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Immunohistochemical observation for oncogene and tumor suppressor gene products and extracellural materials in tumors in oral cavity.

口腔肿瘤癌基因、抑癌基因产物及细胞外物质的免疫组织化学观察。

基本信息

批准号：
07457499
负责人：
NAITOH Ryouji
金额：
$ 3.78万
依托单位：
Asahi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Immunohistochemical studies for oncoprotein, adhesion molecules and extracellular matrix (ECM) were carried out in oral carcinomas and salivary gland tumors.Mutant p53 tumor suppressor gene product was found in 2 of 9 cases in epithelial displasia and 30 of 38 cases in squamous cell carcinoma (SCC) of oral mucosa. Immunohistochemical localization was confined to cell nuclei, and limited in epithelial dysplasia, and basal and parabasal area in SCC.Ratio of mutant p53 positive cells in SCC cells was correlated with malignancy of SCCs.E-cadherin (E-cad) staining in oral SCC showed different manner with their cell differntiation. E-cad was found all cell membrane in highly differentiated SCC and negative in poorly differentiated tumor. In moderately differentiated SCC,tumor cells in central foci have positive staining and peripheral area negative. The tumor cell differntiation and the degree of E-cad expression were significantly corresponded.During prenatal development of human salivary g … More lands, laminin (LN) and type IV collagen (CL IV) were observed in the basement membrane of salivary gland duct and acini from 16-18 weeks and ataining intensity was more prominent in intermediate developing stage (19-32 weeks). The reaction products were less prominent in late development stage (33-34 weeks). Tenascin (TN) was present in epithelial-mesenchymal interface of developing salivary gland ducts from 10 weeks. In the late stage, TN was reduced around the striated ducts and negative around the intercalated ducts and acinar cells. In adult salivary gland, reaction for LN and CL IV were confined to basement membrane of ducts and acini, and TN was existed in peripheral connective tissue of striated excretory ducts. Fibronectin (FN) stained in salivary duct cells and stromal connective tissue. In pleomorphic adenoma (PA) and adenoid cystic carcinoma (ACC), modified myoepithelial cells (MMC), stained positive reaction for LN,FN,and TN.MMC may produce and secreat ECMs in myxomatou, hyalinous, and chondroidal structure of PA and those in pseudecysts of ACC.In vitro study, human tongue carcinoma cells line (SCCKN), human salivary adenocarcinoma cell (SGT-1) and NIH3T3-3 had enhanced secretion of TN in the presence of transforming growth factor beta in a dose dependent manner and TN itself was found to possess a growth-enhancing activity. In cellular adhesion for 3 cells test suggested that all cells adhere and spread well on FN and LN,however, cells attach poorly on TN alone. When SCCKN and SGT-1 grown on FN/TN substrate induced collagenase. Enhanced TN in neoplastic lesions may have potential implications for tumor growth, differentiation, cellular adhesion, invasion and metastasis. Less

应用免疫组织化学方法对口腔癌和涎腺肿瘤的癌蛋白、粘附分子和细胞外基质（ECM）进行了研究，发现9例上皮性涎腺炎中有2例p53抑癌基因产物突变，38例口腔鳞癌中有30例p53抑癌基因产物突变。免疫组化定位于细胞核，局限于上皮异型增生区、基底区和副基底区，突变型p53阳性细胞比例与SCC恶性程度相关，E-cadherin（E-cad）染色随SCC细胞分化程度不同而呈不同的表达方式。E-cad在高分化鳞癌中呈全膜表达，在低分化鳞癌中呈阴性表达。在中等分化的SCC中，中央病灶的肿瘤细胞呈阳性染色，而周边区域呈阴性染色。肿瘤细胞分化程度与E-cad表达程度呈显著正相关。 ...更多信息 16-18周时，涎腺导管和腺泡基底膜可见层粘连蛋白（LN）和Ⅳ型胶原（CL Ⅳ），且在中期（19-32周），LN和CL Ⅳ的表达增强。发育后期（33-34周）反应产物不明显。生腱蛋白（TN）从10周开始出现在发育中的涎腺导管的上皮-间充质界面。晚期纹管周围TN减少，闰管和腺泡细胞周围TN呈阴性。LN和CL IV在成人涎腺中的反应局限于导管和腺泡的基底膜，TN存在于纹状排泄管周围的结缔组织中。纤维连接蛋白（FN）在涎腺导管细胞和间质结缔组织中染色。在多形性腺瘤（PA）和腺样囊性癌（ACC）中，变性肌上皮细胞（MMC）对LN、FN和TN呈阳性反应，MMC可在PA的粘液瘤、透明质、软骨样结构和ACC的假囊肿中产生和分泌ECM。人唾液腺腺癌细胞（SGT-1）和NIH 3 T3 -3在转化生长因子β存在下以剂量依赖的方式增强TN的分泌，并且发现TN本身具有生长促进活性。3细胞粘附试验表明，所有细胞在FN和LN上粘附和铺展良好，而在TN上粘附较差。SCCKN和SGT-1在FN/TN底物上生长时，诱导胶原酶。肿瘤病变中TN的增强可能对肿瘤的生长、分化、细胞粘附、侵袭和转移具有潜在的意义。少