Etiological study of oral disease in HIV and HTLV-I positive patients -characteristics of salivary gland cells-

HIV和HTLV-I阳性患者口腔疾病的病因学研究-唾液腺细胞的特征-

• 批准号: 07457492
• 负责人: ABE Kihachiro
• 金额: $ 4.99万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457492/
• 关键词: HIV; HTLV-I; salivary gland; cytokine; T cell; Sjogren's syndrome; autoimmune disease; HTLV-1

The involvement of Human T lymphotrophic virus type I (HTLV-I) in the pathogenesis of Sjogren' syndrome (SS), which is also seen in HIV-infected patients, was clarified in this study.Eleven of one hundred and three SS patients, who were serologically HTLV-I positive, referred to the Second Department of Oral and Maxillofacial Surgery, Kyushu University Dental Hospital between 1992 and 1996, were studied. A polymerase chain reaction (PCR) amplification of cellular DNA revealed that the cellular DNA extracted from the labial salivary glands (SG) contained full HTLV-I proviral DNA in the SG.In situ PCR hybridization which detect HTLV-I proviral DNA in the section of the SG demonstrated HTLV-I proviral DNA in the nucleus of the infiltrating T cells, but not in either salivary gland epithelial or acinar cells. Quantification of HTLV-I proviral DNA was performed using MT2 cells, a human HTLV-I -infected T cell line and salivary gland epithelial cell lines, and revealed that the viral loads in the SG were approximately 8 times to 9 * 10^4 times higher than those in the peripheral blood mononuclear cells.An accumulation of HTLV-I-infected T cells in the SG is thus suggested. HTLV-I likely cause the self-reactive T cells proliferate and, as a result, thereafter induced SS.

为明确人类T淋巴细胞营养病毒I型(HTLV-I)在干燥综合征(SS)发病机制中的作用，对1992年至1996年间在九州大学口腔医院口腔颌面外科二科就诊的103例血清HTLV-I阳性的SS患者进行了研究。细胞DNA的聚合酶链式反应(PCR)扩增显示，从嘴唇唾液腺(SG)中提取的细胞DNA中含有完整的HTLV-I前病毒DNA。原位PCR杂交检测SG切片中的HTLV-I前病毒DNA表明，HTLV-I前病毒DNA存在于浸润性T细胞的细胞核中，而在涎腺上皮细胞和腺泡细胞中均未见。用MT2细胞、人HTLV-I感染的T细胞系和涎腺上皮细胞系对HTLV-I前病毒DNA进行了定量，结果显示SG中的病毒载量大约是外周血单核细胞的8倍至9×104倍，这表明感染HTLV-I的T细胞在SG中积聚。HTLV-I可能导致自身反应性T细胞增殖，从而诱发SS。

项目成果

Y.Ohyama, S.Nakamura, H.Hara, M.Shinohara, M.Sasaki, A.Ikebe-Hiroki, T.Mouri, S.Tsunawaki, K.Abe, K.Shirasuna and K.Nomoto: "An accumulation of human Tlymphotrophic virus type I(HTLV-I)-infected T cells in the salivary glands of patients with HTLV-I-assoc

Y.Ohyama、S.Nakamura、H.Hara、M.Shinohara、M.Sasaki、A.Ikebe-Hiroki、T.Mouri、S.Tsunawaki、K.Abe、K.Shirasuna 和 K.Nomoto：“积累

Y., Ohyama, S., Nakamura, H., Hara, M., Sinohara M., Sasaki, A., Ikebe-Hiroki, T., Mouri, S., Tsunawaki K., Abe, K,Shirasuna and K., Nomoto: "An accumulation of human Tlymphotrophic virus type I (HTLV-I) -infected T cells in the salivary glands of patient

Y.、Ohyama, S.、Nakamura, H.、Hara, M.、Sinohara M.、Sasaki, A.、Ikebe-Hiroki, T.、Mouri, S.、Tsunawaki K.、Abe, K、Shirasuna 和 K