The Function and Abnormality of myosin phosphatase in cardiovascular system
肌球蛋白磷酸酶在心血管系统中的功能及异常
基本信息
- 批准号:07457168
- 负责人:
- 金额:$ 4.35万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The function and abnormality of myosin phosphatase in cardiovascular system has been characterized in some detail and the following results have been presented.1.Signal transduction mechanism through myosin phospnatase (MP) in smooth muscle Rho-kinase phosphorylated the regulatory subunit (MYPT) of MP and inactivated the holoenzyme in vitro. Constitutively active Rho-kinase can induce contraction in Triton-X-100-permeabilized smooth muscle of rabbit portal vein. This contraction was accompanied by proportional increases in monophosphorylated myosin light chain. From above results it seems that MP and Rho-kinase are the important factors responsible for the regulation of smooth muscle tone.2.Identification of two human MYPT isoformsWe have identified two novel isoforms of MYPT of MP in human. MYPT1 isoform is quite similar to rat or chicken isoforms and show>80% sequence identity. MYPT1 is mainly located in various smooth muscle tissues. The gene of MYPT1 was found on chromosome 12 (12q15-q21.2). On the other hand, MYPT2 is found in heart and brain.and its gene was localized on chromosome 1 (1q32.1). Recent study shows that chromosome 1q32.1 contains the putative genes responsible for cardiomyopathy. Therefore we suggest that MYPT2 may be related to the occurrence of cardiomyopathy. With regard to this, further studies will be needed.3.Interaction of MP and phospholipidsThe interaction of MP with membrane components, i.e.various phospholipids was examined. The C-terminal part of MYPT was found to interact the acidic phospholipids. It was further shown that MYPT was phosphorylated by PKA and that phosphorylation of the MP holoenzyme decreased phospholipid binding with a recovery of phosphatase activity. These results support the idea that MP may interact with membranes and that phosphorylation by PKA could modify this interaction.
本文对肌球蛋白磷酸酶在心血管系统中的功能和异常进行了较为详细的研究,并获得了以下结果:1.平滑肌中肌球蛋白磷酸酶(myosin phosphnatase,MP)的信号转导机制:Rho激酶在体外磷酸化MP的调节亚基(myosin phosphnatase,MYPT),使MP的全酶失活。在Triton-X-100透性化的兔门静脉平滑肌中,组成性活性Rho激酶可引起收缩。这种收缩伴随着单磷酸化肌球蛋白轻链的成比例增加。以上结果表明,MP和Rho激酶是调节平滑肌张力的重要因子。2.两种人MYPT亚型的鉴定我们在人中鉴定了两种新的MP MYPT亚型。MYPT 1同种型与大鼠或鸡同种型非常相似,并且显示>80%的序列同一性。MYPT 1主要位于各种平滑肌组织中。MYPT 1基因定位于12号染色体(12 q15-q21.2)。MYPT 2基因定位于1号染色体(1q32.1)上,主要分布于心脏和脑组织。最近的研究表明,染色体1q32.1含有心肌病的致病基因。因此,我们认为MYPT 2可能与心肌病的发生有关。关于这一点,还需要进一步的研究。3. MP与磷脂的相互作用研究了MP与膜组分,即各种磷脂的相互作用。发现MYPT的C-末端部分与酸性磷脂相互作用。进一步表明MYPT被PKA磷酸化,MP全酶的磷酸化降低了磷脂结合,磷酸酶活性恢复。这些结果支持MP可能与膜相互作用的想法,PKA的磷酸化可以改变这种相互作用。
项目成果
期刊论文数量(34)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takeshi Matsui: "Rho-associated kinase,a novel serine/threonine kinase,as a putative target for small GTP-binding protein Rho" The EMBO Journal. 15. 2208-2216 (1996)
Takeshi Matsui:“Rho 相关激酶,一种新型丝氨酸/苏氨酸激酶,作为小 GTP 结合蛋白 Rho 的推定靶点”EMBO 杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Takeshi Matsui, Mutsuki Amano, Takaharu Yamamoto, Kazuyasu Chihara, Masato Nakafuku, Masaaki Ito, Takeshi Nakano, Katsuya Okawa, Akihiro Iwamatsu and Kozo Kaibuchi: "Rhoassociated kinase, a novel serine/threonine kinase, as a putative target for small GTP
Takeshi Matsui、Mutsuki Amano、Takaharu Yamamoto、Kazuyasu Chihara、Masato Nakafuku、Masaaki Ito、Takeshi Nakano、Katsuya Okawa、Akihiro Iwamatsu 和 Kozo Kaibuchi:“Rho 相关激酶,一种新型丝氨酸/苏氨酸激酶,作为小 GTP 的推定靶标
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Katsuya Hirano: "Interaction of the ribosonal protein, LS, with proteinphosphatase type1." Journal of Biological Chemistry. 270. 19786-19790 (1995)
Katsuya Hirano:“核糖蛋白 LS 与 1 型蛋白磷酸酶的相互作用。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kazuhito Ichikawa: "Interactions and Properties of the smooth muscle myosin phosphatase" Biochemistry. 35. 6313-6320 (1996)
