ODC gene overexpression and carcinogenesis of intestinal epithelial cells

ODC基因过表达与肠上皮细胞癌变

• 批准号: 07457594
• 负责人: BAMBA Tadao
• 金额: $ 0.19万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457594/
• 关键词: ODC; colon cancer; mutation; antizyme

Most of colon cancer tissue poses high ODC activity and putrescine content. We chose some of cancer tissues with extensively high ODC activity, extracted RNA and mede ODC cDNA by RT-PCR.Unfortunately, all of ODC cDNA which were sequenced did not has mutation. We speculate that much higher ODC activity of colon cancer was associated with the stability of ODC protein. We will investigate the relation antizyme mutation and ODC activity.

大多数结肠癌组织中ODC活性和腐胺含量较高。我们选择了ODC活性高的癌组织，提取RNA，用RT-PCR方法扩增ODC cDNA，测序结果表明，所有ODC cDNA均未发生突变。我们推测结肠癌组织中ODC活性的升高与ODC蛋白的稳定性有关。我们将研究抗酶突变与ODC活性的关系。