EF 手基序以不同的亲和力和构象变化结合钙。”
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加納英雄: "DGキナーゼ(二次メッセンジャーと産生酵素)" 廣川書店, 74-82 (1997)
嘉纳英雄:“DG 激酶(第二信使和产生的酶)”广川书店,74-82(1997)
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Fitzgibbon, J.: "Localisation of the gene encoding diacylglycerol kinase-3(DAGK-3)to human chromosome 3q27-28 and mouse chromosome 16" Curr.Oye Res.14. 1041-1043 (1995)
Fitzgibbon, J.:“编码二酰基甘油激酶 3 (DAGK-3) 的基因定位于人类染色体 3q27-28 和小鼠染色体 16”Curr.Oye Res.14。
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共 19 条
Discovery and analysis of dovel lipid signal transduction complexes
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批准号:18590274
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.49万
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财政年份:2006
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负责人:KANOH Hideo
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依托单位:
Intracellular and extracellular metabolism of lipid mediators- DGK and LPA
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批准号:16590234
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.24万
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财政年份:2004
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负责人:KANOH Hideo
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依托单位:
Studies on regulatory mechanisms of phospholipid-metabolizing enzymes
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批准号:10470035
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$7.1万
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财政年份:1998
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负责人:KANOH Hideo
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依托单位:
The function of DG kinase, a lipid phosphorylating enzyme having both EF-hands and zinc fingers.
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批准号:02454157
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$0.51万
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财政年份:1990
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负责人:KANOH Hideo
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依托单位:
Physiological function of lipid-phosphorylating enzymes and their mechanism of action.
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批准号:61570119
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.28万
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财政年份:1986
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负责人:KANOH Hideo
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依托单位:
海外基金