Attempts to immortalize vascular smooth muscle cells for the elucidation of the pathogenesis of moyamoya disease

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Although the primary lesion of moyamoya disease is stenosis of the intracranial arterial trunk, the superficial temporal artery (STA) has been suggested to be involved in moyamoya disease. Immortalization of vascular smooth muscle cells derived from the STA,which was obtained at surgery, was attempted using LINXv-myc retrovirus in order to obtain enough material for molecular biological studies. Although smooth muscle cells infected with the LINXv-myc retrovirus has not been immortalized, they were able to be cultured several times longer than original cells.