Blood flow influences vascular growth during tumor angiogenesis

血流影响肿瘤血管生成过程中的血管生长

• 批准号: 12670912
• 负责人: AKAGI Kiyoshi
• 金额: $ 2.18万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670912/
• 关键词: experimental tumor; Transparent chamber; neovascularization; ラット背皮弁観察窓; 腫瘍内微小血管; 放射線感受性

We observed growing tumor vessels in vivo to study the relationship between blood flow and vascular enlargement. Mammary adenocarcinoma was implanted into Fisher-344 rat with dorsal shin-fold transparent chambers. Vascular growth was observed and recorded on videotape through a microscope for 6h. Vascular networks were photographed and traced every 30min. To identify changes over time. Tumor sections were stained with Masson's trichrome and anti-Factor-8 related antigen. Tumor growth was rapid enough for differences to be seen each hour. Vessels with a high blood flow showed an increase in diameter within a few hours and new branches formed from these vessels. In contrast, vessels without an increase in blood flow showed no change in diameter. Vessels within the interstitium surrounding the tumor were lined by endothelium that was positive for anti-8 related antigen staining. Vessels in the tumor had extremely rare endothelial cells detectable by masson's trichrome or anti-8 related antigen staining. In conclusion, increased blood flow may cause vascular enlargement and some primitive vessels seem to lack endothelium.

我们在活体上观察肿瘤血管的生长，以研究血流与血管扩张的关系。将乳腺癌细胞植入Fisher-344大鼠背部胫褶透明腔。通过显微镜观察血管生长并在录像带上记录6 h。每30分钟对血管网进行拍照和追踪。确定随时间的变化。肿瘤切片用Masson三色和抗-因子-8相关抗原染色。肿瘤生长足够快，每小时都可以看到差异。具有高血流量的血管在几小时内显示出直径的增加，并且从这些血管形成新的分支。相比之下，血流没有增加的血管直径没有变化。肿瘤周围的血管内皮细胞内的血管内衬抗8相关抗原染色阳性。肿瘤内血管内皮细胞极少，可通过马森三色染色或抗8相关抗原染色检测到。总之，血流量增加可能导致血管扩张，一些原始血管似乎缺乏内皮。