Title of Project ; Analysis of differentiation of human luteal cells, using monoclonal antibodies that recognize differentiation-related antigens on luteal cells.

项目名称;

基本信息

  • 批准号:
    07457385
  • 负责人:
  • 金额:
    $ 4.35万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1996
  • 项目状态:
    已结题

项目摘要

Summary of Research ResultsCorpus luteum (CL) has important physiological functions in the female reproduction. It is generally accepted that corpus luteum is essential for successful pregnancy. Luteal dysfunction is assumed to be one of causes of infertility and/or habitual abortion.In addition, it is suggested that luteal insufficiency may cause endometrial hyperplasia and cancer. Furthermore, during the luteal phase, luteal cells show morphological and functional changes. During the CL formation, follicular cells differentiate to luteal cells, and steroidogenic enzymes are induced to produce progesterone and so on. During the CL regression, luteal cells demonstrate apoptotic changes. Therefore, luteal cells are useful materials for analysis of cell differentiation. However, differentiation of luteal cells are not understood, to date.To investigate the differentiation pathways and regulatory mechanisms of differentiation of human luteal cells in the ovary, we raised murine monoclonal … More antibodies (mAbs) against human luteal cells, and analyzed the cell-lineage-and differentiation stage-specifically expressed molecules on cell surface. (1) We have obtained several mAbs that recognize granulosa cell lineage-specific or thecal cell lineage-specific cell surface molecules. From these data, we proposed the human granulosa cells differentiate to large luteal cells of the menstrual CL and also CL of early pregnancy, and that human theca interna cells differentiate to small luteal cells. (2) We have found expression profiles of several molecules are different between the menstrual CL and CL of pregnancy. This suggest that CL of pregnancy have different functions from CL of the cycle. (3) Though the study on the effect of gonadotropins and cytokines on the expression of the differentiation-related antigens on luteal cells, we have found that differentiation of luteal cells are regulated not only by gonadotropins but also by cytokines. This suggests that immunological factors are involved in the differentiation of menstrual CL to CL of pregnancy. (4) We have found that the interaction between integrins (cell adhesion molecules) and extracellular matrices are local regulator of the differentiation of luteal cells. Less
研究结果总结黄体(CL)在女性生殖中具有重要的生理功能。人们普遍认为黄体对于成功怀孕至关重要。黄体功能不全被认为是不孕和/或习惯性流产的原因之一。此外,黄体功能不全可能导致子宫内膜增生和癌症。此外,在黄体期,黄体细胞表现出形态和功能的变化。在CL形成过程中,滤泡细胞分化为黄体细胞,并诱导类固醇生成酶产生黄体酮等。在 CL 回归过程中,黄体细胞表现出凋亡变化。因此,黄体细胞是分析细胞分化的有用材料。然而,迄今为止,黄体细胞的分化尚不清楚。为了研究卵巢中人黄体细胞的分化途径和分化调控机制,我们培养了针对人黄体细胞的鼠单克隆抗体(mAb),并分析了细胞表面的细胞谱系和分化阶段特异性表达的分子。 (1)我们已经获得了几种识别颗粒细胞谱系特异性或鞘细胞谱系特异性细胞表面分子的单克隆抗体。根据这些数据,我们提出人颗粒细胞分化为月经CL和早孕CL的大黄体细胞,人卵泡膜内细胞分化为小黄体细胞。 (2)我们发现一些分子的表达谱在月经CL和妊娠CL之间是不同的。这表明妊娠期CL与周期CL具有不同的功能。 (3)通过研究促性腺激素和细胞因子对黄体细胞分化相关抗原表达的影响,我们发现黄体细胞的分化不仅受促性腺激素的调节,还受到细胞因子的调节。这表明免疫因素参与了经期 CL 与妊娠 CL 的分化。 (4)我们发现整合素(细胞粘附分子)与细胞外基质之间的相互作用是黄体细胞分化的局部调节因子。较少的

项目成果

期刊论文数量(27)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakamura K et al.: "An aminopeptidase inhibitor,bastatin,enhances gonadotropin-stimulated ovulatioon in mice." Human Reproduction. 11. 1952-1957 (1996)
Nakamura K 等人:“一种氨肽酶抑制剂,巴他汀,可增强促性腺激素刺激的小鼠排卵。”
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    0
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Fujiwara H et al.: "A differentiation antigen of human large luteal cells in corpora lutea of the menstrual cycle and early pregnancy." Biology of Reproduction. 54. 1173-1183 (1996)
Fujiwara H 等人:“月经周期和妊娠早期黄体中人类大黄体细胞的分化抗原。”
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    0
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Hujiwara H et al.: "Granulosa cells express integrin a6 : possible involvement of integrin a6 on folliculogenesis." Hormone Research. 46(suppl1). 24-30 (1996)
Hujiwara H 等人:“颗粒细胞表达整合素 a6:整合素 a6 可能参与卵泡发生。”
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    0
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Fujiwara H, et al.: "A differentiation antigen of human large luteal cells in corporalutea of the menstrual cycle and early pregnancy." Biology of Reproduction. (in press). (1996)
Fujiwara H 等人:“月经周期和妊娠早期黄体中人类大黄体细胞的分化抗原。”
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
Nakamura K,et al.: "An aminopeptidase inhibitor, bestatin, enhances gonadotropin-stimulated ovulation in mice" Hum.Reprod.11. 1952-1957 (1996)
Nakamura K 等人:“氨基肽酶抑制剂贝他汀可增强小鼠促性腺激素刺激的排卵”Hum.Reprod.11。
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    0
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MORI Takahide其他文献

MORI Takahide的其他文献

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{{ truncateString('MORI Takahide', 18)}}的其他基金

Endocrinological and immunological study for local regulation of ovarian function
卵巢功能局部调节的内分泌学和免疫学研究
  • 批准号:
    05454446
  • 财政年份:
    1993
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
The role of cytokines in reprodution and embryogenesis
细胞因子在生殖和胚胎发生中的作用
  • 批准号:
    05304039
  • 财政年份:
    1993
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Co-operative Research (A)
A basic and clinical study on regulatory mechanism operating for the function of human corpus luteum
人黄体功能调控机制的基础与临床研究
  • 批准号:
    61480350
  • 财政年份:
    1986
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
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