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Procrsses of degeneration and regeneration of retinal ganglion cells of the mammalian retina after axonal injury

哺乳动物视网膜轴突损伤后视网膜神经节细胞的变性与再生过程

基本信息

批准号：
07457415
负责人：
TAUCHI Masaki
金额：
$ 2.3万
依托单位：
Okayama Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457415/
关键词：
retina retinal ganglion cells Confocal laser microscope optic nerve axonal regeneration electron microscope neuron nutritional factor

项目摘要

We studied the three-dimensional dendritic morphology, synaptic structure and the effects of nutritional factors on the axotomized retinal ganglion cells (RCGs) in the adult rat retina. The animals of 90 days old were axotomized right behind the eye and left about 30 days for the experiments. To observe dendritic shape 3-dimensionally, lucifer dye was injected intracellularly to the RCGs retrogradely-labeled with 4SPdiI and such specimens were subjected to analyze by using laser scanning confocal microscope. For the electron microscopic study, photooxidation of lucifer or neurobiotin injection metod were used. Various neurotrophic factors were injected intraocularly and the effects were measured by counting the survival of the retinal ganglion cells. By the experiments, some results described below were obtained. 1) the survived retinal ganglion cells after axotomy for a long period of time exhibit larger soma diameter more than 20 um and their dendritic shape implied them as alpha ganglion cell origin. 2) Most of the ganglion cells survived showed larger dendritic tree length as much as 20% of that of normal alpha ganglion cells locating in the same retinal eccentricity. 3) Neurobiotin injection revealed that many of the injected axtomized ganglion cells show tracer-coupling and the coupling was mainly occurred in the neighboring amacrine cells. 4) The ganglion cells long survived were found to have gap junction between certain cell types either in the inner nuclear layr or ganglion cell layr. 5) Some neurotrophic factors were effective to accelerate axonal outgrowth but did not increase survival ratio of RGC after axotomy.

我们研究了成年大鼠视网膜轴突切断后视网膜神经节细胞（RCG）的三维树枝状形态、突触结构以及营养因素的影响。将90日龄的动物在眼后右侧进行轴突切断，并留下约30天用于实验。为了观察树突状细胞的三维形态，将荧光染料注射到用4SPDiI逆行标记的RCG细胞内，并使用激光扫描共聚焦显微镜对这些标本进行分析。电镜观察采用荧光素光氧化法或神经生物素注射法。眼内注射各种神经营养因子，通过计数视网膜神经节细胞的存活来测量效果。通过实验，获得了以下描述的一些结果。1)轴突切断后长时间存活的视网膜神经节细胞表现出大于20 μ m的较大索马直径，并且它们的树突形状暗示它们是α神经节细胞起源。2)大多数存活的神经节细胞显示出更大的树突树长度高达20%的正常α神经节细胞位于同一视网膜偏心。3)注射神经生物素后，许多神经节细胞显示示踪剂偶联，且偶联主要发生在邻近的无长突细胞。4)长期存活的神经节细胞在内核层或神经节细胞层中存在某些细胞类型之间的缝隙连接。5)某些神经营养因子能促进轴突生长，但不能提高RGC的存活率。