权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Immunolocalization of biosynthetic and metablic enzymes of catecholamine in human dental pulp.

人牙髓中儿茶酚胺生物合成和代谢酶的免疫定位。

基本信息

批准号：
07457435
负责人：
INOUE Katsuhiro
金额：
$ 4.03万
依托单位：
Matsumoto Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457435/
关键词：
catecholamine enzyme dopa dental pulp human 神経免疫組織化学

项目摘要

The relatively large amounts of DOPA as compared with the concentration of norepinephrine are found in human dental pulp. Local production of catecholamines in the human dental pulp was studied by immunocytochemical demonstration of tyrosine hydroxylase (TH), L-aromatic amino acid decarboxylase (AADC), dopamine-beta-hydroxylase (DBH) phenylethanolamine-N-methyltransferase (PNMT), the enzymes catalyzing the formation of DOPA,dopamine, norepinephrine and epinephrine, respectively. A rich innervation of TH- and AADC-immunoreactive nerve fibers was seen around the arterioles. The fine beaded immunoreactive varicosities of the DBH were found on the wall of arterioles. TH-immunoreactive nerve fibers were also observed in the cell rich zone. No specific PNMT immunoreactivity was observed. The results suggest that some norve fibers in the human dental pulp may synthesize norepinephrine as an end-product of catecholamine biosynthesis. AADC and COMT are localized in blood vessel walls of human dental pulp. This localization suggests a functional relationship between COMT and AADC with regard to the metabolism of DOPA.The endothelial cells of the arteriole contained COMT.This results suggest an enzymatic barrier to catecholamines ; a possible blood-dental pulp barrier. COMT activity in endothelial cells of vascular wall, by providing a means for the inactivation of catechols, may be an important mechanism for neurohormonal regulation of the blood flow and blood pressure under normal conditions. AADC was present in the smooth muscle cells of the arteriole. These smooth muscle cells of the arteriole may play a role like APUD cells.

与去甲肾上腺素的浓度相比，在人牙髓中发现相对大量的多巴。本文用免疫细胞化学方法研究了人牙髓局部儿茶酚胺的产生，包括酪氨酸羟化酶（TH）、L-芳香族氨基酸脱羧酶（AADC）、多巴胺-β-羟化酶（DBH）、苯乙醇胺-N-甲基转移酶（PNMT），这些酶分别催化多巴、多巴胺、去甲肾上腺素和肾上腺素的形成。小动脉周围有丰富的TH和AADC免疫反应阳性神经纤维分布。在小动脉壁上可见细小的串珠状静脉曲张。TH免疫反应阳性神经纤维也观察到的细胞丰富的区域。未观察到特异性PNMT免疫反应性。结果提示，人牙髓中的某些神经纤维可能合成去甲肾上腺素作为儿茶酚胺生物合成的终产物。AADC和COMT定位于人牙髓的血管壁中。这一定位表明COMT和AADC之间的DOPA代谢的功能关系。微动脉的内皮细胞含有COMT。这一结果表明，儿茶酚胺的酶屏障，一个可能的血牙髓屏障。血管壁内皮细胞中的COMT活性通过提供儿茶酚失活的手段，可能是正常条件下神经激素调节血流和血压的重要机制。AADC存在于小动脉的平滑肌细胞中。这些小动脉的平滑肌细胞可能发挥类似APUD细胞的作用。