Bio-Engineering Approach to Mechanisms of Angiogenesis Induced by Blood Flow

血流诱导血管生成机制的生物工程方法

基本信息

批准号：
07457326
负责人：
ASATO Hirotaka
金额：
$ 3.46万
依托单位：
UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457326/
关键词：
Microcirculation Angiogenesis Blood flow Shear stress リモデリング血行力学的適応血管構築

项目摘要

The effect of the blood flow and flow-oriented wall shear stress in microvascular angiogenesis was studied in vivo, by employing the rabbit ear chamber technique to quantitatively assess the effect on angiogenesis of chronic administration of the alpha_1 brocker, prazosin, which increases peripheral blood flow. Rabbits were treated with prazosin hydrochloride (50 mg/l in water) orally after ear chambers were installed. The microcirculation in the chamber was observed and recorded from 4 to 23 postoperative day (POD) by an intravital videomicroscope. The relative area covered by vascularized tissue in the chamber (CA), the mean vascular density in the covered area (MD) and the relative vascular area (RA) were quantified. Wall shear stress in venules (20-40mum ID) was also estimated. CA at 7-17 POD,MD at 11-23 POD and RA at 9-23 POD were significantly increased in the prazosin-treated animals. The final RA in the treated group was approximately 21% larger than that in the control group. The measured level of wall shear stress, which was increased 1.8 times by prazosin to at 9 POD,gradually decreased to ward the control level until they met at 13 POD.These findings suggest that the chronichigh flow load enhances microvascular structural change during angiogenesis in vivo, indicating the same adaptive responses of microvessels to wall shearstress as of large vessels. This study has experimentally proven that angiogenesis during tissue repair is also regulated by shear stress due to blood flow.

通过使用兔耳室技术来定量评估alpha_1 brocker的长期给药的影响，在体内研究了微血管血管生成中血流和面向流量的壁剪应力在体内的影响。安装耳室后，用盐酸丙唑嗪（水中50 mg/L）对兔子进行治疗。观察到腔室中的微循环，并通过插入式视频显微镜从4到23日（POD）记录。量化了由腔室（CA）的血管组织覆盖的相对区域，覆盖面积（MD）和相对血管面积（RA）的平均血管密度。还估计了静脉（20-40MUM ID）中的壁剪应力。在prazosin治疗的动物中，在11-23 POD的7-17 POD，MD，在9-23 POD处的Ca显着增加。治疗组的最终RA大约比对照组大约21％。测得的壁剪应力水平，通过prazosin提高到9个POD，逐渐降低了控制水平，直到在13个POD处相遇为控制水平。这些发现表明，Chronichigh流量载荷在体内血管生成过程中增强了微血管结构变化，表明对壁shearspressers as and Ball shearspressers as and Ball shearspressers as and Ball shearspressers as and shearearspressers as and shearearspressers as and shearearspressels as and shearspressels as and shearsalss均具有。这项研究已在实验上证明，组织修复过程中的血管生成还受到血液流动引起的剪切应力的调节。