Etiological study of oral disease in HIV and HTLV-I positive patients -characteristics of salivary gland cells-

本研究从1992 ~ 1996年九州大学齿科医院口腔颌面外科第二科收治的103例血清学检测为HTLV-I阳性的干燥综合征（SS）患者中，选取11例进行了研究，明确了HTLV-I与SS发病机制的关系。聚合酶链反应（PCR）扩增的细胞DNA显示，从唇唾液腺（SG）提取的细胞DNA中含有完整的HTLV-Ⅰ型前病毒DNA的SG。在SG的切片中检测HTLV-Ⅰ型前病毒DNA的原位PCR杂交表明，HTLV-Ⅰ型前病毒DNA在浸润的T细胞的核中，而不是在唾液腺上皮细胞或腺泡细胞。使用MT 2细胞、人HTLV-I感染的T细胞系和唾液腺上皮细胞系进行HTLV-I前病毒DNA的定量，结果显示SG中的病毒载量比外周血单核细胞中的病毒载量高约8倍至9 × 104倍，因此提示HTLV-I感染的T细胞在SG中蓄积。HTLV-I可能导致自身反应性T细胞增殖，结果，随后诱导SS。

The involvement of Human T lymphotrophic virus type I (HTLV-I) in the pathogenesis of Sjogren' syndrome (SS), which is also seen in HIV-infected patients, was clarified in this study.Eleven of one hundred and three SS patients, who were serologically HTLV-I positive, referred to the Second Department of Oral and Maxillofacial Surgery, Kyushu University Dental Hospital between 1992 and 1996, were studied. A polymerase chain reaction (PCR) amplification of cellular DNA revealed that the cellular DNA extracted from the labial salivary glands (SG) contained full HTLV-I proviral DNA in the SG.In situ PCR hybridization which detect HTLV-I proviral DNA in the section of the SG demonstrated HTLV-I proviral DNA in the nucleus of the infiltrating T cells, but not in either salivary gland epithelial or acinar cells. Quantification of HTLV-I proviral DNA was performed using MT2 cells, a human HTLV-I -infected T cell line and salivary gland epithelial cell lines, and revealed that the viral loads in the SG were approximately 8 times to 9 * 10^4 times higher than those in the peripheral blood mononuclear cells.An accumulation of HTLV-I-infected T cells in the SG is thus suggested. HTLV-I likely cause the self-reactive T cells proliferate and, as a result, thereafter induced SS.