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Molecular epidemiological analysis of the antigen diversity of Plasmodium vivax and P.falciparum

间日疟原虫和恶性疟原虫抗原多样性的分子流行病学分析

基本信息

批准号：
07457069
负责人：
TANABE Kazuyuki
金额：
$ 5.18万
依托单位：
Osaka Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457069/
关键词：
Malaria Plasmodium Gene Antigen diversity MSP1 PCR TRAP Gene polymorphism 多様性

项目摘要

The diversity of surface antigens of Plasmodium vivax and P.falciparum was studied at the genetic on level field isolates from endemic areas of Thailand and Vietnam. Analysis of more than 300 isolates by PCR and DNA sequencing revealed the followings.1.Polymorphism of the merozoite surface antigen (MSP1) gene of P.vivax : 15 Thai isolates possessed 35 permutations in the regions from ICB5 to ICB6, most of which were dimorphic substitutions. Two types of recombination bwtween type 1 and type 2 sequences were found in a polymorphic region. These results suggest that allelic diversity may be generated by recombination as found for the P.falciparum MSP1 gene.2.Polymorphism of the merozoite surface antigen (MSP1) gene of P.falciparum : A PCR system, which identifies up to 24 different MSP1 gene types per isolate, was developed by using primers specific for allelic type sequences. In total, 17 to 21 different MSP1 gene types were identified in Vietnam and Thailand, respectively. Frequency distribution of MSP1 gene type differed significantly between the two areas. The F st analysis indicated significant divergence in variable blocks of MSP1 except block 2 between Thai and Vietnam isolates. Linkage analysis between variable blocks localized regions in the gene for frequent recombination (block 4) and less frequent recombination (blocks 3 and 5) as well as no recombination (blocks 6-16). Furthermore, Thai isolates showed 2 new polymorphic residues in the C terminus, 19 kD region of MSP1.3.Sequencing of the thrombospondin-related anonymous protein (TRAP) gene of P.falciparum of 20 Thai isolates showed variations specific for Thai isolates and also suggested recombination in the gene.

从遗传学水平上研究了来自泰国和越南流行区的间日疟原虫和恶性疟原虫的表面抗原多样性。结果表明：1.间日疟原虫裂殖子表面抗原（MSP 1）基因多态性：15株泰国分离株在ICB 5 ~ ICB 6区域内存在35个突变，其中多数为双态性替换。1型和2型序列在同一个多态区域内存在两种类型的重组。恶性疟原虫裂殖子表面抗原（MSP 1）基因的多态性：利用特异性引物建立了一种PCR扩增系统，可对每个分离株的MSP 1基因进行24种不同类型的鉴定。总共在越南和泰国分别鉴定了17至21种不同的MSP 1基因类型。MSP 1基因型的频率分布在两个地区之间存在显著差异。Fst分析表明，泰国和越南分离物之间的MSP 1的可变区，除了块2显着分歧。可变区之间的连锁分析定位于基因中频繁重组（区4）和较不频繁重组（区3和5）以及无重组（区6-16）的区域。此外，泰国分离株在MSP 1.3的C端19 kD区域发现2个新的多态性位点。对20株泰国分离株的TRAP基因进行测序，发现泰国分离株的TRAP基因发生了特异性变异，并提示该基因发生了重组。