Kazuhito Ichikawa:“平滑肌肌球蛋白磷酸酶的相互作用和特性”生物化学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masaki Fujioka, Nobuaki Takahashi, Hideharu Odai, Shigemasa Araki, Kazuhito Ichikawa, Jianhua Feng, Mamoo Nakamura, Kozo Kaibuchi, David J.Hartshorne, Takeshi Nakano and Masaaki Ito: "Identification of a second gene encoding the target subunit of myosin p
Masaki Fujioka、Nobuaki Takahashi、Hideharu Odai、Shigemasa Araki、Kazuhito Ichikawa、Jianhua Feng、Mamoo Nakamura、Kozo Kaibuchi、David J.Hartshorne、Takeshi Nakano 和 Masaaki Ito:“编码肌球蛋白 p 靶亚基的第二个基因的鉴定
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
NAKANO Takeshi其他文献
NAKANO Takeshi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('NAKANO Takeshi', 18)}}的其他基金
Reform of the Civil Code and Theory of Administrative Law
民法典改革与行政法理论
- 批准号:
26380032 - 财政年份:2014
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Theoretical Analysis of Legislative Drafting by Cabinet Legislative Bureau
内阁法制局立法起草的理论分析
- 批准号:
22730013 - 财政年份:2010
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Comprehensive Research on Japanese Substantive Administrative Law-focused on the standing of the two types of injunctive actions
日本实体行政法综合研究——以两类禁令行为为中心
- 批准号:
18730015 - 财政年份:2006
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Molecular mechanism of plant of development regulation by progesterone
黄体酮调控植物发育的分子机制
- 批准号:
18580100 - 财政年份:2006
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analyses of the functions of MYPT family, novel anchoring proteins, in cardiovascular system by their knockout and transgenic mice
通过敲除小鼠和转基因小鼠分析新型锚定蛋白 MYPT 家族在心血管系统中的功能
- 批准号:
12470152 - 财政年份:2000
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulatory Mechanisms of Smooth Muscle Contraction
平滑肌收缩的调节机制
- 批准号:
08044269 - 财政年份:1996
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for international Scientific Research
Analysis of molecular mechanism in Ca^<2+>-independent vasocontraction
Ca^2非依赖性血管收缩的分子机制分析
- 批准号:
03454252 - 财政年份:1991
- 资助金额:
$ 4.35万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
Estrogen, Myosin Phosphorylation and Muscle Thermogenesis
雌激素、肌球蛋白磷酸化和肌肉生热作用
- 批准号:
RGPIN-2019-04339 - 财政年份:2022
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
Estrogen, Myosin Phosphorylation and Muscle Thermogenesis
雌激素、肌球蛋白磷酸化和肌肉生热作用
- 批准号:
RGPIN-2019-04339 - 财政年份:2021
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
Estrogen, Myosin Phosphorylation and Muscle Thermogenesis
雌激素、肌球蛋白磷酸化和肌肉生热作用
- 批准号:
RGPIN-2019-04339 - 财政年份:2020
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
Estrogen, Myosin Phosphorylation and Muscle Thermogenesis
雌激素、肌球蛋白磷酸化和肌肉生热作用
- 批准号:
RGPIN-2019-04339 - 财政年份:2019
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
The influence of myosin phosphorylation on mechanical and metabolical properties of fast twitch skeletal muscle
肌球蛋白磷酸化对快肌骨骼肌机械和代谢特性的影响
- 批准号:
312012-2008 - 财政年份:2012
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
The influence of myosin phosphorylation on mechanical and metabolical properties of fast twitch skeletal muscle
肌球蛋白磷酸化对快肌骨骼肌机械和代谢特性的影响
- 批准号:
312012-2008 - 财政年份:2011
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
The influence of myosin phosphorylation on mechanical and metabolical properties of fast twitch skeletal muscle
肌球蛋白磷酸化对快肌骨骼肌机械和代谢特性的影响
- 批准号:
312012-2008 - 财政年份:2010
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
The influence of myosin phosphorylation on mechanical and metabolical properties of fast twitch skeletal muscle
肌球蛋白磷酸化对快肌骨骼肌机械和代谢特性的影响
- 批准号:
312012-2008 - 财政年份:2009
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
The influence of myosin phosphorylation on mechanical and metabolical properties of fast twitch skeletal muscle
肌球蛋白磷酸化对快肌骨骼肌机械和代谢特性的影响
- 批准号:
312012-2008 - 财政年份:2008
- 资助金额:
$ 4.35万 - 项目类别:
Discovery Grants Program - Individual
Regulation of Myosin Phosphorylation in Smooth Muscle
平滑肌肌球蛋白磷酸化的调节
- 批准号:
6719089 - 财政年份:2003
- 资助金额:
$ 4.35万 - 项目类别: