Clinical application of evaluation of complement protein levels in the exocrine pancreas to determine the pancreatic disease activity
评估胰腺外分泌补体蛋白水平以确定胰腺疾病活动度的临床应用
基本信息
- 批准号:09470135
- 负责人:
- 金额:$ 4.61万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The complement system is a potent effector of the normal immune and inflammatory responses. Invasion by microorganisms, combined with a reflux of duodenal contents, may be important factors in the pathogenesis of acute and chronic pancreatitis. However, little is known about the mechanism underlying the immunologic protection of the exocrine pancreas.The secretory IgA system has been shown to be present in pancreatic fluids. In addition, we found that several complement proteins are secreted into pancreatic juices. From our results, complement C3, C4 and factor B are present in pancreatic juice at high concentration. Furthermore, several pancreatic cancer cell lines are secreting these complement proteins in responses to IL-1β, TNF-α and IFN-γ suggesting that ductal epithelial cells are possible biosynthetic site of complement proteins. We also tried to detect complement mRNA expression in situ. Although in situ hybridization in the pancreatic tissue is difficult, we detected complement mRNA expression in some samples. In addition, we observed high mRNA expression in some samples of chronic pancreatitis. When comparing the complement levels in pancreatic juices between normal and chronic pancreatitis, we detect high amounts of C3 in samples of chronic pancreatitis. Thus, our investigation presented a possibility that determination of complement proteins in pancreatic juices is useful to evaluate disease activity, such as chronic pancreatitis.
补体系统是正常免疫和炎症反应的有效效应子。微生物的侵入,结合十二指肠内容物的反流,可能是急性和慢性胰腺炎发病机制的重要因素。然而,胰腺外分泌系统的免疫保护机制尚不清楚,分泌型伊加系统已被证实存在于胰液中。此外,我们发现几种补体蛋白被分泌到胰液中。从我们的结果来看,补体C3、C4和B因子以高浓度存在于胰液中。此外,几种胰腺癌细胞系响应于IL-1β、TNF-α和IFN-γ而分泌这些补体蛋白,这表明导管上皮细胞是补体蛋白的可能生物合成位点。我们还试图检测补体mRNA的表达原位。虽然在胰腺组织中的原位杂交是困难的,我们检测到一些样品中的补体mRNA的表达。此外,我们在一些慢性胰腺炎样本中观察到高mRNA表达。当比较正常和慢性胰腺炎之间的胰液中的补体水平时,我们在慢性胰腺炎样品中检测到大量的C3。因此,我们的研究提出了一种可能性,即测定胰液中的补体蛋白有助于评估疾病的活动性,如慢性胰腺炎。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Andoh A.,Bamba T. et al.: "Counter-regulatory effect of sodium butyrate on tumor necrosis factor-alpha(TNF-α)-induced complement C_3 and factor B"Clin Exp Immunol. 118. 23-29 (1999)
Andoh A.,Bamba T.等人:“丁酸钠对肿瘤坏死因子-α(TNF-α)诱导的补体 C_3 和因子 B 的反调节作用”Clin Exp Nutritionl. 118. 23-29 (1999)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Andoh A, Bamba T, Sasaki M.: "Physiological and anti-inflammatory roles and dietary fiber and butyrate in intestinal functions"JPEN J. Parenter. Enteral Nutr.. 23. 70-73 (1999)
Andoh A、Bamba T、Sasaki M.:“肠道功能中的生理和抗炎作用以及膳食纤维和丁酸盐”JPEN J. Parenter。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kitamura K, Andoh A, Inoue T, Amakata Y, Hodohara K, Fujiyama Y, Bamba T.: "Sodium Butyrate blocks interferon-gamma (IFN-γ)-induced biosynthesis of MHC class III gene products (complement C4 and factor B) in human fetal intestinal epithelial cells"Clin Ex
Kitamura K、Andoh A、Inoue T、Amakata Y、Hodohara K、Fujiyama Y、Bamba T.:“丁酸钠可阻断干扰素 γ (IFN-γ) 诱导的 MHC III 类基因产物(补体 C4 和因子 B)的生物合成在人胎儿肠上皮细胞中“Clin Ex
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Araki Y, Andoh A, Fujiyama Y, Bamba T.: "Development of dextran sulphate sodium-induced experimental colitis is suppressed in genetically mast cell-deficient Ws/Ws rats"Clin Exp Immunol.. 119(2). 264-269 (1999)
Araki Y、Andoh A、Fujiyama Y、Bamba T.:“在遗传肥大细胞缺陷的 Ws/Ws 大鼠中,右旋糖酐硫酸钠诱导的实验性结肠炎的发展受到抑制”Clin Exp Immunol.. 119(2)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Bamba T.,Andoh A. et al.: "Regulation of mucosal inflammation and Clinical outcome in inflammatory bowel disease"Bioregulation and Its Disorders in the Gastrointestinal tract. 1. 243-252 (1998)
Bamba T.、Andoh A. 等人:“炎症性肠病的粘膜炎症调节和临床结果”胃肠道的生物调节及其紊乱。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
BAMBA Tadao其他文献
BAMBA Tadao的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('BAMBA Tadao', 18)}}的其他基金
the role of short-chain Fatty acids in the intestine
短链脂肪酸在肠道中的作用
- 批准号:
12470121 - 财政年份:2000
- 资助金额:
$ 4.61万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
ODC gene overexpression and carcinogenesis of intestinal epithelial cells
ODC基因过表达与肠上皮细胞癌变
- 批准号:
07457594 - 财政年份:1995
- 资助金额:
$ 4.61万 - 项目类别:
Grant-in-Aid for Scientific Research (B)