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A basic and clinical study on regulatory mechanism operating for the function of human corpus luteum

人黄体功能调控机制的基础与临床研究

基本信息

批准号：
61480350
负责人：
MORI Takahide
金额：
$ 4.42万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480350/
关键词：
Regulatory mechanism of human corpora lutea function Porcirne granulosa cells in culturr Interleukin-1 FSH Human corpora lutea L cells ヒト顆粒膜黄体細胞ヒト莢膜黄体細胞ヒト黄体機能調節機構 LH-RH静脈内投与黄体機能不全莢膜黄体細胞顆粒膜黄体細胞ゴナドトロピン

项目摘要

The primary object of this study was to elucidate the mechanism through which the function of human corpora lutea, namely the production of steroids, is regulated under the physiologic condition, thereby providing a physiologic basis for our better understanding of luteal phase defect, an important but poorly understood cause of infertility. Using both in vitro and in vivo methods, we were able to disclose hitherto unknown important aspects of the regulatory mechanism of human corpora lutea. Firstly, we have shown that human corpora lutea are consisted of two types of cells, namely L and S cells, which are functionally analogous to granulosa and thecal cells of ovarian follicles, respectively. L cells are twice as potent in the production of progesterone and in the aromatase activity as S cells, whereas the reverse is true as regards the production of androgens. Reactivity to hCG, as manifested by an enhanced production of androgens and progesterone, occurs only in S cells. Whereas bot … More h L and S cells are capable of aromatizing androgens, FSH-induced stimulation of estrogen production under the presence of androgen substrate was elicited only in L cells. It is therefore proposed that co-oparative interraction between the two types of luteal cells of human copora lutea is operative under the control of two different gonadoropins. Secondly, using porcine granulosa cells obtained from medium-sized follicles, we have for the first time shown that basal as well as LH-stimulated production of progesterone is inhibited by interleukin-1. Morphological lutenization of these cells under th presence of LH was also inhibited by this lymphokine. In in vivo studies, we have shown that circulating levels of estradiol are elevated by the administration of FSH into nomal women in their luteal phase, thereby providing evidence for the possible participation of FSH in the regulation of corpus luteal function. These results from in vivo studies consort with those from in vitro studies described above. In summary, the results from this project do contribute significantly to the understanding of the regulatory mechanism of human corpora lutea function. Less

本研究的主要目的是阐明在生理条件下调节人类黄体功能（即类固醇产生）的机制，从而为我们更好地理解黄体功能障碍提供生理学基础，黄体功能障碍是不孕症的重要原因，但人们对此了解甚少。使用在体外和体内的方法，我们能够公开迄今未知的重要方面的人类黄体的调节机制。首先，我们已经证明，人类黄体是由两种类型的细胞，即L和S细胞，这是功能类似的颗粒和卵泡膜细胞，分别。L细胞在孕酮的产生和芳香化酶活性方面是S细胞的两倍，而在雄激素的产生方面则相反。对hCG的反应性，表现为雄激素和孕酮的产生增加，仅发生在S细胞中。而bot ...更多信息 L细胞和S细胞都能芳香化雄激素，FSH诱导的雌激素产生的刺激下，雄激素底物的存在下，只引起L细胞。因此，我们认为人黄体母细胞的两种类型的黄体细胞之间的协同作用是在两种不同的性腺激素的控制下进行的。其次，使用从中等大小的卵泡获得的猪颗粒细胞，我们首次表明，基础以及LH刺激的孕酮的产生被白细胞介素-1抑制。这种淋巴因子还能抑制LH存在下这些细胞的形态学Lutenization。在体内研究中，我们已经表明，在正常妇女的黄体期给予FSH可提高雌二醇的循环水平，从而为FSH可能参与调节黄体功能提供了证据。这些体内研究结果与上述体外研究结果一致。综上所述，本研究的结果对于了解人类黄体功能的调控机制有重要的贡献。少