Involvement of L-arginine-nitric oxide pathway in cerebral vasospasm

L-精氨酸-一氧化氮途径参与脑血管痉挛

• 批准号: 05454396
• 负责人: KIKUCHI Haruhiko
• 金额: $ 4.42万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454396/
• 关键词: Cerebral Vasospasm; Subarachnoid Hemorrhage; Nitric Oxide; 一酸化窒素合成酵素

Although cerebral vasospasm is one of the major complications after subarachnoid hemorrhage, its pathogenesis remains to be elucidated. Cerebral circulation is regulated by vasoactive substances released from vasoactive substances such as EDRF (i.e.NO) , endothelin, and also by neurotransmitters from perivascular nerve fibers such as CGRP,VIP,SP,NO.We investigated in the present study the possible involvement of NO in the occurrence of cerebral vasospasm after subarachnoid hemorrhage. Experimental subarachnoid hemorrhage was produced in rats and dogs by injecting autologous arterial blood into the cistema magna.Severe vasospasm was obtained in basilar arteries on Day 1 in rats and Day 3 in dogs. Immunopositive materials for NO synthase in the endothelium and in the adventitial nerve fibers of major cerebral arteries were reduced after subarachnoid hemorrhage, and NO mediated endothelium dependent vasorelaxations and NO mediated neurogenic vasorelaxations were impaired after subarachnoid hemorrhage. These results indicated that the impairement of NO mediated vasorelaxation mechanisms might be involved in the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage.

脑血管痉挛是蛛网膜下腔出血后的主要并发症之一，其发病机制尚不清楚。脑循环受EDRF（即NO）、内皮素等血管活性物质释放的血管活性物质以及血管周围神经纤维的CGRP、VIP、SP、NO等神经递质的调节。用自体动脉血注入枕大池造成大鼠和狗实验性蛛网膜下腔出血，大鼠第1天和狗第3天基底动脉出现严重的血管痉挛。蛛网膜下腔出血后，主要脑动脉内皮和外膜神经纤维中NO合酶的免疫阳性物质减少，并且NO介导的内皮依赖性血管舒张和NO介导的神经源性血管舒张在蛛网膜下腔出血后受损。提示NO介导的血管舒张机制受损可能参与了蛛网膜下腔出血后脑血管痉挛的发病机制。

项目成果

野崎 和彦: "脳血管壁NO合成酵素(NOS)含有神経線維" スパズムシンポ講演集,脳血管攣縮. 9. 16-23 (1993)

Kazuhiko Nozaki：“脑血管壁含 NO 合酶 (NOS) 的神经纤维”痉挛研讨会讲座，脑血管痉挛。 9. 16-23 (1993)

Kazuhiko Nozaki: "Pathogenesis of Cerebral Vasospasm" Neurosurgery Letters. Vol 3. 647-652 (1993)

野崎和彦：“脑血管痉挛的发病机制”神经外科快报。

野崎和彦: "脳血管壁NO合成酵素(NOS)含有神経線維-くも膜下出血後の変化について-" スパズムシンポ講演集. 9. (1993)

野崎和彦：“脑血管壁含 NOS 的神经纤维 - 蛛网膜下腔出血后的变化”痉挛研讨会讲座 9。（1993 年）

野崎和彦: "脳血管壁NO合成酵素(NOS)含有神経線維" スパズムシンポ講演集,脳血管攣縮. 9. 16-23 (1993)

Kazuhiko Nozaki：“脑血管壁含 NO 合酶 (NOS) 的神经纤维”痉挛研讨会讲座，脑血管痉挛。 9. 16-23 (1993)

野崎和彦: "脳循環調節におけるNOの役割" 血管. 17. 39-44 (1994)

Kazuhiko Nozaki：“NO 在脑循环调节中的作用”血管。 17. 39-44 (1994)