Molecular analysis of enzymes involved in animal phospholipid biosynthesis

参与动物磷脂生物合成的酶的分子分析

• 批准号: 07457044
• 负责人: KANOH Hideo
• 金额: $ 4.1万
• 依托单位: Sapporo Medical University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457044/
• 关键词: Diacylglycerol kinase; Phosphatidic acid phosphatase; signal transduction; Phospholipids; cDNA cloning; cDNAクローニング; 細胞分化; プレクストリン相同部位; 亜鉛フィンガー; EF-ハンド

We have been working on diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP). We succeeded for the first time in the cDNA cloning of mouse 35-kDa PAP,and subsequently cloned two human PAP isozymes designated PAP-2a and 2b.Our work showed that PAP is unexpectedly involved in the regulation of germ cell migration and cellular differentiation.We also described the cDNA cloning of DGK-delta. We analyzed the translocation of DGK-alpha upon T-lymphocyte activation, and also identified the catalytic domain of this DGK isozyme. We investigated the calcium-binding properties of EF-hand motifs present in common in alpha-, beta- and gamma isoforms of DGK.

我们一直在研究甘油二酯激酶（DGK）和磷脂酸磷酸酶（PAP）。我们首次成功地克隆了小鼠35-kDa PAP的cDNA，随后又克隆了两种人PAP同工酶PAP-2a和2b。我们的工作表明PAP与生殖细胞的迁移和细胞分化有关。我们还克隆了DGK-delta的cDNA。我们分析了T淋巴细胞活化后DGK-alpha的易位，并确定了这种DGK同工酶的催化结构域。我们研究了钙结合特性的EF-手图案共同存在于α，β和γ亚型的DGK。

项目成果

Sakane.F.: "Molecular cloning of a diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal teil similar to those of the EPH tamily of protgaintyrosino kinases." J.Biol.Chem.(印刷中).

Sakane.F.：“二酰基甘油激酶同工酶的分子克隆，其具有与原酪氨酸激酶的 EPH 家族相似的 pleckstrin 同源结构域和 C 端 teil（正在出版）。”

Kanoh,H.(共同執筆): "Oxford University press.London,U.K.(印刷中)" Diacylglycerol kinase in “Guide book on calcium binding proteins.

Kanoh, H.（合著者）：“牛津大学出版社。英国伦敦（正在印刷中）”《钙结合蛋白指南》中的二酰基甘油激酶。

Yamada, K., Sakane, F.et al.: "EF-hand motifs of alpha-, beta-, gamma-isoforms of diacylglycerol kinase bind calcium with different affinities and conformational changes." Bichem.J.321. 59-64 (1997)

Yamada, K., Sakane, F.等人：“二酰基甘油激酶的 α-、β-、γ-异构体的 EF 手基序以不同的亲和力和构象变化结合钙。”

Fitzgibbon, J.: "Localisation of the gene encoding diacylglycerol kinase-3(DAGK-3)to human chromosome 3q27-28 and mouse chromosome 16" Curr.Oye Res.14. 1041-1043 (1995)

Fitzgibbon, J.：“编码二酰基甘油激酶 3 (DAGK-3) 的基因定位于人类染色体 3q27-28 和小鼠染色体 16”Curr.Oye Res.14。

Yamada,Keiko: "EF-hand motifs of α-,β-,and γ-isofovms of diacylqlycerol kinase Beind calcium with different affinities and conformational changes" Biochem.J.321. 59-64 (1997)

Yamada, Keiko：“具有不同亲和力和构象变化的二酰甘油激酶 Beind 的 α-、β- 和 γ-isofovm 的 EF-hand 基序”Biochem.J.321 (1997)。